EC Number |
---|
3.3.2.6 | - |
3.3.2.6 | enzyme monomer from leukocytes |
3.3.2.6 | Ni-Sepharose column chromatography, and MonoQ column chromatography |
3.3.2.6 | recombinant enzyme |
3.3.2.6 | recombinant enzyme, expression in Escherichia coli as a fusion protein with ten additional amino acids at the amino terminus |
3.3.2.6 | recombinant His6-tagged enzyme by nickel affinity chromatography and gel filtration |
3.3.2.6 | using metal affinity chromatography, the His tag is removed by digestion with His6-TEV protease |
3.3.2.6 | wild-type and mutant enzymes |