Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant ricin A chains in Escherichia coli strain JM101 | Ricinus communis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of mutant ricin A chains by insertional mutagenesis, the mutants possessing the specific 25-residue inserted internal peptide, derived from maize proRIP, show about 300fold reduced catalytic activity | Ricinus communis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Ricinus communis | the wild-type holotoxin ricin is lethal for the target cell, e.g. Verocells, due to inactivation of ribosomes and inhibition of protein synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant ricin A chains from Escherichia coli strain JM101 by anion exchange chromatography and cobalt affinity chromatography to homogeneity | Ricinus communis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
protein synthesis inhibition and cytotoxicity of wild-type and mutant ricin A chains, overview | Ricinus communis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the wild-type holotoxin ricin is lethal for the target cell, e.g. Verocells, due to inactivation of ribosomes and inhibition of protein synthesis | Ricinus communis | ? | - |
? | |
ribosomes + H2O | substrate from yeast | Ricinus communis | ? | - |
? | |
rRNA + H2O | product determination using aniline coupling | Ricinus communis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the heterodimeric enzyme consists of the catalytic ricin A chain, RTA, and the cabohydrate-binding B chain, RTB | Ricinus communis |
Synonyms | Comment | Organism |
---|---|---|
ricin | - |
Ricinus communis |
RIPII | - |
Ricinus communis |
type II ribosome-inactivating protein | - |
Ricinus communis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in vitro assay at, substrate yeast ribosomes | Ricinus communis |
37 | - |
in vivo assay at, in Vero cells | Ricinus communis |