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Literature summary for 3.2.1.31 extracted from

  • Russell, W.M.; Klaenhammer, T.E.
    Identification and cloning of gusA, encoding a new beta-glucuronidase from Lactobacillus gasseri ADH (2001), Appl. Environ. Microbiol., 67, 1253-1261.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
GusA is expressed from the cloned gusA gene by a constitutive promoter in Lactobacillus gasseri ATCC 33323 and by a regulated promoter in Escherichia coli Lactobacillus gasseri

Inhibitors

Inhibitors Comment Organism Structure
saccharic acid 1,4-lactone 80% inhibition by 0.5 mM, 88% inhibition by 1.0 mM Lactobacillus gasseri

Organism

Organism UniProt Comment Textmining
Lactobacillus gasseri Q9AHJ8
-
-
Lactobacillus gasseri ADH Q9AHJ8
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
p-nitrophenyl-beta-D-glucuronide + H2O
-
Lactobacillus gasseri p-nitrophenol + beta-D-glucuronic acid
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
-
Lactobacillus gasseri ADH p-nitrophenol + beta-D-glucuronic acid
-
?

Synonyms

Synonyms Comment Organism
GusA
-
Lactobacillus gasseri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Lactobacillus gasseri

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 75 37°C: about 60% of maximal activity, 75°C: about 90% of maximal activity Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Lactobacillus gasseri

pH Range

pH Minimum pH Maximum Comment Organism
4 7 pH 4.0: about 50% of maximal activity, pH 7.0: 25% of maximal activity Lactobacillus gasseri