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Literature summary for 3.2.1.37 extracted from

  • Wagschal, K.; Heng, C.; Lee, C.; Robertson, G.; Orts, W.; Wong, D.
    Purification and characterization of a glycoside hydrolase family 43 beta-xylosidase from Geobacillus thermoleovorans IT-08 (2009), Appl. Biochem. Biotechnol., 155, 304-313.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Geobacillus thermoleovorans

Inhibitors

Inhibitors Comment Organism Structure
4-methylumbelliferyl-alpha-L-arabinofuranoside substrate inhibition Geobacillus thermoleovorans
4-methylumbelliferyl-beta-D-xylopyranoside substrate inhibition Geobacillus thermoleovorans
D-xylose competitive Geobacillus thermoleovorans
L-arabinose competitive Geobacillus thermoleovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.081
-
4-methylumbelliferyl-alpha-L-arabinofuranoside pH 6.2, 40°C Geobacillus thermoleovorans
0.366
-
4-methylumbelliferyl-beta-D-xylopyranoside pH 6.2, 40°C Geobacillus thermoleovorans
0.497
-
4-nitrophenyl-alpha-L-arabinofuranoside pH 6.2, 40°C Geobacillus thermoleovorans
0.55
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.2, 40°C Geobacillus thermoleovorans
5.09
-
xylotriose pH 6.2, 40°C Geobacillus thermoleovorans
12.8
-
xylobiose pH 6.2, 40°C Geobacillus thermoleovorans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58100
-
x * 58100, calculated Geobacillus thermoleovorans

Organism

Organism UniProt Comment Textmining
Geobacillus thermoleovorans Q2I2N4
-
-
Geobacillus thermoleovorans IT-08 Q2I2N4
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
Geobacillus thermoleovorans 4-methylumbelliferol + alpha-L-arabinofuranose
-
?
4-methylumbelliferyl-alpha-L-arabinofuranoside + H2O
-
Geobacillus thermoleovorans IT-08 4-methylumbelliferol + alpha-L-arabinofuranose
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O substrate inhibition Geobacillus thermoleovorans 4-methylumbelliferol + beta-D-xylose
-
?
4-methylumbelliferyl-beta-D-xylopyranoside + H2O substrate inhibition Geobacillus thermoleovorans IT-08 4-methylumbelliferol + beta-D-xylose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside Geobacillus thermoleovorans 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside Geobacillus thermoleovorans IT-08 4-nitrophenol + alpha-L-arabinofuranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside Geobacillus thermoleovorans 4-nitrophenol + beta-D-xylopyranose
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside Geobacillus thermoleovorans IT-08 4-nitrophenol + beta-D-xylopyranose
-
?
beechwood arabinoxylan + H2O
-
Geobacillus thermoleovorans D-xylose + ?
-
?
birch arabinoxylan + H2O
-
Geobacillus thermoleovorans D-xylose + ?
-
?
xylobiose + H2O
-
Geobacillus thermoleovorans 2 D-xylose
-
?
xylobiose + H2O
-
Geobacillus thermoleovorans IT-08 2 D-xylose
-
?
xylopentaose + H2O
-
Geobacillus thermoleovorans 5 D-xylose
-
?
xylotriose + H2O
-
Geobacillus thermoleovorans 3 D-xylose
-
?

Subunits

Subunits Comment Organism
? x * 58100, calculated Geobacillus thermoleovorans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
54
-
stable up to Geobacillus thermoleovorans
57.5
-
half-life 35 min Geobacillus thermoleovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
xylotriose pH 6.2, 40°C Geobacillus thermoleovorans
0.065
-
xylobiose pH 6.2, 40°C Geobacillus thermoleovorans
0.18
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.2, 40°C Geobacillus thermoleovorans
0.208
-
4-methylumbelliferyl-beta-D-xylopyranoside pH 6.2, 40°C Geobacillus thermoleovorans
0.233
-
4-methylumbelliferyl-alpha-L-arabinofuranoside pH 6.2, 40°C Geobacillus thermoleovorans
1.18
-
4-nitrophenyl-alpha-L-arabinofuranoside pH 6.2, 40°C Geobacillus thermoleovorans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Geobacillus thermoleovorans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.8
-
L-arabinose pH 6.2, 40°C Geobacillus thermoleovorans
76
-
D-xylose pH 6.2, 40°C Geobacillus thermoleovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0039
-
xylotriose pH 6.2, 40°C Geobacillus thermoleovorans
0.0051
-
xylobiose pH 6.2, 40°C Geobacillus thermoleovorans
0.33
-
4-nitrophenyl-beta-D-xylopyranoside pH 6.2, 40°C Geobacillus thermoleovorans
0.57
-
4-methylumbelliferyl-beta-D-xylopyranoside pH 6.2, 40°C Geobacillus thermoleovorans
2.37
-
4-nitrophenyl-alpha-L-arabinofuranoside pH 6.2, 40°C Geobacillus thermoleovorans
2.88
-
4-methylumbelliferyl-alpha-L-arabinofuranoside pH 6.2, 40°C Geobacillus thermoleovorans