Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM101 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
mutant enzyme E296Q in complex with inhibitors RB3040 and RB3041, and substrate, liquid-liquid diffusion method, using 28% (w/v) polyethylene glycol, 50 mM Na acetate, 100 mM imidazole (pH 6.8), and 5 mM YbCl3 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D375A | mutant with reduced activity | Homo sapiens |
E296Q | mutant with severely reduced activity | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(S)-2-((S)-2-(2-(((R)-1-amino-2-phenylethyl)(hydroxy)phosphoryl)acetamido)-3-phenylpropanamido)-3-phenylpropanoic acid | RB3041, tight slow-binding LTA4H inhibitor | Homo sapiens | |
(S)-2-((S)-2-(2-(((R)-1-aminoethyl)(hydroxy)phosphoryl)acetamido)-3-(biphenyl-4-yl)propanamido)propanoic acid | RB3040, tight slow-binding LTA4H inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.3 | - |
Arg-Ser-Arg | wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
1.5 | - |
L-Arg-p-nitroanilide | wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
1.9 | - |
L-Ala-p-nitroanilide | mutant enzyme E296Q, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
1.9 | - |
L-Ala-p-nitroanilide | wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
2.2 | - |
L-Arg-p-nitroanilide | mutant enzyme E296Q, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
3 | - |
L-Ala-p-nitroanilide | mutant enzyme D375A, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | the enzyme contains zinc | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P09960 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Arg-Ala-Arg + H2O | - |
Homo sapiens | ? | - |
? | |
Arg-Ser-Arg + H2O | - |
Homo sapiens | ? | - |
? | |
L-Ala-p-nitroanilide + H2O | - |
Homo sapiens | L-Ala + p-nitroaniline | - |
? | |
L-Arg-p-nitroanilide + H2O | - |
Homo sapiens | L-Arg + p-nitroaniline | - |
? | |
leukotriene A4 + H2O | - |
Homo sapiens | leukotriene B4 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
leukotriene A4 hydrolase | - |
Homo sapiens |
LTA4H | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
L-Ala-p-nitroanilide | mutant enzyme E296Q, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
0.002 | - |
L-Arg-p-nitroanilide | mutant enzyme E296Q, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
0.5 | - |
L-Ala-p-nitroanilide | mutant enzyme D375A, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
0.56 | - |
Arg-Ser-Arg | wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
1.7 | - |
L-Ala-p-nitroanilide | wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens | |
1.9 | - |
L-Arg-p-nitroanilide | wild type enzyme, 500 mM KCl in 250 mM Tris buffer (pH 7.5) | Homo sapiens |