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3.2.2.22: rRNA N-glycosylase

This is an abbreviated version!
For detailed information about rRNA N-glycosylase, go to the full flat file.

Word Map on EC 3.2.2.22

Reaction

A4324 in 28S rRNA
+
H2O
=
deadenylated 28S rRNA
 +
adenine

Synonyms

(ribosome-inactivating protein)-like protein, abelesculin, ABRaA, abrin, abrin-a A chain, Abrus precatorius agglutinin, alpha-MC, alpha-momorcharin, alpha-pisavin, antiviral protein DAP-30, aralin, B-32, balsamin, BBAP1, BE27, beta-momorcharin, beta-pisavin, Bougainvillea xbuttiana antiviral protein 1, bouganin, camphorin, CAP30, cinnamomin, Cochinin B, cytotoxic ribosome-inactivating lectin, D6D54_07625, D6D54_08385, D6D54_08880, depurinating rRNA N-glycosidase, dianthin 30, dianthin 32, dianthin-30, diphtheria toxin, ebulin b, ebulitin alpha, ebulitin beta, ebulitin gamma, gelonin, gysophilin, hispin, IRAb, IRIP, Iris agglutinin b, Iris ribosome-inactivating protein, JIP60, karasurin-A, KML, lamjapin, luffaculin I, luffin P1, lychnin, MAP, marmorin, ME1, Mirabilis antiviral protein, mistletoe lectin I, mistletoe lectin II, mistletoe lectin III, ML-I, MLI, MLII, MLIII, MOD, modeccin, momorcochin, momorcochin-S, momordin, momordin I, momordin II, momorgrosvin, More, N-glycosidase, n-TCS, neo-trichosanthin, nigrin b, nigritin f1, nigritin f2, PAG, PAGase, PAP, PAP-I, PAP-II, PAP-III, PAP-R, PAP-S, PD-L, PD-L1, PD-L4, pepocin, PGase, pokeweed anti-viral protein, pokeweed antiviral protein, polynucleotide:adenosine glycosidase, polynucleotide:adenosine glycosylase, porrectin, RA, RCA-II, ribosomal inactivating protein, ribosomal ribonucleate N-glycosidase, ribosome inactivating protein, ribosome-inactivating protein, ribosome-inactivating protein 3, ribosome-inactivating protein type II, ribosome-inactivating proteins, ribosome-inactivating type II protein, ribosome-specific N-glycosidase, ricin, ricin A, ricin A chain, ricin A-chain, ricin toxin A-chain, ricin-A-chain, ricin-like protein, Ricinus communis agglutinin, Ricinus communis agglutinin II, RIP, RIP-1, RIP1, RIP2, RIPII, riproximin, RLP1, RLP2, RLP3, RLP4, RLP5, RLP6, RNA N-glycosidase, RNA-NGA, RNA-specific N-glycosidase, RNase Bo, Rpx, rRNA N-glycosidase, RTA, Sambucus nigra agglutinin, Sambucus nigra agglutinin I, SAP, saporin, saporin 6, saporin S-6, saporin S6, saporin-6, saporin-L1, saporin-S6, saporins, SBA, SCO7092, SEPIN, Shiga toxin, Shiga toxin 1, Shiga toxin 1 subunit A, Shiga toxin 2, Shiga toxin 2 subunit A, Shiga toxin type 1, Shiga toxin type 2, Shiga toxin-1, shiga-like toxin, shiga-like toxin I, sieboldin-b, single-chain ribosome-inactivating protein, SLT-I, SLT-II, SLT-IIv, SNA, SNA-I, SNA-V, SnaI, SNAIf, SNAV, SNLRP, SoRIP1, SoRIP2, stenodactylin, Stx, Stx type 1, Stx-1, Stx1, Stx1A, Stx2, Stx2A, Stx2dact, StxB, TCS, trichoanguin, trichomaglin, trichosanthin, Trichosanthrip, trichosnathin, TRIP, tritin-L, tritin-S, TYchi, type 1 ribosome inactivating protein, type 1 ribosome-inactivating protein, type 1 RIP, type 2 ribosome inactivating protein, type 2 ribosome-inactivating protein, type 2 RIP, type 2RIP, type 3 RIP, type I ribosome inactivating protein, type I ribosome-inactivating protein, type I RIP, type II ribosome inactivating protein, type II ribosome-inactivating protein, type II RIP, type III ribosome-inactivating protein, type III RIP, type-1 ribosome-inactivating protein, type-1 RIP, type-2 ribosome-inactivating protein, type-2 RIP, VAA-I, viscinum, Viscum album agglutinin I, viscumin, volkensin

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.2 Hydrolysing N-glycosyl compounds
                3.2.2.22 rRNA N-glycosylase

