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Literature summary for 3.2.1.37 extracted from
- Purification and characterization of beta-xylosidase activities from yeast Arxula adeninivorans (1992), J. Basic Microbiol., 32, 159-166.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| beta-D-xylose | competitive inhibition | Blastobotrys adeninivorans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.23 | - |
p-nitrophenyl beta-D-xylopyranoside | for beta-xylosidase 1 | Blastobotrys adeninivorans | |
| 0.26 | - |
p-nitrophenyl beta-D-xylopyranoside | for beta-xylosidase 2 | Blastobotrys adeninivorans | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell wall | - |
Blastobotrys adeninivorans | 5618 | - |
| extracellular | - |
Blastobotrys adeninivorans | - |
- |
| intracellular | - |
Blastobotrys adeninivorans | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | - |
extra- and intracellular activity of beta-xylosidase-1, gel filtration, SDS-PAGE | Blastobotrys adeninivorans |
| 160000 | - |
beta-xylosidase-2, gel filtration, SDS-PAGE | Blastobotrys adeninivorans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylo-oligosaccharides + H2O | Blastobotrys adeninivorans | - |
beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | Blastobotrys adeninivorans | - |
beta-D-xylose + D-xylose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Blastobotrys adeninivorans | - |
yeast | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | active enzyme after deglycosylation by endoglycosidase H | Blastobotrys adeninivorans |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity by chromatography steps, separation of beta-xylosidase 1 and beta-xylosidase 2 | Blastobotrys adeninivorans |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | synthesis of enzyme only in xylose or methyl-beta-D-xylopyranoside media | Blastobotrys adeninivorans | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 21.1 | - |
extracellular beta-xylosidase 2 | Blastobotrys adeninivorans |
| 21.8 | - |
intracellular beta-xylosidase 2 | Blastobotrys adeninivorans |
| 23.7 | - |
cell-wall bound enzyme | Blastobotrys adeninivorans |
| 26.6 | - |
intracellular beta-xylosidase 1 | Blastobotrys adeninivorans |
| 27.1 | - |
extracellular beta-xylosidase 1 | Blastobotrys adeninivorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| methyl-beta-D-xyloside + H2O | - |
Blastobotrys adeninivorans | beta-D-xylose + methanol | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | high activity | Blastobotrys adeninivorans | p-nitrophenol + beta-D-xylopyranose | - |
? | |
| xylo-oligosaccharides + H2O | - |
Blastobotrys adeninivorans | beta-D-xylose + ? | - |
? | |
| xylobiose + H2O | - |
Blastobotrys adeninivorans | beta-D-xylose + D-xylose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 60000-75000, beta-xylosidase-2, SDS-PAGE | Blastobotrys adeninivorans |
| monomer | 1 * 60000-75000, beta-xylosidase-1, SDS-PAGE, gel filtration | Blastobotrys adeninivorans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-xylosidase-1 | - |
Blastobotrys adeninivorans |
| beta-xylosidase-2 | - |
Blastobotrys adeninivorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 64 | - |
Blastobotrys adeninivorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 5.2 | - |
Blastobotrys adeninivorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.1 | 6.4 | 50% activity at pH 4.1 and 6.4 | Blastobotrys adeninivorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
more stable than at pH 4.1 | Blastobotrys adeninivorans |
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