Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

3.2.1.7: inulinase

This is an abbreviated version!
For detailed information about inulinase, go to the full flat file.

Word Map on EC 3.2.1.7

Reaction

(Frufbeta(2-1))n
+
H2O
=
(Frufbeta(2-1))n-m
 +
(Frufbeta(2-1))m

Synonyms

2,1-beta-D-fructanfructanohydrolase, AARAC_000847, An11g03200, AUD_0597, beta-2,1-D-fructan fructanohydrolase, beta-D-fructan fructanohydrolase, Endo I, Endo-I, Endo-II, endo-inulinase, endoinulinase, EnIA, extracellular endo-inulinase, fructofuranosyl hydrolase, Fructozyme L, Insulinase, inu1, Inu2, InuA, inuB, inuC, inuE, inulase, inulinase, P-III, PENSUB_1400, PENSUB_5768, PENSUB_5772, TCE0_044r16122, TSTA_051680, Xcp KM 24

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.7 inulinase

Purification

Purification on EC 3.2.1.7 - inulinase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation
-
ammonium sulfate precipitation, gel filtration on Sephadex G-100
-
by ammonium sulfate precipitation, DEAE-Sephacel column and phenyl-Sepharose column, 28.7fold
-
by DEAE-Trisacryl Plus column and Superose column
-
by metal-affinity chromatography
-
endoinulinase I, II and III
-
enzyme Xcp KM 24 to homogeneity 1.74fold by ammonium sulfate fractionation and gel filtration
-
from the extracellular extract, by gel filtration, 31.4fold purified
-
immobilized on Ca-alginate beads
-
immobilized onto Amberlite IRC 50
-
recombinant enzyme
-
recombinant enzyme from Yarrowia lipolytica strain ENop56 by dialysis and anion exchange chromatography, the enzyme is then deglycosylated by endoglycosidase Endo H, followed by gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant MBP-tagged enzyme from Escherichia coli strain BL21 by amylose affinity chromatography, and gel filtration
recombinant protein
-
the commercial preparation of endoinulase is further purified 8.2fold by anion exchange chromatography to exclude any exoinulase activity
wild-type and mutant M-30