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3.2.1.31: beta-glucuronidase

This is an abbreviated version!
For detailed information about beta-glucuronidase, go to the full flat file.

Word Map on EC 3.2.1.31

Reaction

a beta-D-glucuronoside
+
H2O
=
D-glucuronate
 +
an alcohol

Synonyms

beta-D-glucuronidase, Beta-D-glucuronoside glucuronosohydrolase, Beta-G1, beta-Gluc, beta-glucuronidase, beta-glucuronidase A, betaG, BLG, exo-beta-D-glucuronidase, exo-beta-glucuronidase, GlcAase, glucuronidase, beta-glucuronide glucuronohydrolase, GUR, GUS, Gus2, GusA, GusB, hGUSB, ketodase, More, PGUS, TM1062, TmGUSI, UidA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.31 beta-glucuronidase

KM Value

KM Value on EC 3.2.1.31 - beta-glucuronidase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.49
1-Deoxy-1-(6-thiopurinyl)-beta-D-glucopyranoside
-
-
9.25
1-Deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosiduronamide
-
-
1.4
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal
-
pH 4.6, 37°C
1.9
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal
-
pH 4.6, 37°C
3.6
4-Me-beta-GlcA-(1-6)-Gal
-
pH 4.6, 37°C
0.041 - 1.3
4-methylumbelliferyl beta-D-glucuronide
1.25
4-methylumbelliferyl-beta-D-glucuronic acid
pH and temperature not specified in the publication
0.041 - 2.76
4-methylumbelliferyl-beta-D-glucuronide
3.06
4-nitrophenyl beta-D-galactosiduronide
0.0304 - 145
4-nitrophenyl beta-D-glucoside
0.0046 - 0.36
4-nitrophenyl beta-D-glucuronide
0.2
4-nitrophenyl beta-D-xylopyranoside
-
wild-type enzyme
0.208 - 1.4
4-nitrophenyl-beta-D-glucuronide
0.132
Ammonium 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosidurate
-
-
0.53
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal
-
pH 4.6, 37°C
0.71
beta-GlcA-(1-6)-Gal
-
pH 4.6, 37°C
0.91
carboxyumbelliferyl-beta-D-glucuronide
-
pH 6.5, 38°C, Vmax: 0.041 mM/min
6.3
chondroitin
-
pH 5.0, 37°C
0.272
daidzein 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.266
equol 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.2
estriol 3-glucuronide
-
estriol-16-alpha-glucuronide
0.378 - 0.422
GlcAbeta(1-6)Gal
0.91 - 1.1
glycyrrhetinic acid 3-O-mono-beta-D-glucuronide
0.78 - 7.72
glycyrrhizin
0.025
kaempferol 3-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.007
luteolin 7-O-diglucuronide 4'-O-glucuronide
-
-
0.065
methylumbelliferyl-beta-D-glucuronide
-
pH 6.5, 38°C, Vmax: 0.026 mM/min
0.02
oestrone-3-glucuronide
-
-
0.13 - 2.9
p-nitrophenyl-beta-D-glucuronide
0.018 - 3.08
phenolphthalein beta-D-glucuronide
2.907
phenolphthalein-beta-D-glucuronic acid
-
pH 5.0, 52°C
0.048
quercetin 4'-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.237
quercetin 7-O-beta-D-glucuronide
-
pH 7.2, 37°C
0.167
quercetin-3-O-beta-D-glucuronide
-
pH 7.2, 37°C
additional information
additional information
-