3.2.1.7: inulinase
This is an abbreviated version!
For detailed information about inulinase, go to the full flat file.
Word Map on EC 3.2.1.7
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3.2.1.7
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fructose
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kluyveromyces
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aspergillus
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marxianus
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niger
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jerusalem
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artichoke
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invertase
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syrup
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fructans
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fructooligosaccharides
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nutrition
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food industry
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chicory
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ficuum
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saccharification
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beta-fructofuranosidase
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guilliermondii
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plackett-burman
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synthesis
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levanase
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high-fructose
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agave
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industry
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beta-fructosidase
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tuberosus
- 3.2.1.7
- fructose
- kluyveromyces
- aspergillus
- marxianus
- niger
-
jerusalem
- artichoke
- invertase
- syrup
- fructans
- fructooligosaccharides
- nutrition
- food industry
- chicory
- ficuum
-
saccharification
- beta-fructofuranosidase
- guilliermondii
-
plackett-burman
- synthesis
- levanase
-
high-fructose
- agave
- industry
- beta-fructosidase
- tuberosus
Reaction
Synonyms
2,1-beta-D-fructanfructanohydrolase, AARAC_000847, An11g03200, AUD_0597, beta-2,1-D-fructan fructanohydrolase, beta-D-fructan fructanohydrolase, Endo I, Endo-I, Endo-II, endo-inulinase, endoinulinase, EnIA, extracellular endo-inulinase, fructofuranosyl hydrolase, Fructozyme L, Insulinase, inu1, Inu2, InuA, inuB, inuC, inuE, inulase, inulinase, P-III, PENSUB_1400, PENSUB_5768, PENSUB_5772, TCE0_044r16122, TSTA_051680, Xcp KM 24
ECTree
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Activating Compound
Activating Compound on EC 3.2.1.7 - inulinase
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inulin
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with raw inulin in bioreactor, progressive increase in inulinase production with increase in inulin concentration up to 4.0% with a maximum inulinase activity of 50.2 IU/ml
Sodium dodecyl sulfate
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0.001% is most effective for the inulinase activity (36.9 IU/ml)
Triton X-100
activates the wild-type enzyme by 33% at 0.1% v/v
the fungus shows good growth on a medium containing 40% (v/v) of dandelion tap root extract composed of 50 g tap roots blended with 200 ml water and 2% yeast extract medium and produces 55 U/ml of inulinase activity in 96 h at 30°C and 150 rpm during the liquid fermentation. Dandelion tap root extract in the liquid medium induces the endoinulinase synthesis
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additional information
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inulinase yield in bioreactor is almost 6times higher than basal medium used in shake flask. Tween-60 has no significant effect. Inoculum of 12 h old yeast culture is optimum for inulinase activity (36.9 IU/ml). 5% of 12 h old culture is optimum for the production of inulinase in the medium. Progressive increase in inulinase activity up to 37.0 IU/ml as the agitation speed is increased to 150 rpm. Maximum production of inulinase (40.2 IU/ml) after 60 h of incubation
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additional information
maximum inulinase production (55.4 U/ml) by strain YS-1 is obtained at an agitation rate of 200 rpm and aeration of 0.75 volume/volume per minute in a stirred tank reactor (1.5 l) with a fermentation time of 60 h or inulinase activity of 50.2 U/ml is obtained from the same yeast strain under agitation (200 rpm) and aeration (0.75 volume/volume per minute) at 30°C after 60 h of fermentation in 1.5 l of fermentor when raw inulin (4.0%) from root tubers of Asparagus officinalis is used as the production medium. When strain NRRL Y-7571 is grown in the culture containing sugarcane bagasse as support and carbon source and corn steep liquor as nitrogen supplement, the extracellular inulinase concentration reaches 391.9 U/g of dry fermented bagasse. The use of soybean bran decreases the time to reach the maximum activity from 96 to 24 h and the maximum productivity achieved strain NRRL Y-7571 is 8.87 U/gds per h
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additional information
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high endoinulinase productivity of 9.9 U/ml when grown in a liquid medium containing inulin as the carbon source at 30°C for 4 days
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additional information
no effect by 1 mM DTT on wild-type enzyme activity
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additional information
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no effect by 1 mM DTT on wild-type enzyme activity
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