Information on EC 3.2.2.22 - rRNA N-glycosylase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.2.22
-
RECOMMENDED NAME
GeneOntology No.
rRNA N-glycosylase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of the N-glycosylic bond at A-4324 in 28S rRNA from rat ribosomes
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
hydrolysis of N-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
rRNA N-glycohydrolase
Ricin A-chain and related toxins show this activity. Naked rRNA is attacked more slowly than rRNA in intact ribosomes. Naked rRNA from Escherichia coli is cleaved at a corresponding position.
CAS REGISTRY NUMBER
COMMENTARY hide
113756-12-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
abelesculin
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Manually annotated by BRENDA team
pectin methylesterase has ribosome inactivating protein activity
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
hairy melon, var. chieh-qua
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
camphor tree
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Manually annotated by BRENDA team
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UniProt
Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
mushroom, comparison with ribosome inhibitory proteins of bitter gourd, hairy gourd, ridge gourd, garden pea, Flammulina velutipes (mushroom with velutin)
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Manually annotated by BRENDA team
Iris sp.
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
no activity in Arabidopsis thaliana
does not express detectable amounts of RIPs or contain a sequence encoding a putative RIP
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Manually annotated by BRENDA team
garden pea, var. arvense Poir
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Manually annotated by BRENDA team
kelp, cryptogamic marine algal plant, A
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
japanese elderberry
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Manually annotated by BRENDA team
Luo Han Guo meaning monk's fruit, also known as Siratia grosvenorii
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Manually annotated by BRENDA team
isozyme SoRIP1
UniProt
Manually annotated by BRENDA team
gene ripsc
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Manually annotated by BRENDA team
snake gourds
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Manually annotated by BRENDA team
Trichosanthes lepiniate
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
additional information
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the toxicity of RIPs to cells and consequently to animals is highly variable and depends on their structure: in general, type 1 RIPs have a lower toxicity than type 2 RIPs
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
14-mer rRNA with GAGA tetraloop + H2O
14-mer rRNA with GabGA + ?
show the reaction diagram
-
substrate and product with specific DNA loop probe, fluorophore and quencher, 37°C, citrate buffer, pH 4.0, than neutralization to pH 7.6 and hybridization with DNA-probes
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?
2'-anthraniloyl 7-methylguanosine triphosphate + H2O
?
show the reaction diagram
-
depurination downstream of cap structure, 2.4-fold enhanced binding to m7GTP upon the addition of translation initiation factor G4 (elFiso4G)
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?
23S rRNA + H2O
?
show the reaction diagram
25S rNA + H2O
?
show the reaction diagram
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enzyme depurinates the sarcin/ricin loop when the A-site of the ribosomal peptidyl-transferase center is unoccupied
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?
25S rRNA + H2O
?
show the reaction diagram
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depurination
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?
26S rRNA + H2O
?
show the reaction diagram
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depurination of ribosomes from yeast, product determination using aniline coupling
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?
28S RNA + H2O
?
show the reaction diagram
substrate from rat liver ribosomes, depurination of A4324, generation of a 420nt fragment, product determination using aniline coupling
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?
28S rRNA + H2O
28S rRNA fragment + ?
show the reaction diagram
28S rRNA + H2O
400-bp Endo fragment + ?
show the reaction diagram
-
-
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?
28S rRNA + H2O
?
show the reaction diagram
28S rRNA + H2O
apurinic 28S rRNA + adenine
show the reaction diagram
28S rRNA + H2O
Endo's fragment + ?
show the reaction diagram
-
-
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?
28Sdelta rRNA + H2O
?
show the reaction diagram
5'-dG1dC2dG3dC4dG5A6dG7dA8dG9dC10dG11dC12-3' + H2O
?
show the reaction diagram
-
GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-dG1dC2dG3dC4dG5dA6dG7A8dG9dC10dG11dC12-3' + H2O
?
show the reaction diagram
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GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-dG1dC2dG3dC4dG5dA6G7dA8dG9dC10dG11dC12-3' + H2O
?
show the reaction diagram
-
GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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-
?
5'-dG1dC2dG3dC4G5dA6dG7dA8dG9dC10dG11dC12-3' + H2O
?
show the reaction diagram
-
GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-G1C2G3C4dG5A6dG7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
-
GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-G1C2G3C4dG5A6G7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
-
GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-G1C2G3C4dG5A6G7dA8G9C10G11C12-3' + H2O
?
show the reaction diagram
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GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-G1C2G3C4G5A6dG7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
-
GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-G1C2G3C4G5A6G7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
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GAGA stem-loop RNA substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, preferred substrates to DNA stem loop derivatives, overview
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?
