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Literature summary for 3.2.2.22 extracted from

  • Yoshinari, S.; Koresawa, S.; Yokota, S.; Sawamoto, H.; Tamura, M.; Endo, Y.
    Gypsophilin, a new type 1 ribosome-inactivating protein from Gypsophila elegans: purification, enzymic characterization, and subcellular localization (1997), Biosci. Biotechnol. Biochem., 61, 324-331.
    View publication on PubMed
Search for more literature for 3.2.2.22

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm distributed within vacuoles Gypsophila elegans 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 gel filtration Gypsophila elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
rRNA + H2O Gypsophila elegans
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Gypsophila elegans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Gypsophila elegans

Source Tissue

Source Tissue Comment Organism Textmining
leaf intercellular space Gypsophila elegans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 specific activity 17941000 units/mg, 1 unit is the amount that will cause 50% depurination in 0.05 ml Gypsophila elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
rRNA + H2O
-
 Gypsophila elegans ?
-
 ?
rRNA + H2O cleaves the N-glycosidic bond and removes adenine at A-4324 in 28 S rRNA when intact rat ribosomes are the substrate, cleaves the same N-glycosidic bond in naked 28 S rRNA Gypsophila elegans ?
-
 ?

Synonyms

Synonyms Comment Organism
gysophilin
-
 Gypsophila elegans

pI Value

Organism Comment pI Value Maximum pI Value
Gypsophila elegans isoelectric focusing
-
 10.1