Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.37 extracted from

  • Rodionova, N.A.; Tavobilov, I.M.; Bezborodov, A.M.
    beta-xylosidase from Aspergillus niger 15: Purification and properties (1983), J. Appl. Biochem., 5, 300-312.
    View publication on PubMed
Search for more literature for 3.2.1.37

Inhibitors

Inhibitors Comment Organism Structure
beta-D-xylose competitive inhibition Aspergillus niger
p-chloromercuribenzoate
-
 Aspergillus niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.23
-
 p-nitrophenyl beta-D-xylopyranoside
-
 Aspergillus niger
0.67
-
 xylobiose
-
 Aspergillus niger

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus niger
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
122000
-
 2 * 122000, SDS-PAGE Aspergillus niger
253000
-
 gel filtration, SDS-PAGE Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylobiose + H2O Aspergillus niger transxylosylation activity beta-D-xylose + D-xylose
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity by 4step chromatography Aspergillus niger

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Aspergillus niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.22
-
-
 Aspergillus niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O low activity Aspergillus niger 2-hydroxymethylphenol + beta-D-glucose
-
 ?
2-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O sophorose, low activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
3-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O laminaribiose, low activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
4-nitrophenyl beta-D-glucopyranoside + H2O low activity Aspergillus niger 4-nitrophenol + D-glucopyranose
-
 ?
4-O-beta-D-glucopyranosyl-D-glucose + H2O cellobiose, 30% activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
6-O-beta-D-glucopyranosyl-D-glucose + H2O gentiobiose, low activity Aspergillus niger beta-D-glucose + D-glucose
-
 ?
methyl-beta-D-xyloside + H2O very low activity Aspergillus niger beta-D-xylose + methanol
-
 ?
p-nitrophenyl alpha-L-arabinoside + H2O low activity Aspergillus niger p-nitrophenol + alpha-L-arabinose
-
 ?
p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Aspergillus niger p-nitrophenol + beta-D-xylopyranose
-
 ?
phenyl 4-O-beta-D-glucopyranosyl-D-glucopyranoside + H2O 25% activity Aspergillus niger phenyl beta-D-glucopyranoside + D-glucose
-
 ?
phenyl beta-D-glucopyranoside + H2O low activity Aspergillus niger phenol + D-glucopyranose
-
 ?
xylobiose + H2O transxylosylation activity Aspergillus niger beta-D-xylose + D-xylose
-
 ?
xylopentaose + H2O
-
 Aspergillus niger beta-D-xylose + ?
-
 ?
xylotriose + H2O
-
 Aspergillus niger beta-D-xylose + xylobiose
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 122000, SDS-PAGE Aspergillus niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Aspergillus niger

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to 1 h Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.8 4
-
 Aspergillus niger

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 8 stable within Aspergillus niger