5'-G1C2G3C4G5A6G7dA8G9C10G11C12-3' + H2O
?
show the reaction diagram
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GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
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?
5'-G1C2G3C4G5A6G7mA8G9C10G11C12-3' + H2O
?
show the reaction diagram
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RNA-2'-methoxy nucleic acid hybrid stem-loop substrate
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?
5'-G1C2G3C4G5A6mG7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
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RNA-2'-methoxy nucleic acid hybrid stem-loop substrate
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?
5'-G1C2G3C4G5dA6G7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
-
GAGA stem-loop RNA-DNA hybrid substrate, analysis of activity with substrate derivative possessing variations in the tetraloop G5-A8, overview
-
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?
5'-G1C2G3C4G5mA6G7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
-
RNA-2'-methoxy nucleic acid hybrid stem-loop substrate
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?
5'-G1C2G3C4mG5A6G7A8G9C10G11C12-3' + H2O
?
show the reaction diagram
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RNA-2'-methoxy nucleic acid hybrid stem-loop substrate
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?
5'/biotin/AGCGGGAGAGdAAAUCUCCC + H2O
5'/biotin/AGCGGGAGAG + ?
show the reaction diagram
5'/biotin/AGCGGGAGAGdAGAUCUCCC + H2O
5'/biotin/AGCGGGAGAG + ?
show the reaction diagram
5'/biotin/AGCGGGAGAGdUGAUCUCCC + H2O
5'/biotin/AGCGGGAGAG + ?
show the reaction diagram
8-vinyl-2'-deoxyadenosine containing 10-mer stem-tetraloop RNA + H2O
8-vinyladenine + ?
show the reaction diagram
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slow
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?
A-10 RNA + H2O
?
show the reaction diagram
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i.e. 5'-CGCGAGAGCG-3'
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?
A-14 2-dA stem-loop RNA/DNA hybrid + H2O
?
show the reaction diagram
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?
adenosine + H2O
adenine + D-ribose
show the reaction diagram
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holoenzyme is active against small substrates
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?
adenosine in rRNA + H2O
deadenylated rRNA + adenine
show the reaction diagram
Artemia sp. ribosome + H2O
?
show the reaction diagram
-
depurination of rRNA
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?
brome mosaic virus RNA + H2O
?
show the reaction diagram
cyclic dG-(N-benzyl-aza-deoxyribosyl)-dGdA + H2O
?
show the reaction diagram
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?
cyclic dGdAdGdA + H2O
?
show the reaction diagram
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?
cyclic GdAGA + H2O
?
show the reaction diagram
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?
cyclic oxime GAGA + H2O
?
show the reaction diagram
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?
cyclic phosphorothioyl dGdAdGdA + H2O
?
show the reaction diagram
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ring closure via a phosphorothioate bond
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?
dGdAdGdA + H2O
?
show the reaction diagram
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?
dGdCdGdCdGdAdGdAdGdCdGdC + H2O
?
show the reaction diagram
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?
DNA + H2O
?
show the reaction diagram
GCGCGAGAGCGC + H2O
GCGCGGAGCGC + ?
show the reaction diagram
-
DNA substrate (100 pmol/microl) mimicking natural rRNA substrate, reaction buffer: 10 mM ammonium citrate with 1 mM ethylenediaminetetraacetic acid, pH 4, 37°C, 4 h
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GdAGA + H2O
?
show the reaction diagram
hering sperm DNA + H2O
?
show the reaction diagram
-
adenine releasing activity
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?
hsDNA + H2O
?
show the reaction diagram
-
-
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?
human mtDNA + H2O
?
show the reaction diagram
-
saporin 6 shows specific nuclease activity towards the D-loop of human mitochondrial, covalently closed circular DNA, determination of sites of action by restriction analysis
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?
large RNA + H2O
?
show the reaction diagram
removal of a single adenine
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?
large rRNA + H2O
?
show the reaction diagram
large rRNA + H2O
large rRNA fragments, partially deadenylated
show the reaction diagram
substrate are intact rabbit ribosomes, best substrate, or ribosomes from Aspergillus flavus, low activity, or Zea mays, the latter are poor substrates, depurination of specific sites in rRNA, formation of RNA fragments
fragment size overview, product determination using aniline coupling
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?
linear GAGA + H2O
?
show the reaction diagram
-
-
-
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?
mRNA + H2O
?
