Application | Comment | Organism |
---|---|---|
medicine | the enzyme catalyses the hydrolysis of leukotriene A4 into the proinflammatory substance leukotriene B4 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant enzymes expressed in Escherichia coli | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
wild-type enzyme and mutant D375N | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D368N | epoxide hydrolase activity: 72% of Km of wild type enzyme, aminopeptidase activity: 107% of Km of wild type enzyme | Homo sapiens |
D371N | epoxide hydrolase activity: 51% of Km of wild type enzyme, aminopeptidase activity: 77% of Km of wild type enzyme | Homo sapiens |
D373N | epoxide hydrolase activity: 43% of Km of wild type enzyme, aminopeptidase activity: 101% of Km of wild type enzyme | Homo sapiens |
D375A | near-complete loss of the epoxide hydrolase activity, aminopeptidase activity exhibits unsaturable enzyme kinetic | Homo sapiens |
D375E | complete loss of the epoxide hydrolase activity, aminopeptidase activity: 152% of Km of wild type enzyme | Homo sapiens |
D375N | complete loss of the epoxide hydrolase activity, aminopeptidase activity exhibits unsaturable enzyme kinetic | Homo sapiens |
H139Q | epoxide hydrolase activity: 167% of Km of wild type enzyme, aminopeptidase activity: 359% of Km of wild type enzyme | Homo sapiens |
Q134A | epoxide hydrolase activity exhibits unsaturable enzyme kinetics within the range observed, aminopeptidase activity: 157% of Km of wild type enzyme | Homo sapiens |
Q134L | significantly increased epoxide hydrolase activity, aminopeptidase activity: 87% of Km of wild type enzyme | Homo sapiens |
Q134N | epoxide hydrolase activity: 148% of Km of wild type enzyme, aminopeptidase activity: 108% of Km of wild type enzyme | Homo sapiens |
Y267F | epoxide hydrolase activity: 58% of Km of wild type enzyme, aminopeptidase activity: 64% of Km of wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.006 | 0.028 | leukotriene A4 | pH 8.0, wild-type enzyme in comparison to mutant enzymes | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | Homo sapiens | biosynthesis of leukotriene B4 | leukotriene B4 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Drosophila melanogaster | - |
- |
- |
Homo sapiens | P09960 | - |
- |
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant enzymes | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
leukotriene A4 + H2O = leukotriene B4 | Asp375 acts as a critical determinant for the stereoselective introduction of the 12R-hydroxyl group, possibly assisted by Gln134 and thus the biological activity of leukotriene B4 | Homo sapiens | |
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Saccharomyces cerevisiae | |
leukotriene A4 + H2O = leukotriene B4 | bifunctional enzyme acting as an epoxide hydrolase and also as an aminopeptidase | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? | |
leukotriene A4 + H2O | biosynthesis of leukotriene B4 | Homo sapiens | leukotriene B4 | - |
? | |
additional information | - |
Saccharomyces cerevisiae | ? | - |
? | |
additional information | - |
Caenorhabditis elegans | ? | - |
? | |
additional information | - |
Homo sapiens | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | 0.47 | leukotriene A4 | pH 8.0, wild-type enzyme in comparison to mutant enzymes | Homo sapiens |