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Literature summary for 3.2.1.7 extracted from

  • Nakamura, T.; Shitara, A.; Matsulda, S.; Matsuo, T.; Suiko, M.; Ohta, K.
    Production, purification and properties of an endoinulinase of Penicillium sp. TN-88 that liberates inulotriose (1997), J. Ferment. Bioeng., 84, 313-318.
No PubMed abstract available
Search for more literature for 3.2.1.7

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Penicillium sp.
carbodiimide
-
 Penicillium sp.
EDTA
-
 Penicillium sp.
Fe3+
-
 Penicillium sp.
Hg2+
-
 Penicillium sp.
N-bromosuccinimide
-
 Penicillium sp.
PCMB
-
 Penicillium sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 inulin at 40°C, pH 5.0 Penicillium sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ stimulates Penicillium sp.

Organism

Organism UniProt Comment Textmining
Penicillium sp.
-
 TN-88
-
Penicillium sp. TN-88
-
 TN-88
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 1.4% carbohydrate Penicillium sp.

Purification (Commentary)

Purification (Comment) Organism
-
 Penicillium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
105
-
-
 Penicillium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
inulin + H2O
-
 Penicillium sp. oligofructosides inulotriose and inulotetraose are liberated as the main products after 24 h, prolonged reaction yields inulotriose as the main product ?
inulin + H2O
-
 Penicillium sp. TN-88 oligofructosides inulotriose and inulotetraose are liberated as the main products after 24 h, prolonged reaction yields inulotriose as the main product ?
additional information no activity with levan Penicillium sp. ?
-
 ?
additional information no activity with sucrose Penicillium sp. ?
-
 ?
additional information no activity with raffinose Penicillium sp. ?
-
 ?
additional information no activity with levan Penicillium sp. TN-88 ?
-
 ?
additional information no activity with sucrose Penicillium sp. TN-88 ?
-
 ?
additional information no activity with raffinose Penicillium sp. TN-88 ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 30 min, stable Penicillium sp.
60
-
 30 min, complete inactivation Penicillium sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
 Penicillium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 7 24 h, stable Penicillium sp.