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3.2.2.22: rRNA N-glycosylase

This is an abbreviated version!
For detailed information about rRNA N-glycosylase, go to the full flat file.

Word Map on EC 3.2.2.22

Reaction

A4324 in 28S rRNA
+
H2O
=
deadenylated 28S rRNA
 +
adenine

Synonyms

(ribosome-inactivating protein)-like protein, abelesculin, ABRaA, abrin, abrin-a A chain, Abrus precatorius agglutinin, alpha-MC, alpha-momorcharin, alpha-pisavin, antiviral protein DAP-30, aralin, B-32, balsamin, BBAP1, BE27, beta-momorcharin, beta-pisavin, Bougainvillea xbuttiana antiviral protein 1, bouganin, camphorin, CAP30, cinnamomin, Cochinin B, cytotoxic ribosome-inactivating lectin, D6D54_07625, D6D54_08385, D6D54_08880, depurinating rRNA N-glycosidase, dianthin 30, dianthin 32, dianthin-30, diphtheria toxin, ebulin b, ebulitin alpha, ebulitin beta, ebulitin gamma, gelonin, gysophilin, hispin, IRAb, IRIP, Iris agglutinin b, Iris ribosome-inactivating protein, JIP60, karasurin-A, KML, lamjapin, luffaculin I, luffin P1, lychnin, MAP, marmorin, ME1, Mirabilis antiviral protein, mistletoe lectin I, mistletoe lectin II, mistletoe lectin III, ML-I, MLI, MLII, MLIII, MOD, modeccin, momorcochin, momorcochin-S, momordin, momordin I, momordin II, momorgrosvin, More, N-glycosidase, n-TCS, neo-trichosanthin, nigrin b, nigritin f1, nigritin f2, PAG, PAGase, PAP, PAP-I, PAP-II, PAP-III, PAP-R, PAP-S, PD-L, PD-L1, PD-L4, pepocin, PGase, pokeweed anti-viral protein, pokeweed antiviral protein, polynucleotide:adenosine glycosidase, polynucleotide:adenosine glycosylase, porrectin, RA, RCA-II, ribosomal inactivating protein, ribosomal ribonucleate N-glycosidase, ribosome inactivating protein, ribosome-inactivating protein, ribosome-inactivating protein 3, ribosome-inactivating protein type II, ribosome-inactivating proteins, ribosome-inactivating type II protein, ribosome-specific N-glycosidase, ricin, ricin A, ricin A chain, ricin A-chain, ricin toxin A-chain, ricin-A-chain, ricin-like protein, Ricinus communis agglutinin, Ricinus communis agglutinin II, RIP, RIP-1, RIP1, RIP2, RIPII, riproximin, RLP1, RLP2, RLP3, RLP4, RLP5, RLP6, RNA N-glycosidase, RNA-NGA, RNA-specific N-glycosidase, RNase Bo, Rpx, rRNA N-glycosidase, RTA, Sambucus nigra agglutinin, Sambucus nigra agglutinin I, SAP, saporin, saporin 6, saporin S-6, saporin S6, saporin-6, saporin-L1, saporin-S6, saporins, SBA, SCO7092, SEPIN, Shiga toxin, Shiga toxin 1, Shiga toxin 1 subunit A, Shiga toxin 2, Shiga toxin 2 subunit A, Shiga toxin type 1, Shiga toxin type 2, Shiga toxin-1, shiga-like toxin, shiga-like toxin I, sieboldin-b, single-chain ribosome-inactivating protein, SLT-I, SLT-II, SLT-IIv, SNA, SNA-I, SNA-V, SnaI, SNAIf, SNAV, SNLRP, SoRIP1, SoRIP2, stenodactylin, Stx, Stx type 1, Stx-1, Stx1, Stx1A, Stx2, Stx2A, Stx2dact, StxB, TCS, trichoanguin, trichomaglin, trichosanthin, Trichosanthrip, trichosnathin, TRIP, tritin-L, tritin-S, TYchi, type 1 ribosome inactivating protein, type 1 ribosome-inactivating protein, type 1 RIP, type 2 ribosome inactivating protein, type 2 ribosome-inactivating protein, type 2 RIP, type 2RIP, type 3 RIP, type I ribosome inactivating protein, type I ribosome-inactivating protein, type I RIP, type II ribosome inactivating protein, type II ribosome-inactivating protein, type II RIP, type III ribosome-inactivating protein, type III RIP, type-1 ribosome-inactivating protein, type-1 RIP, type-2 ribosome-inactivating protein, type-2 RIP, VAA-I, viscinum, Viscum album agglutinin I, viscumin, volkensin

