Application | Comment | Organism |
---|---|---|
industry | inulinase activity (115.0 U/ml) in liquid production medium by the mutant M-30 is the highest reported so far. Inulinase produced by the mutant M-30 of the marine yeast strain has potentially for use in biotechnological fields | Meyerozyma guilliermondii |
Cloned (Comment) | Organism |
---|---|
wild-type and mutant M-30 PCR products ligated into pGEM-T easy vector and transformed into the competent cells of Escherichia coli JM109 | Meyerozyma guilliermondii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutants M-30 (115.0 U/ml) and M-31 (52.6 U/ml) show improved inulinase production compared to the wild-type. Initial moisture, inoculum, amount ratio of wheat bran to rice bran, temperature, pH for the maximum inulinase production by the mutant M-30 is 60.5%, 2.5%, 0.42, 30°C and 6.50, respectively. Under the optimized conditions, 455.9 U/grams of dry substrate (gds) of inulinase activity is reached in the solid state fermentation culture of the mutant. Glucose repression on the inulinase production by the mutant M-30 is relieved in some degree compared to that in its parent strain when the added glucose concentrations in the media are higher than 2.0% | Meyerozyma guilliermondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | glucose represses inulinase production | Meyerozyma guilliermondii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meyerozyma guilliermondii | A5DHM6 | - |
- |
Meyerozyma guilliermondii 1 | A5DHM6 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and mutant M-30 | Meyerozyma guilliermondii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
inulin + H2O | - |
Meyerozyma guilliermondii | ? | - |
? | |
inulin + H2O | - |
Meyerozyma guilliermondii 1 | ? | - |
? |