3.2.1.31: beta-glucuronidase
This is an abbreviated version!
For detailed information about beta-glucuronidase, go to the full flat file.
Word Map on EC 3.2.1.31
-
3.2.1.31
-
lysosomal
-
urine
-
urinary
-
neutrophil
-
bile
-
leukocyte
-
granule
-
lysozyme
-
polymorphonuclear
-
hydrolases
-
protoplasts
-
mucopolysaccharidosis
-
agrobacterium
-
cathepsins
-
cytochemical
-
biliary
-
tumefaciens
-
cauliflower
-
n-acetyl-beta-d-glucosaminidase
-
beta-n-acetylglucosaminidase
-
alpha-mannosidase
-
degranulation
-
glycosaminoglycans
-
sulfatase
-
glucuronic
-
azurophilic
-
beta-hexosaminidase
-
nitroreductase
-
nptii
-
hygromycin
-
zymosan
-
alpha-galactosidase
-
bombard
-
calli
-
glycosidases
-
agrobacterium-mediated
-
deconjugation
-
neomycin
-
unconjugated
-
beta-d-galactosidase
-
arylsulphatase
-
fmlp
-
diagnostics
-
alpha-l-fucosidase
-
synthesis
-
coliforms
-
medicine
-
biotechnology
-
alpha-naphthyl
-
toxicology
-
pomatia
-
analysis
-
hexosaminidase
-
crevicular
-
1,2-dimethylhydrazine
-
drug development
-
nutrition
-
molecular biology
-
alpha-l-iduronidase
- 3.2.1.31
- lysosomal
- urine
- urinary
- neutrophil
- bile
- leukocyte
- granule
- lysozyme
-
polymorphonuclear
- hydrolases
-
protoplasts
- mucopolysaccharidosis
- agrobacterium
- cathepsins
-
cytochemical
- biliary
- tumefaciens
- cauliflower
-
n-acetyl-beta-d-glucosaminidase
- beta-n-acetylglucosaminidase
- alpha-mannosidase
-
degranulation
- glycosaminoglycans
-
sulfatase
-
glucuronic
-
azurophilic
- beta-hexosaminidase
- nitroreductase
- nptii
- hygromycin
- zymosan
- alpha-galactosidase
-
bombard
-
calli
- glycosidases
-
agrobacterium-mediated
-
deconjugation
- neomycin
-
unconjugated
- beta-d-galactosidase
- arylsulphatase
- fmlp
- diagnostics
- alpha-l-fucosidase
- synthesis
-
coliforms
- medicine
- biotechnology
-
alpha-naphthyl
- toxicology
- pomatia
- analysis
- hexosaminidase
-
crevicular
- 1,2-dimethylhydrazine
- drug development
- nutrition
- molecular biology
- alpha-l-iduronidase
Reaction
Synonyms
beta-D-glucuronidase, Beta-D-glucuronoside glucuronosohydrolase, Beta-G1, beta-Gluc, beta-glucuronidase, beta-glucuronidase A, betaG, BLG, exo-beta-D-glucuronidase, exo-beta-glucuronidase, GlcAase, glucuronidase, beta-glucuronide glucuronohydrolase, GUR, GUS, Gus2, GusA, GusB, hGUSB, ketodase, More, PGUS, TM1062, TmGUSI, UidA
ECTree
3.2.1.31 beta-glucuronidaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 3.2.1.31 - beta-glucuronidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(1S)-1-(chloromethyl)-3-([5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]carbonyl)-2,3-dihydro-1H-benzo[e]indol-5-yl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + [(1S)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indol-3-yl][5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]methanone
-
-
-
-
?
(1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-{[({2-[({[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy}carbonyl)amino]-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl}oxy)carbonyl]amino}-alpha-D-lyxo-hexopyranoside + H2O
D-glucuronate + (1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-[({[2-({[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl}amino)-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl]oxy}carbonyl)amino]-alpha-D-lyxo-hexopyranoside
-
-
-
-
?