show the reaction diagram
-
in vitro translation inhibition assay in rabbit reticulocyte lysate, 30 degrees Celsius
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?
mRNA cap + H2O
?
show the reaction diagram
-
mRNA cap structures including N7-methyl guanine, N7-methyl guanosine diphosphate, and N7-methyl guanosine triphosphate bind to RIP-1
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?
naked rRNA + H2O
?
show the reaction diagram
non-ribosomal RNA + H2O
?
show the reaction diagram
-
e.g. mRNA: Phytophthora cryptotogea B26 RNA, or Arabidopsis thaliana RNA, single-stranded, identification of cleavage sites, overview
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?
pGEM-4Z plasmid DNA + H2O
?
show the reaction diagram
-
isozyme saporin-SO6 wrappes the DNA and localizes in a variable manner along the DNA molecule to perform fragmentalized digestion
-
-
ir
poly(A) + H2O
?
show the reaction diagram
-
-
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?
poly(A) + H2O
adenine + ?
show the reaction diagram
-
-
-
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?
poly(A) substrate + H2O
?
show the reaction diagram
-
-
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?
polyA + H2O
?
show the reaction diagram
rabbit reticulocyte 80S rRNA + H2O
?
show the reaction diagram
-
-
-
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?
rabbit ribosome 80S rRNA + H2O
?
show the reaction diagram
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good substrate for saporin-L1, low activity by saporin-S6
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?
rat ribosome + H2O
?
show the reaction diagram
-
depurination of rRNA
-
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?
ribosome + H2O
?
show the reaction diagram
-
inhibition of protein synthesis in rabbit reticulocyte lysate by depurination of rRNA
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?
ribosomes + H2O
?
show the reaction diagram
-
substrate from yeast
-
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?
RNA + H2O
?
show the reaction diagram
rRNA + H2O
?
show the reaction diagram
sarcin/ricin domains of Escherichia coli 23S rRNA + H2O
?
show the reaction diagram
-
-
-
-
?
sarcin/ricin domains of Rattus norvegicus 28S rRNA + H2O
?
show the reaction diagram
-
-
-
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?
single-stranded DNA + H2O
?
show the reaction diagram
-
at 90C heat-denatured substrate
-
-
?
stem-loop substrate A-10 + H2O
?
show the reaction diagram
-
-
-
-
?
supercoiled DNA + H2O
?
show the reaction diagram
-
isozyme saporin-SO6 cleaves the supercoiled DNA resulting in linearized and relaxed DNA forms
-
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?
supercoiled double-stranded DNA + H2O
nicked and linear forms of DNA
show the reaction diagram
tobacco mosaic virus RNA + H2O
?
show the reaction diagram
tRNA + H2O
?
show the reaction diagram
-
adenine releasing activity
-
-
?
un-capped RNA + H2O
?
show the reaction diagram
-
-
-
-
?
yeast 60S rRNA + H2O
?
show the reaction diagram
-
-
-
-
?
yeast ribosome + H2O
?
show the reaction diagram
-
depurination of rRNA
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
23S rRNA + H2O
?
show the reaction diagram
-
-
-
-
?
28S rRNA + H2O
28S rRNA fragment + ?
show the reaction diagram
28S rRNA + H2O
?
show the reaction diagram
28S rRNA + H2O
apurinic 28S rRNA + adenine
show the reaction diagram
28S rRNA + H2O
Endo's fragment + ?
show the reaction diagram
P16094
-
-
-
?
adenosine in rRNA + H2O
deadenylated rRNA + adenine
show the reaction diagram
-
rRNA is depurinated thus producing inactive ribosomes and inhibition protein synthesis probably due to the lack of the interaction between depurinated ribosomes and elongation factor 2
-
-
?
brome mosaic virus RNA + H2O
?
show the reaction diagram
-
depurination of the viral RNA, anti-viral activity of PAP by inhbition of accumulation of viral RNA and viral proliferation in barley protoplasts, overview
-
-
?
large rRNA + H2O
?
show the reaction diagram
RNA + H2O
?
show the reaction diagram
-
the enzyme is active on viral RNA, e.g. from Human immunodeficiency virus-1 and Brome mosaic virus, RNA accession mechanism via interaction with translation initiation factors 4G and iso4G, overview
-
-
?
rRNA + H2O
?
show the reaction diagram
tobacco mosaic virus RNA + H2O
?
show the reaction diagram
-
SNA-I, SNA-V, and SNLRP show anti-viral activity
-
-
?
additional information
?
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