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3.2.1.31: beta-glucuronidase

This is an abbreviated version!
For detailed information about beta-glucuronidase, go to the full flat file.

Word Map on EC 3.2.1.31

Reaction

a beta-D-glucuronoside
+
H2O
=
D-glucuronate
 +
an alcohol

Synonyms

beta-D-glucuronidase, Beta-D-glucuronoside glucuronosohydrolase, Beta-G1, beta-Gluc, beta-glucuronidase, beta-glucuronidase A, betaG, BLG, exo-beta-D-glucuronidase, exo-beta-glucuronidase, GlcAase, glucuronidase, beta-glucuronide glucuronohydrolase, GUR, GUS, Gus2, GusA, GusB, hGUSB, ketodase, More, PGUS, TM1062, TmGUSI, UidA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.31 beta-glucuronidase

Cloned

Cloned on EC 3.2.1.31 - beta-glucuronidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
beta-D-glucuronidase gene derived from Penicillium purpurogenum Li-3 is expressed in Pichia pastoris GS115 in two different environments of low-shear modeled microgravity (LSMMG) and normal gravity (NG)
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree
expressed in 3T3 fibroblasts and EJ carcinoma cells
expressed in Escherichia coli
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli strain JM109
-
expressed in Escherichia coli strain Origami B(DE3)
-
expression in both Escherichia coli and Arabidopsis thaliana. The enzyme expressed in transformed Escherichia coli cells exhibits maximum hydrolytic activity at 65°C and pH 6.5 and retains more than 80% activity after incubation at 85°C for 30 min. Activity in transgenic Arabidopsis thaliana plants containing TmGUSI is also stable over the temperature range 65-80°C
DQ459484
expression in Escherichia coli
expression in Escherichia coli. Although Escherichia coli possesses an endogenous beta-glucuronidase activity, a short treatment at 80°C of the crude extract causes the precipitation of most Escherichia coli protein, resulting in an enrichment of the Sulfolobus beta-glucuronidase
expression in Pichia pastoris
expression in transgenic Arabidopsis
-
expression of red fluorescent protein, RFP, fusion Gus in COS-7 cells, coexpression with GFP-tagged esterase 22
-
expression of the protein His6-tagged at its N terminus in Escherichia coli
expression vectors pMCG-GLUC and pMCG-dGLUC containing cDNA coding for human beta-glucuronidase and a C-terminal-truncated form of beta-glucuronidase respectively are transfected into BHK cells. The stable clones B702 expressing wild-type and B688 expressing mutant beta-glucuronidase. In the mutant enzyme a stop codon is introduced after the CAC/His-637 codon, thereby eliminating 14 amino acids from the C-terminus
-
gene gusA, expression of His-tagged wild-type and mutant enzymes in Escherichia coli
-
gene TM1062, DNA and amino acid sequence determination and analysis, expression of His6-tagged enzyme in Escherichia coli strain GMS407
gene uidA, DNA and amino acid sequence determination and analysis, genetic organization, expression analysis, expression in Escherichia coli strain TG1 mutant with inactivated endogenous enzyme activity, complementation of enzyme-deficient Escherichia coli strain
GUS, DNA and amino acid sequence determination, analysis and comparison, overexpression of the His-tagged enzyme in Escherichia coli strain GMS407
-
GusA is expressed from the cloned gusA gene by a constitutive promoter in Lactobacillus gasseri ATCC 33323 and by a regulated promoter in Escherichia coli
mutants expressed in Escherichia coli DH5alpha cells
-
over-expressed in wheat plants
-
recombinant expression of His-tagged GusA in Escherichia coli strain TOP10 and BL21, stable expression of GusA in Gardia lamblia strain WB C6 trophozoites, under control of the promoter from the glutamate dehydrogenase, gdh, gene, for establishing a system to test enzyme susceptibility to the anti-giardial drugs nitazoxanide and metronidazole, overview
-
wild-type and mutant enzymes expressed COS cells and GUSB-deficient fibroblasts from the SV40 late promoter in vector pJC119
-