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2 + H2O
D-glucuronate + ?
-
no activity with enzyme acting on chondroitin, 142% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3 + H2O
D-glucuronate + ?
-
no activity with enzyme acting on chondroitin, 63% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2 + H2O
D-glucuronate + ?
-
no activity with enzyme acting on chondroitin, 50% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3 + H2O
D-glucuronate + ?
-
no activity with enzyme acting on chondroitin, 33% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAcbeta1-)2 + H2O
D-glucuronate + ?
-
66% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 90% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAcbeta1-)3 + H2O
D-glucuronate + ?
-
56% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 71% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GlcNAcbeta1-)3 + H2O
D-glucuronate + ?
-
81% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 73% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranoside + H2O
6-mercaptopurine + D-glucopyranose
-
-
-
-
?
1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosiduronamide + H2O
6-mercaptopurine + 1-deoxy-D-glucopyranosiduronamide
-
-
-
-
?
1-O-([4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamoyl)-beta-D-glucuronate + H2O
D-glucuronate + [4-[(10R,11S)-10-[[((2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamic acid
-
-
-
-
?
1-O-[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamic acid
-
-
-
-
?
1-O-[(4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamic acid
-
-
-
-
?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4,7-dimethyl-3,8,13-trioxo-11,13-diphenyl-2,9-dioxa-4,7,12-triazatridec-1-yl]phenyl beta-D-glucopyranosiduronate + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4,7-dimethyl-3,8-dioxo-2,9-dioxa-4,7-diazaundecan-11-oate
-
-
-
-
?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
-
-
-
-
?
4-([[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamate
-
-
-
-
?
4-([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-9-yl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + (4S)-4-ethyl-4-hydroxy-9-[(4-hydroxy-3-nitrobenzyl)oxy]-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione
-
-
-
-
?
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal + H2O
?
-
rate of hydrolysis is 106% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O
?
-
rate of hydrolysis is 117% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
4-Me-beta-GlcA-(1-6)-Gal + H2O
?
-
rate of hydrolysis is 62% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + beta-D-glucuronic acid
4-methylumbelliferyl-beta-D-glucuronic acid + H2O
4-methylumbelliferone + beta-D-glucuronic acid
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferol + D-glucuronate
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid
4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
-
preferred substrate
-
-
?
4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]-2-nitrophenyl beta-D-glucuronate + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-10-[(S)-(benzoylamino)(phenyl)methyl]-1-(4-hydroxy-3-nitrophenyl)-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
-
-
-
-
?
4-[([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)methyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-carboxylate
-
-
-
-
?
4-[([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamoyl]oxy)methyl]phenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxybenzyl [(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamate
-
-
-
-
?
4-[2-[1-(2-[2-[2-(beta-D-glucopyranuronosyloxy)ethoxy]ethoxy]ethyl)-1H-1,2,3-triazol-4-yl]-1-([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)ethyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 2-[2-(2-[4-[2-([[(3beta,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)-2-(4-hydroxy-3-nitrophenyl)ethyl]-1H-1,2,3-triazol-1-yl]ethoxy)ethoxy]ethyl beta-D-glucopyranosiduronic acid
-
-
-
-
?
5-bromo-4-chloro-3-indoyl-beta-D-glucuronide + H2O
5-bromo-4-chloro-1H-indol-3-ol + beta-D-glucuronate
-
-
-
-
?
ammonium 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosidurate + H2O
6-mercaptopurine + ammonium 1-deoxy-(6-thiopurinyl)-beta-D-glucopyranosiduronate
-
-
-
-
?
apigenin 7,4'-diglucuronide + H2O
apigenin 7-O-glucuronide + glucuronic acid
-
-
-
-
?
beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
D-glucuronate + beta-Gal(1-6)Gal
-
67.4% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
beta-GlcA(1-6)betaGal(1-6)Gal + H2O
D-glucuronate + beta-Gal(1-6)Gal
-
-
94.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
beta-GlcA-(1-3)-Gal + H2O
?
