Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1.7fold activation of xylan-inducible enzyme, 1.1fold of xylose-inducible enzyme, at 0.25 mM | Aspergillus versicolor | |
Ca2+ | 2.2fold activation of xylan-inducible enzyme, 1.4fold of xylose-inducible enzyme, at 0.25 mM | Aspergillus versicolor | |
Co2+ | 1.4fold activation of xylan-inducible enzyme, 1.2fold of xylose-inducible enzyme, at 1 mM | Aspergillus versicolor | |
Mg2+ | 1.65fold activation of xylan-inducible enzyme, 1.2fold of xylose-inducible enzyme, at 0.25 mM | Aspergillus versicolor | |
Mn2+ | 1.2fold activation of xylan-inducible enzyme, 1.2fold of xylose-inducible enzyme, at 1 mM | Aspergillus versicolor | |
additional information | xylose and xylan as only carbon source induce expression of enzyme form 1 and enzyme form 2, respectively, prevented by cycloheximide | Aspergillus versicolor | |
Zn2+ | 1.2fold activation of xylan-inducible enzyme, 1.3fold of xylose-inducible enzyme, at 1 mM | Aspergillus versicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-nitrophenyl-beta-D-xylopyranoside | - |
Aspergillus versicolor | |
Al3+ | 80% inhibition of xylan-inducible enzyme, 10% of xylose-inducible enzyme, at 1 mM | Aspergillus versicolor | |
Cu2+ | 64% inhibition of xylan-inducible enzyme, 79% of xylose-inducible enzyme, at 1 mM | Aspergillus versicolor | |
D-xylose | product inhibition | Aspergillus versicolor | |
Hg2+ | complete inhibition of xylan-inducible enzyme and xylose-inducible enzyme, at 1 mM | Aspergillus versicolor | |
additional information | addition of glucose to the growth medium suppresses enzyme expression | Aspergillus versicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 45°C, xylan-inducible enzyme | Aspergillus versicolor | |
0.32 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.6, 40°C, xylose-inducible enzyme | Aspergillus versicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no effect by 2-mercaptoethanol | Aspergillus versicolor |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
xylose-inducible enzyme, gel filtration | Aspergillus versicolor |
100000 | - |
xylan-inducible enzyme, gel filtration | Aspergillus versicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus versicolor | - |
two enzyme forms | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | O-linked sugars | Aspergillus versicolor |
Purification (Comment) | Organism |
---|---|
two forms of native enzyme from mycelium by ion exchange chromatography, xylose-inducible enzyme form 11.3fold, xylan.inducible enzyme form 12.3fold, ammonium sulfate, and gel filtration | Aspergillus versicolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | xylose and xylan as only carbon source induce expression of enzyme form 1 and enzyme form 2, respectively, addition of glucose suppresses enzyme expression | Aspergillus versicolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the xylan-inducible enzyme shows higher catalytic efficiency than the xylose-inducible enzyme | Aspergillus versicolor |
51.3 | - |
purified xylan-inducible enzyme | Aspergillus versicolor |
59.6 | - |
purified xylose-inducible enzyme | Aspergillus versicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactopyranoside + H2O | xylan-inducible enzyme shows 10% of activity with 4-nitrophenyl-beta-D-xylopyranoside, and xylose-inducible enzyme 2% | Aspergillus versicolor | 4-nitrophenol + beta-D-galactose | - |
? | |
4-nitrophenyl beta-D-glucopyranoside + H2O | xylan-inducible enzyme shows 18% of activity with 4-nitrophenyl-beta-D-xylopyranoside, and xylose-inducible enzyme 8% | Aspergillus versicolor | 4-nitrophenol + beta-D-glucose | - |
? | |
4-nitrophenyl beta-D-xylopyranoside + H2O | preferred substrate, specific for, xylan-inducible enzyme and xylose-inducible enzyme | Aspergillus versicolor | 4-nitrophenol + beta-D-xylose | - |
? | |
4-nitrophenyl-alpha-L-arabinopyranoside + H2O | xylan-inducible enzyme shows 18% of activity with 4-nitrophenyl-beta-D-xylopyranoside, and xylose-inducible enzyme 26% | Aspergillus versicolor | 4-nitrophenol + alpha-L-arabinose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta-xylosidase | - |
Aspergillus versicolor |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
xylose-inducible enzyme | Aspergillus versicolor |
45 | - |
xylan-inducible enzyme | Aspergillus versicolor |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
half-life of the xylan-inducible enzyme is 9 min, of the xylose-inducible enzyme 18 min | Aspergillus versicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
xylan-inducible enzyme | Aspergillus versicolor |
6 | - |
xylose-inducible enzyme | Aspergillus versicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 6.5, 45°C, xylan-inducible enzyme | Aspergillus versicolor | |
5.3 | - |
4-nitrophenyl-beta-D-xylopyranoside | pH 5.6, 40°C, xylose-inducible enzyme | Aspergillus versicolor |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Aspergillus versicolor | xylose-inducible enzyme, isoelectric focusing | - |
5.6 |
Aspergillus versicolor | xylan-inducible enzyme, isoelectric focusing | - |
6.5 |