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.22 rRNA N-glycosylase

Molecular Weight

Molecular Weight on EC 3.2.2.22 - rRNA N-glycosylase

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10000
-
1 * 10000, SDS-PAGE
13000
-
x * 13000, SDS-PAGE, x * 10964.6, mass spectrometry
18700
-
beta-pisavin, gel filtration
20500
-
alpha-pisavin, gel filtration
21000
22000
-
1 * 40000 + 1 * 22000, chain B and chain A, SDS-PAGE
22500
-
1 * 22500, camphorin, SDS-PAGE
23000
-
type I RIP, camphorin, gel filtration
23570
-
nigritin f2, mass spectrometry
24100
-
nigritin f1, mass spectrometry
24670
Trichosanthes lepiniate
-
electrospray ionization mass spectroscopy
25000
-
gel filtration
26000
27000
27500 - 30100
-
7 major saporins, gel filtration
27700
28000
28478
-
1 * 28478, ML-I subunit A + 1 * 28981, ML-I subunits B, sequence calculation
28981
-
1 * 28478, ML-I subunit A + 1 * 28981, ML-I subunits B, sequence calculation
29000
29094
x * 29094, calculated from amino acid sequence
30000
30200
30360
PD-L 3 measured with ESI-Q-TOF MS, including two disulfide bridges (-4) and two glycosidic chains (+2342), corresponding to calculated value of amino acids 29190 Da
30500
30700
-
1 * 30700, momorchochin-S, SDS-PAGE
30900
-
1 * 30900, SO-R2, SO-R3, and SO-S%, SDS-PAGE
31000
31200
C-terminal His-tag version
31560
PD-L 2 measured with ESI-Q-TOF MS, including two disulfide bridges (-4) and two glycosidic chains (+2342), corresponding to calculated value of amino acids 29222 Da
31600
32000
32500
-
x * 32500, mature enzyme, SDS-PAGE
33000
33500
-
1 * 30500 + 1 * 33500, A-chain and B-chain, SDS-PAGE
34500
-
1 * 30500 + 1 * 34500, cinnamomin, A- and B-chain, SDS-PAGE
34600
-
1 * 35000, SDS-PAGE, 1 * 34600, calculated for mature protein, 1 * 62000, calculated for full-length protein including N-terminal leader sequence, pectin methylesterase inhibitor domain, pectin esterase domain and pectin esterase signature sequences 1 and 2
35000
35490
calculated value of 319 amino acids of BBAP1, signal peptide sequence of 36 amino acids of 3.89 kDa at N-terminus
36000
40000
-
1 * 40000 + 1 * 22000, chain B and chain A, SDS-PAGE
42500
SDS-PAGE, maltose-binding protein as partial degradation product of fusion protein
5226
-
MALDI-TOF MS analysis
58000
-
gel filtration
59400
-
time-of-flight mass spectrometry
6000
-
SDS-PAGE
60000
61000
62000
-
1 * 35000, SDS-PAGE, 1 * 34600, calculated for mature protein, 1 * 62000, calculated for full-length protein including N-terminal leader sequence, pectin methylesterase inhibitor domain, pectin esterase domain and pectin esterase signature sequences 1 and 2
62912
-
x * 62912, MALDI-TOF mass spectrometry
62925
x * 62925, MALDI-TOF
66000
-
holoenzyme, native PAGE
78000
SDS-PAGE, recombinant fusion protein of BBAP1 and maltose-binding protein
9567
-
MALDI-TOF MS, SDS-PAGE