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Literature summary for 3.2.1.37 extracted from
- Characterization of a Paenibacillus woosongensis beta-xylosidase/alpha-arabinofuranosidase produced by recombinant Escherichia coli (2010), J. Microbiol. Biotechnol., 20, 1711-1716.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Paenibacillus woosongensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 5 mM, 79% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 68% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis |  |
| Fe2+ | 5 mM, 57% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 12% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis | |
| Fe3+ | 5 mM, 170% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 49% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis | |
| SDS | 5 mM, 70% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 112% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe3+ | 5 mM, 170% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 49% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis | |
| SDS | 5 mM, 70% residual activity with substrate 4-nitrophenyl beta-D-xylopyranoside, 112% activity with substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus woosongensis | E7DXB6 | bifunctional beta-xylosidase/alpha-arabinofuranosidase | - |
| Paenibacillus woosongensis DSM 16971 | E7DXB6 | bifunctional beta-xylosidase/alpha-arabinofuranosidase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinasnt protein | Paenibacillus woosongensis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 347.3 | - |
substrate 4-nitrophenyl alpha-L-arabinofuranoside, pH 7.0, 45°C | Paenibacillus woosongensis |
| 659.9 | - |
substrate 4-nitrophenyl beta-D-xylopyranoside, pH 6.5, 50°C | Paenibacillus woosongensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Paenibacillus woosongensis | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Paenibacillus woosongensis DSM 16971 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl alpha-L-arabinofuranoside + H2O | - |
Paenibacillus woosongensis KCTC 3953 (DSM 16971) | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Paenibacillus woosongensis | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Paenibacillus woosongensis DSM 16971 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
| 4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Paenibacillus woosongensis KCTC 3953 (DSM 16971) | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53000-54000, SDS-PAGE | Paenibacillus woosongensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XylC | - |
Paenibacillus woosongensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis |
| 50 | - |
substrate 4-nitrophenyl beta-D-xylopyranoside | Paenibacillus woosongensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
3 h, stable | Paenibacillus woosongensis |
| 40 | - |
3 h, 83% residual activity | Paenibacillus woosongensis |
| 45 | - |
rapid decrease in activity above | Paenibacillus woosongensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
substrate 4-nitrophenyl beta-D-xylopyranoside | Paenibacillus woosongensis |
| 7 | - |
substrate 4-nitrophenyl alpha-L-arabinofuranoside | Paenibacillus woosongensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7 | more than 80% of maximum activity within | Paenibacillus woosongensis |
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