Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | beta-D-xylosidase activity is strongly stimulated and protected against heat inactivation by calcium ions | Mycothermus thermophilus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, 100% inhibition | Mycothermus thermophilus | |
EDTA | 1 mM, 20% inhibition | Mycothermus thermophilus | |
Hg2+ | 1 mM, 91% inhibition | Mycothermus thermophilus | |
additional information | not inhibitory: D-xylose up to 200 mM, Mg2+, Al3+, Co2+, Mn2+, Cu2+, K+, Ba2+, Fe2+ or Zn2+ at 1.0 mM, 2-mercaptoethanol | Mycothermus thermophilus | |
Zn2+ | 1 mM, 22% inhibition | Mycothermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
4-nitrophenyl beta-D-glucopyranoside | pH 5.0, 60°C | Mycothermus thermophilus | |
1.3 | - |
4-nitrophenyl beta-D-xylopyranoside | pH 5.0, 60°C | Mycothermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
gel filtration | Mycothermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycothermus thermophilus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | carbohydrate content 12% | Mycothermus thermophilus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Mycothermus thermophilus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
65 | - |
substrate 4-nitrophenyl beta-D-xylopyranoside, pH 5.0, 60°C | Mycothermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-glucopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | - |
Mycothermus thermophilus | 4-nitrophenol + D-xylose | - |
? | |
xylobiose + H2O | - |
Mycothermus thermophilus | 2 D-xylose | - |
? | |
xylotetraose + 3 H2O | - |
Mycothermus thermophilus | 4 D-xylose | - |
? | |
xylotriose + 2 H2O | - |
Mycothermus thermophilus | 3 D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Mycothermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Mycothermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 h, absence of substrate, stable | Mycothermus thermophilus |
65 | - |
half-lives are 11 and 30 min in the absence or presence of calcium, respectively | Mycothermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Mycothermus thermophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6 | - |
Mycothermus thermophilus |
Organism | Comment | Expression |
---|---|---|
Mycothermus thermophilus | expression is induced by growth on xylan | up |