Literature summary for 3.2.1.37 extracted from

Katapodis, P.; Nerinckx, W.; Claeyssens, M.; Christakopoulos, P.
Purification and characterization of a thermostable intracellular beta-xylosidase from the thermophilic fungus Sporotrichum thermophile (2006), Proc. Biochem., 41, 2402-2409.

No PubMed abstract available

Search for more literature for 3.2.1.37

Inhibitors

Inhibitors	Comment	Organism	Structure
2,3-epoxypropyl beta-D-xylobioside	irreversible inactivation	Thermothelomyces heterothallicus
additional information	not inhibitory: alkyl epoxides derived from D-xylose	Thermothelomyces heterothallicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.1	-	p-nitrophenyl-beta-D-xylopyranoside	pH 7.0, 50°C	Thermothelomyces heterothallicus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
intracellular	-	Thermothelomyces heterothallicus	5622	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	x * 45000, SDS-PAGE	Thermothelomyces heterothallicus

Organic Solvent Stability

Organic Solvent	Comment	Organism
Ethanol	10% v/v, 30% decrease in activity	Thermothelomyces heterothallicus

Organism

Organism	UniProt	Comment	Textmining
Thermothelomyces heterothallicus	-	strain ATCC 34628	-

Purification (Commentary)

Purification (Comment)	Organism
-	Thermothelomyces heterothallicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
beta-D-xylose	-	Thermothelomyces heterothallicus	xylobiose + H2O	-	r
additional information	enzyme cleaves beta-1,4-linked xylo-oligosaccharides with chain length from 2 to 6, releasing xylose from the non-reducing end. When using high donor concentration, the enzyme catalyses the condensation reaction. No substrate: xylan	Thermothelomyces heterothallicus	?	-	?
p-nitrophenyl-beta-D-xylopyranoside + H2O	-	Thermothelomyces heterothallicus	p-nitrophenol + beta-D-xylopyranose	-	r
xylobiose + H2O	-	Thermothelomyces heterothallicus	2 beta-D-xylose	-	r
xylohexaose + H2O	80% of the activity with xylobiose	Thermothelomyces heterothallicus	beta-D-xylose	-	r
xylopentaose + H2O	83% of the activity with xylobiose	Thermothelomyces heterothallicus	beta-D-xylose	-	r
xylotetraose + H2O	86% of the activity with xylobiose	Thermothelomyces heterothallicus	xylotriose + beta-D-xylose	-	r
xylotriose + 2 H2O	92% of the activity with xylobiose	Thermothelomyces heterothallicus	3 D-xylose	-	r

Subunits

Subunits	Comment	Organism
?	x * 45000, SDS-PAGE	Thermothelomyces heterothallicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	-	Thermothelomyces heterothallicus

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
50	-	stable up to	Thermothelomyces heterothallicus
60	-	1 h, 58% residual activity	Thermothelomyces heterothallicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Thermothelomyces heterothallicus

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
6	8	-	Thermothelomyces heterothallicus

pI Value

Organism	Comment	pI Value Maximum	pI Value
Thermothelomyces heterothallicus	isoelectric focusing	-	4.2

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Release 2023.1 (January 2023)