84% inhibition of tobacco mosaic virus on Nicotiana glutinosa leaves, determined by comparing lesions on BBAP1 treated with untreated leaves as control
84% inhibition of tobacco mosaic virus on Nicotiana glutinosa leaves, determined by comparing lesions on BBAP1 treated with untreated leaves as control
specific activity 257.8 units for cinnamomin, 213.2 units for camphorin, 1 unit is the amount of protein necessary for inhibiting protein synthesis by 50% in 0.1 ml rabbit-reticulocyte lysate
about 90% retained protein synthesis activity in assay cells and about 70% fluorescence by 0.001 ng/ml Stx2, about 50% activity and fluorescence by 0.1 ng/ml Stx2, about 30% activity and fluorescence by 100 ng/ml Stx2, Shiga toxin type 1 and 2 producing cell culture of strain RM1697 has similar dose-response curve as Stx2
marmorin inhibits proliferation of hepatoma Hep G2 cells (IC50 = 0.00015 mM), breast cancer MCF-7 cells (IC50 = 0.005 mM), HIV-1 reverse transcriptase activity (IC50 = 0.03 mM), and translation in the rabbit reticulocyte lysate system (IC50 = 0.0000007 mM), no inhibitory action on embryonic liver WRL-68 cells, no antifungal, mitogenic/antimitogenic, protease, protease inhibitory, RNase, and nitric oxide-inducing activities
momordin: increase in cells that show DNA damage in both species, reduced weight in Anticarsia gemmatalis at day 10 with 40 microg, in Spodoptera frugiperda superoxide dismutase inhibition to 20% with 40 microg
PAP-S: increase in cells that show DNA damage in both species, reduced weight gain of Spodoptera frugiperda (not Anticarsia gemmatalis) on day 4 with 40 microg, increased weight gain with 20 microg in Spodoptera frugiperda, not significant increase in weight gain in Anticarsia gemmatalis fed with 40 microg, reduced weight in Anticarsia gemmatalis and Spodoptera frugiperda at day 10 with 40 microg, 57.1% mortality in Anticarsia gemmatalis, in Anticarsia gemmatalis 30-40% reduced level of lipid peroxidation with 40 microg, in Spodoptera frugiperda superoxide dismutase inhibition to 20% and catalase activity increase with 40 microg
specific activity 5000 units/mg of protein in old leaves, 6920 in young leaves, 6900 in stems, 37000 in roots, 640 in flower petals, 5400 in flower sepals, 2700 in flower ovaries, 1100 in flower pistils, 7000 in fruits skins, 365 in fruits cores, 17 in seeds at stage I, 135000 in seeds at stage II and III, and 120000 at stage IV, one unit is defined as the amount of protein necessary to inhibit protein synthesis by 50% in 1 ml of rabbit-reticulocyte lysate, specific activity of purified protein from seeds 633000, from roots 69200
no effect on protein synthesis below 4 microM saporin-6, 20% inhibition with 4 microM after 24 h, at 10 and 18 h ID50 greater than 8 microM, at 24 h 7 microM
saporin S-6: increase in cells that show DNA damage in both species, reduced weight gain of Anticarsia gemmatalis and Spodoptera frugiperda on day 4 with 40 microg, increased weight gain with 20 microg in Spodoptera frugiperda, reduced weight in Spodoptera frugiperda at day 10 fed with 20 microg, in Spodoptera frugiperda superoxide dismutase inhibition to 20% with 40 microg
lychnin: increase in cells that show DNA damage in both species, reduced weight gain of Spodoptera frugiperda (not Anticarsia gemmatalis), increased weight gain with 20 microg in Spodoptera frugiperda, reduced weight in Anticarsia gemmatalis at day 10 with 40 microg and in Spodoptera frugiperda fed with 20 microg, 42.8% mortality in Anticarsia gemmatalis, in Anticarsia gemmatalis 30-40% reduced level of lipid peroxidation with 40 microg, in Spodoptera frugiperda superoxide dismutase inhibition to 20% with 40 microg
gelonin: increase in cells that show DNA damage in both species, reduced weight gain of Spodoptera frugiperda (not Anticarsia gemmatalis) on day 4 with 40 microg, no significant increase in weight gain in Anticarsia gemmatalis fed with 40 microg, reduced weight in Anticarsia gemmatalis and Spodoptera frugiperda at day 10 with 40 microg, in Anticarsia gemmatalis 30-40% reduced level of lipid peroxidation with 40 microg, in Spodoptera frugiperda superoxide dismutase inhibition to 20% and reduced catalase activity with 40 microg