-
rate of hydrolysis is 2% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O
beta-Gal-(1-6)-Gal + beta-D-glucuronic acid
-
rate of hydrolysis is 67% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
beta-GlcA-(1-6)-Gal + H2O
D-galactose + beta-D-glucuronic acid
-
rate of hydrolysis is 76% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
carboxyumbelliferyl-beta-D-glucuronide + H2O
carboxyumbelliferone + beta-D-glucuronate
-
-
-
-
?
chondroitin + H2O
D-glucuronate + ?
-
MW 3500 Da: 23% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 41% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 8000 Da: 11% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 28% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 15000 Da: 6% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 9% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
curcumin beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
-
-
-
?
curcumin-beta-D-glucuronide + H2O
curcumin + beta-D-glucuronic acid
curcumin, despite low systemic bioavailability, may be enzymatically activated (deconjugated) within GUSB-enriched bone to exert protective effects, a metabolic process that can also contribute to bone-protective effects of other highly glucuronidated dietary polyphenols
-
-
?
GlcA-beta(1-6)Gal + H2O
D-glucuronate + D-galactopyranose
49.9% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
-
?
GlcAbeta(1-6)Gal + H2O
D-glucuronate + D-galactopyranose
-
85.7% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
-
?
GlcAbeta1-3GalNAc(4-OSO3-) + H2O
D-glucuronate + N-acetylamino-beta-D-galactopyranose 4-sulfate
-
no activity with enzyme acting on chondroitin, 4% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]-4'-O-glucuronide + H2O
?
-
-
-
-
?
methylumbelliferyl-beta-D-glucuronide + H2O
methylumbelliferone + beta-D-glucuronate
-
-
-
-
?
N-([[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy]carbonyl)-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-met + H2O
D-glucuronate + N-[[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl]-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide
-
-
-
-
?
o-nitrophenyl-beta-D-glucuronide + H2O
o-nitrophenol + D-glucuronic acid
-
-
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
D-glucuronate + ?
-
1% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 192% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronic acid
-
-
-
-
?
phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + beta-D-glucuronate
-
determination of specific activity in betaG-expressing tumour CT26 cells using radio-labeled substrate
-
-
?
phenolphthalein-beta-D-glucuronic acid + H2O
phenolphthalein + D-glucuronic acid
-
-
-
-
ir
quercetin 4'-O-beta-D-glucuronide + H2O
quercetin + beta-D-glucuronate
-
-
-
-
?
sodium (p-nitrophenyl beta-D-glucopyranoside)uronate + p-nitrophenyl alpha-D-galactopyranoside
p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside + p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside
-
the enzyme shows transglucuronidation activity: when (p-nitrophenyl beta-D-glucopyranoside)uronate is used as a donor and p-nitrophenyl alpha-D-galactopyranoside is used as an acceptor, p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside and p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside are synthesized in yields of 16% and 21% respectively
-
-
?
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
4-methyl-D-glucuronate + beta-Gal(1-6)Gal
-
-
42.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
4-methyl-D-glucuronate + beta-Gal(1-6)Gal
-
4.8% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + beta-D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + beta-D-glucuronic acid
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
-
-
-
?
