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Literature summary for 3.2.1.37 extracted from
- Isolation and properties of extracellular beta-xylosidases from fungi Aspergillus japonicus and Trichoderma reesei (2009), Biochemistry, 74, 1002-1008.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-nitrophenyl-beta-D-xylopyranoside | - |
Aspergillus japonicus |  |
| 4-nitrophenyl-beta-D-xylopyranoside | - |
Trichoderma reesei | |
| xylose | competitive | Aspergillus japonicus | |
| xylose | competitive | Trichoderma reesei | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.33 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Aspergillus japonicus | |
| 4.4 | - |
Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl | pH 5.0, 40°C | Aspergillus japonicus | |
| 4.6 | - |
Xyl-beta-(1->4)-Xyl | pH 5.0, 40°C | Aspergillus japonicus | |
| 5.7 | - |
Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl | pH 5.0, 40°C | Aspergillus japonicus | |
| 7.2 | - |
xylobiose | pH 5.0, 40°C | Trichoderma reesei | |
| 7.2 | - |
xylotriose | pH 5.0, 40°C | Trichoderma reesei | |
| 7.2 | - |
xylotetraose | pH 5.0, 40°C | Trichoderma reesei | |
| 12 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Trichoderma reesei | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus japonicus | - |
- |
- |
| Trichoderma reesei | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.1 | - |
substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C | Trichoderma reesei |
| 0.1 | - |
substrate arabinoxylan, pH 5.0, 40!C | Aspergillus japonicus |
| 0.9 | - |
substrate arabinoxylan, pH 5.0, 40°C | Trichoderma reesei |
| 3.9 | - |
substrate 4-nitrophenyl-alpha-L-arabinofuranoside, pH 5.0, 40°C | Aspergillus japonicus |
| 7 | - |
substrate glucuronoxylan, pH 5.0, 40°C | Aspergillus japonicus |
| 14.2 | - |
substrate glucuronoxylan, pH 5.0, 40°C | Trichoderma reesei |
| 16 | - |
substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 5.0, 40°C | Trichoderma reesei |
| 25 | - |
substrate xylobiose, pH 5.0, 40°C | Trichoderma reesei |
| 28.4 | - |
substrate Xyl-beta-(1->4)-Xyl, pH 5.0, 40°C | Aspergillus japonicus |
| 42.6 | - |
substrate 4-nitrophenyl-beta-D-xylopyranoside, pH 5.0, 40°C | Aspergillus japonicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Aspergillus japonicus | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Trichoderma reesei | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Aspergillus japonicus | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| arabinoxylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
| arabinoxylan + H2O | - |
Aspergillus japonicus | ? | - |
? | |
| glucuronoxylan + H2O | - |
Trichoderma reesei | ? | - |
? | |
| glucuronoxylan + H2O | - |
Aspergillus japonicus | ? | - |
? | |
| additional information | no substrate: xyloglucan | Trichoderma reesei | ? | - |
? | |
| additional information | no substrate: xyloglucan | Aspergillus japonicus | ? | - |
? | |
| additional information | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Trichoderma reesei | ? | - |
? | |
| additional information | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Aspergillus japonicus | ? | - |
? | |
| Xyl-beta-(1->4)-Xyl + H2O | - |
Aspergillus japonicus | 2 D-xylose | - |
? | |
| Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl + H2O | - |
Aspergillus japonicus | 3 D-xylose | - |
? | |
| Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl-beta-(1->4)-Xyl + H2O | - |
Aspergillus japonicus | 4 D-xylose | - |
? | |
| xylobiose + H2O | - |
Trichoderma reesei | 2 D-xylose | - |
? | |
| xylotetraose + 3 H2O | - |
Trichoderma reesei | 4 D-xylose | - |
? | |
| xylotriose + H2O | - |
Trichoderma reesei | 3 D-xylose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Trichoderma reesei |
| 70 | - |
- |
Aspergillus japonicus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
3 h, no loss of activity | Trichoderma reesei |
| 60 | - |
3 h, no loss of activity | Aspergillus japonicus |
| 60 | - |
3 h, 52% residual activity | Trichoderma reesei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
- |
Trichoderma reesei |
| 4 | - |
- |
Aspergillus japonicus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.4 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Trichoderma reesei | |
| 2.9 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.0, 40°C | Aspergillus japonicus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Trichoderma reesei |
| physiological function | the beta-xylosidase exhibits synergism with endoxylanase upon hydrolysis of glucuronoxylan as well as with alpha-L-arabinofuranosidase and endoxylanase upon hydrolysis of arabinoxylan. Addition of beta-xylosidases increases efficiency of hydrolysis of plant raw materials with high hemicellulose content such as maize cobs by the enzymic preparation Celloviridine G20x depleted of its own beta-xylosidase | Aspergillus japonicus |
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