3.2.2.22: rRNA N-glycosylase

This is an abbreviated version, for detailed information about rRNA N-glycosylase, go to the full flat file.

Reaction

hydrolysis of the N-glycosylic bond at A-4324 in 28S rRNA from rat ribosomes =

Synonyms

(ribosome-inactivating protein)-like protein, abelesculin, ABRaA, abrin, abrin-a A chain, Abrus precatorius agglutinin, Agglutinin, alpha-MC, alpha-momorcharin, alpha-pisavin, aralin, B-32, balsamin, BBAP1, beta-momorcharin, beta-pisavin, Bougainvillea xbuttiana antiviral protein 1, bouganin, camphorin, CAP30, cinnamomin, Cochinin B, cytotoxic ribosome-inactivating lectin, depurinating rRNA N-glycosidase, dianthin 30, dianthin 32, diphtheria toxin, ebulin b, ebulitin alpha, ebulitin beta, ebulitin gamma, gelonin, gysophilin, hispin, IRAb, IRIP, Iris agglutinin b, Iris ribosome-inactivating protein, JIP60, karasurin-A, KML, lamjapin, luffaculin I, luffin P1, lychnin, MAP, marmorin, ME1, Mirabilis antiviral protein, mistletoe lectin I, mistletoe lectin II, mistletoe lectin III, ML-I, MLI, MLII, MLIII, MOD, modeccin, momorcochin, momorcochin-S, momordin, momordin I, momordin II, momorgrosvin, More, N-glycosidase, n-TCS, neo-trichosanthin, nigrin b, nigritin f1, nigritin f2, PAG, PAGase, PAP, PAP-I, PAP-II, PAP-III, PAP-R, PAP-S, PD-L, PD-L1, PD-L4, pepocin, PGase, pokeweed anti-viral protein, pokeweed antiviral protein, polynucleotide:adenosine glycosidase, polynucleotide:adenosine glycosidase activity, polynucleotide:adenosine glycosylase, porrectin, RA, RCA-II, ribosomal inactivating protein, ribosomal ribonucleate N-glycosidase, ribosome inactivating protein, ribosome-inactivating protein, ribosome-inactivating protein 3, ribosome-inactivating protein type II, ribosome-inactivating proteins, ribosome-inactivating type II protein, ribosome-specific N-glycosidase, ricin, ricin A, ricin A chain, ricin A-chain, ricin toxin A-chain, ricin-A-chain, ricin-like protein, Ricinus communis agglutinin, Ricinus communis agglutinin II, RIP, RIP-1, RIP1, RIP2, RIPII, riproximin, RLP1, RLP2, RLP3, RLP4, RLP5, RLP6, RNA N-glycosidase, RNA-NGA, RNA-specific N-glycosidase, Rpx, rRNA N-glycosidase, RTA, Sambucus nigra agglutinin, Sambucus nigra agglutinin I, SAP, saporin, saporin 6, saporin S-6, saporin S6, saporin-6, saporin-L1, saporin-S6, saporins, SBA, SCO7092, Shiga toxin, Shiga toxin 1, Shiga toxin 1 subunit A, Shiga toxin 2, Shiga toxin 2 subunit A, Shiga toxin type 1, Shiga toxin type 2, Shiga toxin-1, shiga-like toxin, shiga-like toxin I, sieboldin-b, single-chain ribosome-inactivating protein, SLT-I, SLT-II, SLT-IIv, SNA, SNA-I, SNA-V, SnaI, SNAIf, SNAV, SNLRP, SoRIP1, SoRIP2, stenodactylin, Stx, Stx type 1, Stx-1, Stx1, Stx1A, Stx2, Stx2A, Stx2dact, StxB, TCS, trichoanguin, trichomaglin, trichosanthin, Trichosanthrip, trichosnathin, TRIP, tritin-L, tritin-S, TYchi, type 1 ribosome inactivating protein, type 1 ribosome-inactivating protein, type 1 RIP, type 2 ribosome inactivating protein, type 2 ribosome-inactivating protein, type 2 RIP, type 2RIP, type 3 RIP, type I ribosome inactivating protein, type I ribosome-inactivating protein, type I RIP, type II ribosome inactivating protein, type II ribosome-inactivating protein, type II RIP, type III ribosome-inactivating protein, type III RIP, type-1 ribosome-inactivating protein, type-1 RIP, type-2 ribosome-inactivating protein, type-2 RIP, VAA-I, viscinum, Viscum album agglutinin I, viscumin, volkensin

