Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.31 extracted from

  • Krahulec, J.; Krahulcova, J.
    Characterization of the new beta-glucuronidase from Streptococcus equi subsp. zooepidemicus (2007), Appl. Microbiol. Biotechnol., 74, 1016-1022.
    View publication on PubMed
Search for more literature for 3.2.1.31

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain Origami B(DE3) Streptococcus equi

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Streptococcus equi 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Na+, K+, Mg2+, Mn2+, and Ca2+ increase the activity but with no significance Streptococcus equi

Organism

Organism UniProt Comment Textmining
Streptococcus equi
-
 subspecies Streptococcus equi zooepidemicus
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptococcus equi

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
117
-
 purified recombinant enzyme, at pH 7.0 and 37°C Streptococcus equi
160
-
 purified recombinant enzyme, at pH 5.6 and 37°C Streptococcus equi
270
-
 purified recombinant enzyme, at pH 7.0 and 52°C Streptococcus equi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-glucuronide + H2O
-
 Streptococcus equi 4-nitrophenol + D-glucuronic acid
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
52
-
-
 Streptococcus equi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Streptococcus equi