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Literature summary for 3.2.1.7 extracted from

  • Kushi, R.T.; Monti, R.; Contiero, J.
    Production, purification and characterization of an extracellular inulinase from Kluyveromyces marxianus var. bulgaricus (2000), J. Ind. Microbiol. Biotechnol., 25, 63-69.
No PubMed abstract available
Search for more literature for 3.2.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 4.58 mg/ml, for sucrose Kluyveromyces marxianus
additional information
-
 additional information 7.41 mg/ml, for raffinose Kluyveromyces marxianus
additional information
-
 additional information 86.9 mg/ml, for inulin Kluyveromyces marxianus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Kluyveromyces marxianus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Kluyveromyces marxianus
77000
-
 gel filtration on Superose column Kluyveromyces marxianus

Organism

Organism UniProt Comment Textmining
Kluyveromyces marxianus
-
 var. bulgaricus
-

Purification (Commentary)

Purification (Comment) Organism
by DEAE-Trisacryl Plus column and Superose column Kluyveromyces marxianus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
 Kluyveromyces marxianus beta-D-fructose + inulobiose + fructooligosaccharides
-
 ?
raffinose + H2O
-
 Kluyveromyces marxianus D-fructose + D-galactopyranosyl-(1-6)-D-glucopyranoside
-
 ?
sucrose + H2O
-
 Kluyveromyces marxianus beta-D-fructose + D-glucose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Kluyveromyces marxianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.7
-
 for sucrose Kluyveromyces marxianus