Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.7 extracted from

  • Onodera, S.; Shiomi, N.
    Purification and substrate specificity of endo-type inulinase from Penicillium purpurogenum (1988), Agric. Biol. Chem., 52, 2569-2576.
No PubMed abstract available
Search for more literature for 3.2.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.21
-
 inulin DP = 35 Talaromyces purpureogenus
0.24
-
 (fructosylsucrose)9
-
 Talaromyces purpureogenus
0.33
-
 (fructosylsucrose)7
-
 Talaromyces purpureogenus
0.85
-
 (fructosylsucrose)6
-
 Talaromyces purpureogenus
2.8
-
 (fructosylsucrose)4
-
 Talaromyces purpureogenus
3.8
-
 (fructosylsucrose)5
-
 Talaromyces purpureogenus
8.4
-
 1-kestose
-
 Talaromyces purpureogenus
16
-
 nystose
-
 Talaromyces purpureogenus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
 gel filtration Talaromyces purpureogenus

Organism

Organism UniProt Comment Textmining
Talaromyces purpureogenus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Talaromyces purpureogenus

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Talaromyces purpureogenus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(fructosylsucrose)4 + H2O
-
 Talaromyces purpureogenus ?
-
 ?
(fructosylsucrose)5 + H2O
-
 Talaromyces purpureogenus ?
-
 ?
(fructosylsucrose)6 + H2O
-
 Talaromyces purpureogenus ?
-
 ?
(fructosylsucrose)7 + H2O
-
 Talaromyces purpureogenus ?
-
 ?
(fructosylsucrose)9 + H2O
-
 Talaromyces purpureogenus ?
-
 ?
1-kestose + H2O
-
 Talaromyces purpureogenus ?
-
 ?
fructooligosaccharide + H2O (fructosylsucrose)4 to (fructosylsucrose)9 Talaromyces purpureogenus ?
-
 ?
inulin + H2O
-
 Talaromyces purpureogenus oligofructosides the major products are trisaccharides, tetrasaccharides, pentasaccharides and hexasaccharides ?
additional information no activity with sucrose Talaromyces purpureogenus ?
-
 ?
nystose + H2O
-
 Talaromyces purpureogenus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 64000, SDS-PAGE Talaromyces purpureogenus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 pH 5.1, 10 min, stable up to Talaromyces purpureogenus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.1
-
-
 Talaromyces purpureogenus

pH Range

pH Minimum pH Maximum Comment Organism
4.3 6.5 pH 4.3: about 40% of maximal activity at pH 4.3 and at pH 6.5 Talaromyces purpureogenus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7.5 4°C, 24 h, stable Talaromyces purpureogenus