Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Geobacillus thermoleovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-methylumbelliferyl-alpha-L-arabinofuranoside | substrate inhibition | Geobacillus thermoleovorans | |
4-methylumbelliferyl-beta-D-xylopyranoside | substrate inhibition | Geobacillus thermoleovorans | |
D-xylose | competitive | Geobacillus thermoleovorans | |
L-arabinose | competitive | Geobacillus thermoleovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.081 | - |
4-methylumbelliferyl-alpha-L-arabinofuranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.366 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.497 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.55 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
5.09 | - |
xylotriose | pH 6.2, 40°C | Geobacillus thermoleovorans | |
12.8 | - |
xylobiose | pH 6.2, 40°C | Geobacillus thermoleovorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58100 | - |
x * 58100, calculated | Geobacillus thermoleovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus thermoleovorans | Q2I2N4 | - |
- |
Geobacillus thermoleovorans IT-08 | Q2I2N4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus thermoleovorans | 4-methylumbelliferol + alpha-L-arabinofuranose | - |
? | |
4-methylumbelliferyl-alpha-L-arabinofuranoside + H2O | - |
Geobacillus thermoleovorans IT-08 | 4-methylumbelliferol + alpha-L-arabinofuranose | - |
? | |
4-methylumbelliferyl-beta-D-xylopyranoside + H2O | substrate inhibition | Geobacillus thermoleovorans | 4-methylumbelliferol + beta-D-xylose | - |
? | |
4-methylumbelliferyl-beta-D-xylopyranoside + H2O | substrate inhibition | Geobacillus thermoleovorans IT-08 | 4-methylumbelliferol + beta-D-xylose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside | Geobacillus thermoleovorans | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl alpha-L-arabinofuranoside + H2O | greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside | Geobacillus thermoleovorans IT-08 | 4-nitrophenol + alpha-L-arabinofuranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside | Geobacillus thermoleovorans | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | greater catalytic efficiency for hydrolysis of 4-nitrophenyl-alpha-L-arabinofuranoside than of 4-nitrophenyl-beta-D-xylopyranoside | Geobacillus thermoleovorans IT-08 | 4-nitrophenol + beta-D-xylopyranose | - |
? | |
beechwood arabinoxylan + H2O | - |
Geobacillus thermoleovorans | D-xylose + ? | - |
? | |
birch arabinoxylan + H2O | - |
Geobacillus thermoleovorans | D-xylose + ? | - |
? | |
xylobiose + H2O | - |
Geobacillus thermoleovorans | 2 D-xylose | - |
? | |
xylobiose + H2O | - |
Geobacillus thermoleovorans IT-08 | 2 D-xylose | - |
? | |
xylopentaose + H2O | - |
Geobacillus thermoleovorans | 5 D-xylose | - |
? | |
xylotriose + H2O | - |
Geobacillus thermoleovorans | 3 D-xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58100, calculated | Geobacillus thermoleovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
54 | - |
stable up to | Geobacillus thermoleovorans |
57.5 | - |
half-life 35 min | Geobacillus thermoleovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
xylotriose | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.065 | - |
xylobiose | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.18 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.208 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.233 | - |
4-methylumbelliferyl-alpha-L-arabinofuranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
1.18 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 6.2, 40°C | Geobacillus thermoleovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Geobacillus thermoleovorans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
6.8 | - |
L-arabinose | pH 6.2, 40°C | Geobacillus thermoleovorans | |
76 | - |
D-xylose | pH 6.2, 40°C | Geobacillus thermoleovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0039 | - |
xylotriose | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.0051 | - |
xylobiose | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.33 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
0.57 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
2.37 | - |
4-nitrophenyl-alpha-L-arabinofuranoside | pH 6.2, 40°C | Geobacillus thermoleovorans | |
2.88 | - |
4-methylumbelliferyl-alpha-L-arabinofuranoside | pH 6.2, 40°C | Geobacillus thermoleovorans |