4-methylumbelliferyl beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
DQ459484
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
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4-nitrophenol beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenol beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
Aspergillus oryzae Li-3
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4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
32% of the activity with 4-nitrophenyl beta-D-glucuronide
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4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
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4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
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4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
32% of the activity with 4-nitrophenyl beta-D-glucuronide
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4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid
4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid
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4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid
4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid
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the enzyme shows transglucuronidation activity: when sodium (p-nitrophenyl beta-D-glucopyranoside)uronate is used as an acceptor and as a donor, sodium (sodium beta-D-glucopyranosyluronate)-(1-3)-(p-nitrophenyl beta-D-glucopyranosid)uronate and sodium (sodium beta-D-glucopyranosyluronate)-(1-2)-(p-nitrophenyl beta-D-glucopyranosid)uronate are obtained in yields of 18% and 15% respectively
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4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
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4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
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4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
low activity
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4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
low activity
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4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
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4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
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4-nitrophenyl beta-D-glucuronic acid + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronic acid + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
A0A0H1IHR6
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
best substrate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
best substrate
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
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4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
the enzyme has a moderately broad specificity, hydrolysing a range of 4-nitrophenyl glycoside substrates, but has greatest activity on 4-nitrophenyl beta-D-glucuronide. The catalytic nucleophile is Glu476 within the sequence VTEFGAD
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baicalin + H2O
baicalein + beta-D-glucuronate
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a major flavonoid derived from the root of Scutellaria baicalensis
productivity of 73%
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glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + H2O
glycyrrhetic acid + D-glucuronate
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
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glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + H2O
glycyrrhetic acid + D-glucuronate
Aspergillus oryzae Li-3
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
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glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
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specific substrate of isozymes I and II
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glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
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specific substrate of isozymes I and II
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glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
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glycyrrhizin + H2O
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + D-glucuronate
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
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glycyrrhizin + H2O
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide + D-glucuronate
Aspergillus oryzae Li-3
the enzyme shows similar activities toward both glycyrrhizin and glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
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p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucuronic acid
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p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucuronic acid
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p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucuronic acid
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p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
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p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
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phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
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phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
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phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
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phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
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phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
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phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
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phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
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wogonin 7-O-beta-D-glucuronide + H2O
wogonin + beta-D-glucuronate
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wogonin 7-O-beta-D-glucuronide + H2O
wogonin + beta-D-glucuronate
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additional information
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activity is slightly inducible by Met-Gly
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additional information
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enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose
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additional information
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the enzyme does not show strict specificity toward the aglycone moiety and exhibits activity toward all the natural and artificial substrates. Strict glycan specificity and hydrolyzes only the artificial substrate containing glucuronide groups
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additional information
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Aspergillus oryzae Li-3
the enzyme does not show strict specificity toward the aglycone moiety and exhibits activity toward all the natural and artificial substrates. Strict glycan specificity and hydrolyzes only the artificial substrate containing glucuronide groups
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additional information
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activity is not inducible by Met-Gly
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additional information
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role in physiology, in tissues, in body fluids
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additional information
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the potent beta-glucuronidase activity caused by the glucuronic acid conjugates from xenobiotics and endogenous compounds is a prime factor in the etiology of colon cancer
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additional information
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when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed
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additional information
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when beta-glucuronidase producing bacteria infect the bile, the pH of the bile becomes raised, the high pH and bile induce the enzyme, and then bilirubin gallstones can be easily formed
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additional information
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substrate specificity with flavonoids and phytoestrogens of crude and purified enzyme from digestive juice, overview
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additional information
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transfer of beta-glucuronosyl residues from aryl and alicyclic glucuronides to aliphatic alcohols and glycerols
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additional information
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the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its retinoyl ester hydrolase activity
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additional information
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enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose
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additional information
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enzyme additionally catalyzes the transglycosylation of glucuronic acid residues from 4-nitrophenyl beta-D-glucuronic acid to various monosaccharide acceptors such as glucose, galactose, and xylose
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additional information
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no activity with enzyme acting on chondroitin: N-acetylhyalobiuronic acid, N-acetylchondrosine, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2, GlcAbeta1-3galNAc(4-OSO3-), (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2. No activity with enzyme acting on p-nitrophenyl-beta-D-glucuronide: N-acetylhyalobiuronic acid, N-acetylchondrosine
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additional information
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the enzyme is involved in glycosaminoglycan metabolism
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additional information
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activity is not inducible by Met-Gly
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additional information
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activity is not inducible by Met-Gly
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additional information
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no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide
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additional information
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no activity of isozymes I and II with 4-nitrophenyl beta-D-glucuronide, baicalin, and 18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide
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