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3.2.1.37: xylan 1,4-beta-xylosidase

This is an abbreviated version!
For detailed information about xylan 1,4-beta-xylosidase, go to the full flat file.

Word Map on EC 3.2.1.37

Reaction

(Xylbeta(1-4))x
+
H2O
=
beta-D-xylopyranose
 +
(Xylbeta(1-4))x-1

Synonyms

1,4-beta-D-xylan xylohydrolase, 1,4-beta-D-xylosidase, 1.4-beta-D-xylanase, exo-type, ABF3, AbfA, AbfB, AbXyl, alpha-arabinofuranosidase/beta-xylosidase, alpha-L-arabinofuranosidase, alpha-L-arabinofuranosidase 3, alpha-L-arabinofuranoside arabinofuranohydrolase, arabinofuranosidase 3, AXH-d3, Axy43A, BcXyl, beta-1,4-xylosidase, beta-D-xylopyranosidase, beta-D-xylosidase, beta-D-xylosidase/alpha-L-arabinofuranosidase, beta-D-xyloside xylohydrolase, beta-glucosidase/xylosidase, beta-xylosidase, beta-xylosidase A, beta-xylosidase B, beta-xylosidase I, beta-xylosidase II, beta-xylosidase-1, beta-xylosidase-2, beta-xylosidase-3, beta-xylosidase-4, betaXTE, betaXTR, bifunctional beta-glucosidase/xylosidase, BlxA, Bxl1, BxTW1, Bxyl, ccxyl3a, EcXyl43 beta-xylosidase, exo-1,4-beta-D-xylosidase, exo-1,4-beta-xylosidase, Exo-beta-(1,4)-xylanase, GH43 beta-1,4-xylosidase, GH43 xylosidase, GH43B6, glycoside hydrolase family 3 beta-xylosidase, More, Pae1263, RrXyl43A, Rubg3A, Rubg3B, RuBGX1, RuXyn1, SSO3032, Sxa, Thxyl43A, Tpexyl3, X12345, Xln-DT, Xyl, Xyl I, XYL1, Xyl3A, XYL4, xyl43, xyl43A, Xyl43C, Xyl5, XYLA, Xylan 1,4-beta-xylosidase, xylanase, XylB, XylC, XylCD2, xylobiase, xylosidase, XynB, XynB2, XynB3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.37 xylan 1,4-beta-xylosidase

Inhibitors

Inhibitors on EC 3.2.1.37 - xylan 1,4-beta-xylosidase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,3-bis[tris(hydroxymethyl)methylamino]propane
-
-
1-amino-2-propanol
-
-
1-aminopropane
-
-
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide
-
strong inhibition, 69% residual activity at 200 mM
1-ethyl-3-(3-dimethylaminopropyl)carbodiimide
-
-
2,3-epoxypropyl beta-D-xylobioside
-
irreversible inactivation
2-hydroxypropane
-
-
2-mercaptoethanol
2-propanol
-
-
4-Aminobenzoate
1 mM, 20% inhibition
4-Aminobenzoic acid
about 90% residual activity at 10 mM
4-methylumbelliferyl-alpha-L-arabinofuranoside
substrate inhibition
4-methylumbelliferyl-beta-D-xylopyranoside
substrate inhibition
4-nitrophenyl-beta-D-xylopyranoside
5,5'-dithiobis(2-nitrobenzoic acid)
-
rapid inactivation by modification of an essential thiol group
5-bromosuccinimide
-
strong inhibition, 84.7% complete inhibition at 0.1 mM
AgNO3
1 mM, 64% inhibition
Al3+
-
80% inhibition of xylan-inducible enzyme, 10% of xylose-inducible enzyme, at 1 mM
AlCl3
-
about 85% residual activity at 1 mM
alpha-L-arabinofuranose
competitive
arabinose
-
full activity is observed in the presence of up to 50 mM arabinose. Activity is reduced to 88%, 80% and 70% of maximum activity at 100 mM, 150 mM and 200 mM arabinose, respectively
benzyl 1-thio-beta-D-xylopyranoside
-
-
beta-D-xylopyranosyl azide
-
competitive, protects against inactivation by N-bromoacetyl-beta-D-xylopyranosylamine
beta-D-xylose
bis-(2-hydroxyethyl)amino-tris(hydroxymethyl)methane
-
-
Cd2+
Malbranchea pulchella var. sulfurea
-
50% inhibition at 1 mM
CdCl2
1 mM, 82% inhibition
CoCl2
-
10 mM, slight inhibition
CuCl2
1 mM, 83% inhibition
CuSO4
D-gluconate
-
1 mM, 87% inhibition
D-glucono-1,5-lactone
-
competitive
D-glucose
D-xylose
diethanolamine
-
-
diethyldicarbonate
-
inactivation indicates a carbethoxylation of histidine residues
dithiothreitol
-
49.2% residual activity at 50 mM
ethanolamine
-
-
FeSO4
-
about 65% residual activity at 1 mM
glucose
-
16% inhibition at 10 mM
guanidinium hydrochloride
-
50% inhibition at 3 M
HgCl2
iodoacetamide
-
highly inhibitory
iodoacetate
-
inhibition of beta-xylosidase A
K+
about 77% residual activity at 5 mM
L-arabinose
methyl-beta-D-xylopyranoside
-
34% inhibition at 10 mM
methylmethanethiolsulfonate
-
rapid decrease in activity, treatment with dithiothreitol restores activity to about 75%
MgSO4
-
10 mM, slight inhibition
MnSO4
-
10 mM, slight inhibition
N-Acetylimidazole
-
strong inhibition, 62% residual activity at 50 mM
N-bromoacetyl-beta-D-xylopyranosylamine
N-bromosuccinimide
n-butyl beta-D-xylopyranoside
-
pH above 7.5 affects the binding of the competitive inhibitor
N-ethyl-5-phenylisoxazolium-3'-sulfonate
-
i.e. Woodward’s reagent
NEM
80% inhibition at 1 mM
NiCl2
1 mM, 70% inhibition
o-nitrophenyl beta-D-xylopyranoside
-
substrate inhibition if concentration higher than 0.5 mM
p-chloro-mercuribenzoate
1 mM, 80% inhibition
p-chloromercuribenzoate
p-chloromercuryphenylsulfonic acid
potent inhibitor
p-hydroxymercuribenzoate
p-mercurithiosalicylate
-
80% inhibition at 1 mM
p-nitrophenol
-
noncompetitive
p-nitrophenyl alpha-L-arabinofuranoside
-
substrate inhibition if concentration higher than 12 mM
p-nitrophenyl alpha-L-arabinopyranoside
-
substrate inhibition if concentration higher than 9 mM
p-nitrophenyl beta-D-xylopyranoside
-
substrate inhibition if concentration higher than 0.1-0.2 mM
p-nitrophenyl beta-D-xyloside
-
inhibition at 6.2 mM
Sodium azide
-
10 mM, 5-6% inhibition
Sodium laurylsulfate
Malbranchea pulchella var. sulfurea
-
strong inhibition at 1 mM
triethanolamine
-
-
Tris(hydroxymethyl)aminomethane
-
-
Urea
-
100% inhibition at 3 M
xylobiose
xylose
ZnSO4
-
10 mM, 50% loss of activity
additional information
-