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Literature summary for 3.2.1.31 extracted from
- Stable expression of Escherichia coli beta-glucuronidase A (GusA) in Giardia lamblia: application to high-throughput drug susceptibility testing (2009), J. Antimicrob. Chemother., 64, 1187-1191.
Application
| Application | Comment | Organism |
|---|---|---|
| drug development | stable expression of GusA in Gardia lamblia strain WB C6 for establishing a system to test enzyme susceptibility to the anti-giardial drugs nitazoxanide and metronidazole, overview | Escherichia coli |
| molecular biology | stable expression of GusA in Gardia lamblia strain WB C6 for establishing a system to test enzyme susceptibility to the anti-giardial drugs nitazoxanide and metronidazole, overview | Escherichia coli |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His-tagged GusA in Escherichia coli strain TOP10 and BL21, stable expression of GusA in Gardia lamblia strain WB C6 trophozoites, under control of the promoter from the glutamate dehydrogenase, gdh, gene, for establishing a system to test enzyme susceptibility to the anti-giardial drugs nitazoxanide and metronidazole, overview | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl D-glucuronide + H2O | - |
Escherichia coli | 4-nitrophenol + D-glucuronate | - |
? |  |
| 5-bromo-4-chloro-3-indoyl-beta-D-glucuronide + H2O | - |
Escherichia coli | 5-bromo-4-chloro-1H-indol-3-ol + beta-D-glucuronate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-glucuronidase A | - |
Escherichia coli |
| GusA | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Escherichia coli |
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