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3.2.1.7: inulinase

This is an abbreviated version!
For detailed information about inulinase, go to the full flat file.

Word Map on EC 3.2.1.7

Reaction

(Frufbeta(2-1))n
+
H2O
=
(Frufbeta(2-1))n-m
 +
(Frufbeta(2-1))m

Synonyms

2,1-beta-D-fructanfructanohydrolase, AARAC_000847, An11g03200, AUD_0597, beta-2,1-D-fructan fructanohydrolase, beta-D-fructan fructanohydrolase, Endo I, Endo-I, Endo-II, endo-inulinase, endoinulinase, EnIA, extracellular endo-inulinase, fructofuranosyl hydrolase, Fructozyme L, Insulinase, inu1, Inu2, InuA, inuB, inuC, inuE, inulase, inulinase, P-III, PENSUB_1400, PENSUB_5768, PENSUB_5772, TCE0_044r16122, TSTA_051680, Xcp KM 24

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.7 inulinase

Cloned

Cloned on EC 3.2.1.7 - inulinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, recombinant expression of MBP-tagged enzyme in Escherichia coli strain BL21
DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of the codon-optimized gene in Pichia pastoris strain KM71, method optimization, overview
expresion in Penicillium canescens
-
expressed on the cell surface of Saccharomyces cerevisiae EBY100 cells
-
expression in Escherichia coli
-
expression in Pichia pastoris
gene inu1, DNA and amino acid sequence determination and analysis, domain structure, phylogenetic analysis, recombinant expression of full-length and truncated enzymes in Pichia pastoris
gene inuA, recombinant overexpresssion in Yarrowia lipolytica strain Enop56 (engineered from strain Po1h), during fermentation with an inulin concentration of 600 g/l in a 10-l bioreactor, the inulinase activity, fructooligosaccharides (FOS) titer, yield, and productivity reach 551.6 U/ml, 546.6 g/l, 0.91 gFOS/g Inulin, and 15.18 g/l/h, respectively
INU2 gene encoding endoinulinase expressed in SUC2-deleted Saccharomyces cerevisiae
inulinase gene without the signal sequence subcloned into pPICZaA expression vector and expressed in Pichia pastoris X-33
phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
wild-type and mutant M-30 PCR products ligated into pGEM-T easy vector and transformed into the competent cells of Escherichia coli JM109