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.22 rRNA N-glycosylase

KM Value

KM Value on EC 3.2.2.22 - rRNA N-glycosylase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
28S rRNA within the native ribosome
-
-
-
0.01
5'-dG1dC2dG3dC4dG5A6dG7dA8dG9dC10dG11dC12-3'
-
37C
0.005
5'-dG1dC2dG3dC4dG5dA6dG7A8dG9dC10dG11dC12-3'
-
37C
0.0152
5'-dG1dC2dG3dC4dG5dA6G7dA8dG9dC10dG11dC12-3'
-
37C
0.0094
5'-dG1dC2dG3dC4G5dA6dG7dA8dG9dC10dG11dC12-3'
-
37C
0.1
5'-G1C2G3C4dG5A6dG7A8G9C10G11C12-3'
-
37C
0.0007
5'-G1C2G3C4dG5A6G7A8G9C10G11C12-3'
-
37C
0.0018
5'-G1C2G3C4dG5A6G7dA8G9C10G11C12-3'
-
37C
0.0009
5'-G1C2G3C4G5A6dG7A8G9C10G11C12-3'
-
37C
0.0027
5'-G1C2G3C4G5A6G7A8G9C10G11C12-3
-
37C
0.0057
5'-G1C2G3C4G5A6G7dA8G9C10G11C12-3'
-
37C
0.0097
5'-G1C2G3C4G5A6G7mA8G9C10G11C12-3'
-
37C
0.0063
5'-G1C2G3C4G5A6mG7A8G9C10G11C12-3'
-
37C
0.032
5'-G1C2G3C4G5dA6G7A8G9C10G11C12-3'
-
37C
0.3
5'-G1C2G3C4G5mA6G7A8G9C10G11C12-3'
-
37C
0.0029
5'-G1C2G3C4mG5A6G7A8G9C10G11C12-3'
-
37C
0.095 - 0.36
A-10 RNA
-
0.0103
A-14 2-dA stem-loop RNA/DNA hybrid
-
pH 4.0
-
0.039
cyclic dG-(N-benzyl-aza-deoxyribosyl)-dGdA
-
pH 4.0, 37C
0.097
cyclic dGdAdGdA
-
pH 4.0, 37C
0.038
cyclic GdAGA
-
pH 4.0, 37C
0.082
cyclic oxime GAGA
-
pH 7.7, 20C
0.046
cyclic phosphorothioyl dGdAdGdA
-
pH 4.0, 37C
0.112
dGdAdGdA
-
pH 4.0, 37C
0.0084
dGdCdGdCdGdAdGdAdGdCdGdC
-
pH 4.0, 37C
0.065
GdAGA
-
pH 4.0, 37C
0.266
linear GAGA
-
pH 7.7, 20C
0.0058
naked 28S rRNA
-
-
-
0.065 - 0.639
Poly(A)
0.0001
rabbit reticulocyte 80S rRNA
-
pH 4.0
-
0.000055 - 0.0058
rRNA
0.00464
sarcin/ricin domains of Escherichia coli 23S rRNA
-
pH 5.0
-
0.00457
sarcin/ricin domains of Rattus norvegicus 28S rRNA
-
pH 5.0
-
0.0022
stem-loop substrate A-10
-
pH 4.0
-
0.00006
yeast 60S rRNA
-
pH 4.0
-
additional information
additional information
-