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3.2.1.37: xylan 1,4-beta-xylosidase

This is an abbreviated version!
For detailed information about xylan 1,4-beta-xylosidase, go to the full flat file.

Word Map on EC 3.2.1.37

Reaction

(Xylbeta(1-4))x
+
H2O
=
beta-D-xylopyranose
 +
(Xylbeta(1-4))x-1

Synonyms

1,4-beta-D-xylan xylohydrolase, 1,4-beta-D-xylosidase, 1.4-beta-D-xylanase, exo-type, ABF3, AbfA, AbfB, AbXyl, alpha-arabinofuranosidase/beta-xylosidase, alpha-L-arabinofuranosidase, alpha-L-arabinofuranosidase 3, alpha-L-arabinofuranoside arabinofuranohydrolase, arabinofuranosidase 3, AXH-d3, Axy43A, BcXyl, beta-1,4-xylosidase, beta-D-xylopyranosidase, beta-D-xylosidase, beta-D-xylosidase/alpha-L-arabinofuranosidase, beta-D-xyloside xylohydrolase, beta-glucosidase/xylosidase, beta-xylosidase, beta-xylosidase A, beta-xylosidase B, beta-xylosidase I, beta-xylosidase II, beta-xylosidase-1, beta-xylosidase-2, beta-xylosidase-3, beta-xylosidase-4, betaXTE, betaXTR, bifunctional beta-glucosidase/xylosidase, BlxA, Bxl1, BxTW1, Bxyl, ccxyl3a, EcXyl43 beta-xylosidase, exo-1,4-beta-D-xylosidase, exo-1,4-beta-xylosidase, Exo-beta-(1,4)-xylanase, GH43 beta-1,4-xylosidase, GH43 xylosidase, GH43B6, glycoside hydrolase family 3 beta-xylosidase, More, Pae1263, RrXyl43A, Rubg3A, Rubg3B, RuBGX1, RuXyn1, SSO3032, Sxa, Thxyl43A, Tpexyl3, X12345, Xln-DT, Xyl, Xyl I, XYL1, Xyl3A, XYL4, xyl43, xyl43A, Xyl43C, Xyl5, XYLA, Xylan 1,4-beta-xylosidase, xylanase, XylB, XylC, XylCD2, xylobiase, xylosidase, XynB, XynB2, XynB3

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.37 xylan 1,4-beta-xylosidase

Engineering

Engineering on EC 3.2.1.37 - xylan 1,4-beta-xylosidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D128G
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme, nearly inactive mutant
D15G
site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme, nearly inactive mutant
E160A
-
E160 is the acid-base catalyst
E178G
site-directed mutagenesis, the mutant shows an altered substrate specificity and highly reduced activity compared to the wild-type enzyme
E335G
nucleophile-deficient mutant enzyme can function as an efficient glycosynthase, using alpha-D-xylopyranosyl fluoride as a donor and various aryl sugars as acceptors. The mutant enzyme can also catalyze the self-condensation reaction of alpha-D-xylopyranosyl fluoride, providing mainly alpha-D-xylobiosyl fluoride
E160A
-
E160 is the acid-base catalyst
-
E335G
-
nucleophile-deficient mutant enzyme can function as an efficient glycosynthase, using alpha-D-xylopyranosyl fluoride as a donor and various aryl sugars as acceptors. The mutant enzyme can also catalyze the self-condensation reaction of alpha-D-xylopyranosyl fluoride, providing mainly alpha-D-xylobiosyl fluoride
-
biofuel production
D269A
no catalytic activity
D269N
no catalytic activity
E594Q
activity similar to wild-type
E616A
about 5% of wild-type activity
R149L
no catalytic activity
Y237F
less than 10% of wild-type activity
E186A
-
The E186A mutation abolishes the pKa assigned to the catalytic acid E186, mutant enzyme binds only the neutral aminoalcohol triethanolamine, whereas wild-type enzyme binds only the cationic aminoalcohol. At pH 7.0 and 25°C, the ratio of kcat values for substrates 4-nitrophenyl-beta-D-xylopyranoside and 4-nitrophenyl-alpha-L-arabinofuranoside of E186A is 100fold that of wild-type enzyme
E160A
-
E160 is the acid-base catalyst
additional information