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3.2.1.31: beta-glucuronidase

This is an abbreviated version!
For detailed information about beta-glucuronidase, go to the full flat file.

Word Map on EC 3.2.1.31

Reaction

a beta-D-glucuronoside
+
H2O
=
D-glucuronate
 +
an alcohol

Synonyms

beta-D-glucuronidase, Beta-D-glucuronoside glucuronosohydrolase, Beta-G1, beta-Gluc, beta-glucuronidase, beta-glucuronidase A, betaG, BLG, exo-beta-D-glucuronidase, exo-beta-glucuronidase, GlcAase, glucuronidase, beta-glucuronide glucuronohydrolase, GUR, GUS, Gus2, GusA, GusB, hGUSB, ketodase, More, PGUS, TM1062, TmGUSI, UidA

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.31 beta-glucuronidase

Purification

Purification on EC 3.2.1.31 - beta-glucuronidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 different exo-beta-glucuronidases: a beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide and a beta-glucuronidase acting on chondroitin
-
2 forms
2 forms, partial
-
3 forms
Blue Sepharose FF column chromatography, phenyl-Sepharose high-Sub FF column chromatography, DEAE-Sephacel column chromatography
-
DEAE Sepharose Fast Flow column chromatography and polyethyleneimine gel filtration
-
from liver microsomes by calcium precipitation, immunoaffinity chromatography, preparative PAGE, gel filtration, and anion exchange chromatography to homogeneity
-
lactamyl-Sepharose column chromatography
-
native enzyme from digestive juice by ion exchange chromatography on a resin containing a polymeric sorbent
-
native enzyme from eggs, by ammonium sulfate fractionation, anion exchange chromatography, and heparin affinity chromatography
-
native isozymes I and II to homogeneity by ammonium sulfate fractionation, hydrophobic interaction, ion exchange, and hydroxyapatite chromatographies, and glycyrrhizin affinity chromatography
-
Ni2+-affinity chromatography, anion-exchange chromatography, and gel filtration
nickel chelate affinity chromatography
-
partial
-
recombinant GUS from Escherichia coli strain GMS407
-
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography to homogeneity
-
recombinant His6-tagged enzyme from Escherichia coli strain GMS407 by metal affinity chromatography to homogeneity