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Literature summary for 3.2.1.37 extracted from

  • Li, Y.K.; Yao, H.J.; Cho, Y.T.
    Effective induction, purification and characterization of Trichoderma koningii G-39 beta-xylosidase with high transferase activity (2000), Biotechnol. Appl. Biochem., 31, 119-125.
No PubMed abstract available
Search for more literature for 3.2.1.37

Application

Application Comment Organism
synthesis synthesis of a group of uncommon xylosides via the transxylosylation activity Trichoderma koningii

Inhibitors

Inhibitors Comment Organism Structure
beta-D-xylose moderate product inhibition, Ki: 5 mM Trichoderma koningii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2,4-dinitrophenyl beta-D-xylopyranoside
-
 Trichoderma koningii
0.02
-
 4-chloro-2-nitrophenyl beta-D-xylopyranoside
-
 Trichoderma koningii
0.03
-
 o-nitrophenyl beta-D-xylopyranoside
-
 Trichoderma koningii
0.04
-
 p-nitrophenyl beta-D-xylopyranoside
-
 Trichoderma koningii
7.5
-
 p-nitrophenyl-alpha-L-arabinopyranoside
-
 Trichoderma koningii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Trichoderma koningii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000 105000 gel filtration, SDS-PAGE Trichoderma koningii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylo-oligosaccharides + H2O Trichoderma koningii derived from xylan beta-D-xylose + ?
-
 ?

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile stable in the presence of 20%, v/v Trichoderma koningii
Methanol stable in the presence of alcohols, 20%, v/v Trichoderma koningii

Organism

Organism UniProt Comment Textmining
Trichoderma koningii
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Trichoderma koningii

Purification (Commentary)

Purification (Comment) Organism
near homogeneity, anion exchange chromatography Trichoderma koningii

Source Tissue

Source Tissue Comment Organism Textmining
mycelium induced enzyme production by addition of 1% xylan and 0.1% xylose in the medium Trichoderma koningii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.02
-
-
 Trichoderma koningii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,4-dinitrophenyl beta-D-xylopyranoside + H2O broad tolerance for the aglycone portion of the substrate Trichoderma koningii 2,4-dinitrophenol + beta-D-xylopyranose
-
 ?
4-chloro-2-nitrophenyl beta-D-xylopyranoside + H2O
-
 Trichoderma koningii 4-chloro-2-nitrophenol + beta-D-xylopyranose
-
 ?
4-nitrophenyl beta-D-xylopyranoside + methanol transxylosylation activity, NMR study in the presence of 15% methanol, v/v Trichoderma koningii 4-nitrophenol + methyl beta-D-xylopyranoside very weak peaks of D-xylose-alpha-anomers, most D-xylose-beta-anomers ?
o-nitrophenyl beta-D-xylopyranoside + H2O
-
 Trichoderma koningii o-nitrophenol + beta-D-xylopyranose
-
 ?
p-nitrophenyl alpha-L-arabinopyranoside + H2O
-
 Trichoderma koningii p-nitrophenol + alpha-L-arabinopyranose
-
 ?
p-nitrophenyl beta-D-xylopyranoside + H2O
-
 Trichoderma koningii p-nitrophenol + beta-D-xylopyranose
-
 ?
xylo-oligosaccharides + H2O derived from xylan Trichoderma koningii beta-D-xylose + ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000-105000, gel filtration, SDS-PAGE Trichoderma koningii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Trichoderma koningii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 quite stable below Trichoderma koningii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5 4
-
 Trichoderma koningii

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 7.4 at 37°C, very unstable below pH 1.7 and above pH 9.5 Trichoderma koningii