Cloned

Cloned on EC 3.2.2.22 - rRNA N-glycosylase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CAP30, DNA library construction, DNA and amino acid sequence determination and analysis, expression analysis after infection with tobacco mosaic virus or wounding, expression of His-tagged CAP30 in Escherichia coli strain M15 from two different expression vectors
-
cauliflower mosaic virus promoter
-
cDNA cloned and deduced amino acid sequence determined
-
cDNA cloned and transformed into Escherichia coli JM109
-
cloned and overexpressed in Escherichia coli BL21(DE3)
-
DNA and amino acid sequence determination and analysis, expression of MLI and MLIII B-subunits in Escherichia coli strain BL21(DE3)
-
DNA and amino acid sequence determination of PAP, expression in Escherichia coli strain JM101
DNA and amino acid sequence determination of PAP-II, expression in Escherichia coli strain JM101
DNA and amino acid sequence determination of PAP-R, expression in Escherichia coli strain JM101
DNA and amino acid sequence determination of PAP-S, expression in Escherichia coli strain JM101
Escherichia coli BL21 and TKB1
Escherichia coli strain DH5alpha with pDrive vector for cloning and strain TB1 with expression vector pMAL-c2X for expression
Escherichia coli XL-1 Blue (high transformation efficiency) and Bl21 (DE3) pLysS (high expression) with plasmid pET11d
-
expressed in Brassica juncea (Indian mustard)
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) pLysS
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli JM 101
expressed in Escherichia coli Rosetta(DE3) pLysS cells
expressed in Nicotiana tabacum protoplasts (cultivar Petit Havana SR1)
-
expressed in Saccharomyces cerevisiae strain W303
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae strain mak8-1 which is more resistant to the enzyme in vivo due to higher affinity for aminoacyl-tRNA
-
expression of A chain in Escherichia coli with His-tag. Construction of replication-deficient ricin B chain adenovirus-green fluorescent protein fusion protein
-
expression of both the A and the B chains of KML separately in a bacterial expression system
-
expression of His-tagged wild-type and mutant PAPs in Escherichia coli strain BL21(DE3)
expression of His6-tagged ME1 in Escherichia coli strain BL21(DE3) for which ME1 is nontoxic
expression of His6-tagged optimized abricin A-chain in Escherichia coli strain BL21(DE3)
expression of IRAb in transgenic Nicotiana tabacum cv. Samsun plant leaves
Iris sp.
-
expression of IRIP in transgenic Nicotiana tabacum cv. Samsun plants
Iris sp.
-
expression of isozymes in Escherichia coli strain BL21(DE3). Synthesis and expression of a PD-L1 synthetic gene in Escherichia coli strain BL21(DE3)
expression of precursor form of ricin A chain in Saccharomyces cerevisiae
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expression of ricin A chain in Saccharomyces cerevisiae
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expression of ricin B-subunit in Nicotiana tabacum hairy roots, the recombinant protein is secreted to the culture medium
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expression of RTA and each of the six RLPs in polyhistidine-tagged form in Escherichia coli BL21-AI cells
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expression of SNA-I, SNA-IV, SNA-V, and SNLRP in Nicotiana tabacum cv. Samsun NN transgenic plants, expression levels, overview
-
expression of wild-type and mutant karasurin-A in Escherichia coli as maltose-binding protein fusion proteins
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expression of wild-type and mutant ricin A chains in Escherichia coli strain JM101
-
expression of wild-type and mutant RTAs in Escherichia coli strain JM101
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expression of wild-type PAP in yeast, and of mutant PAPs in Escherichia coli
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functional expression of CBD-intein-Gel fusion protein in Escherichia coli strain Bl21(DE3) in inclusion bodies
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fusion gene of enhanced green fluorescent protein and ricin A chain, expression in Escherichia coli
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fusion protein of a 90-amino acid peptide from the simian rotavirus SA-11 nonstructural protein, NSP4 linked to the N-terminus of the ricin B subunit protein, expression in Escherichia coli
-
gene RIP3:1 is expressed under control of the Opaque-2 promoter
gene RIP3:2, genomic DNA library screening, DNA and amino acid sequence determination and analyis, RNA mapping and expression profiling, expression is not under control of the Opaque-2 promoter, expression of gene RIP3:2 encoding isozyme RIP2 in Escherichia coli strain BL21(DE3) as N-terminally His-tagged protein
gene ripsc, DNA and amino acid sequence determination and analysis, subcloning in Escherichia coli strain TG1, functional expression of Hi6-tagged enzyme in Escherichia coli strain BL21(DE3) as recombinant protein of about 30 kDa in inclusion bodies. Expression in Streptomyces lividans and Escherichia coli, RIPsc overproduction results in dramatic decrease of the growth rate. In addition, intracellular production is deleterious for Saccharomyces cerevisiae. Detailed overview
-
isozyme MLIII, DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, expression of the B-subunit in Escherichia coli strain BL21(DE3) in inclusion bodies
-
MAP30 cloned and expressed in Escherichia coli
-
nucleotide sequence of cDNA of cinnamomin A- and B-chains, transformed into Escherichia coli DH12S
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overexpression of ricin A-chain RTA V81M without N- and C-terminal extensions in Escherichia coli strain BL21(DE3)
-
plasmid pMAL-c2 to amplify pokeweed antiviral protein and karasurin-A by PCR, expression in Escherichia coli XL1-Blue cells with expression plasmid pGEX-6P-1
potato plants expressing antiviral protein exhibits resistance against potato virus X, potato virus Y, and potato leafroll virus
-
recombinant proteins expressed in Escherichia coli BL21
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RTA gene from genomic DANN cloned and expressed in Escherichia coli XL-1 blue
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separate expression of A and B chain in Escherichia coli
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SoRIP1 and SoRIP2, DNA and amino acid sequence determination and analysis, phylogenetic analysis
Stx2 gene, DNA and amino acid sequence determination, genotyping of 117 lineage I, I/II, and II E. coli O157:H7 strains
synthetic gene variants expressed in Escherichia coli MM294
-
the enzymatically active ricin A chain is expressed in Escherichia coli BL21(DE3)pLysS cells
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transgenic tobacco expressing trichosanthin is completely resistant against infection by turnip mosaic virus
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