Information on EC 3.2.1.31 - beta-glucuronidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.31
-
RECOMMENDED NAME
GeneOntology No.
beta-glucuronidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a beta-D-glucuronoside + H2O = D-glucuronate + an alcohol
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
exoglycosidic
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
beta-D-glucuronide and D-glucuronate degradation
-
-
degradation of sugar acids
-
-
Drug metabolism - other enzymes
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-
Flavone and flavonol biosynthesis
-
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Glycosaminoglycan degradation
-
-
luteolin triglucuronide degradation
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-
Metabolic pathways
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Pentose and glucuronate interconversions
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Porphyrin and chlorophyll metabolism
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Starch and sucrose metabolism
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SYSTEMATIC NAME
IUBMB Comments
beta-D-glucuronoside glucuronosohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9001-45-0
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain GV3101
-
-
Manually annotated by BRENDA team
strain GV3101
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
HGU-3
-
-
Manually annotated by BRENDA team
strain K12
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain RO1
-
-
Manually annotated by BRENDA team
strain RO1
-
-
Manually annotated by BRENDA team
strain ADH
SwissProt
Manually annotated by BRENDA team
strain ATCC33323
SwissProt
Manually annotated by BRENDA team
strain LD 106
-
-
Manually annotated by BRENDA team
strain LD 106
-
-
Manually annotated by BRENDA team
cultivar Georgia
-
-
Manually annotated by BRENDA team
marine mollusc
-
-
Manually annotated by BRENDA team
fragment
UniProt
Manually annotated by BRENDA team
-
UniProt
Manually annotated by BRENDA team
no activity in Escherichia adecarboxylata
-
-
-
Manually annotated by BRENDA team
no activity in Escherichia blattae
-
-
-
Manually annotated by BRENDA team
no activity in Escherichia fergusonii
-
-
-
Manually annotated by BRENDA team
no activity in Escherichia hermannii
-
-
-
Manually annotated by BRENDA team
no activity in Escherichia vulneris
-
-
-
Manually annotated by BRENDA team
cultivar Pusa Basmati
-
-
Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain E1, isolated from human intestinal microflora, gene uidA
SwissProt
Manually annotated by BRENDA team
strain E1, isolated from human intestinal microflora, gene uidA
SwissProt
Manually annotated by BRENDA team
rye
-
-
Manually annotated by BRENDA team
RLH1
UniProt
Manually annotated by BRENDA team
RLH1
UniProt
Manually annotated by BRENDA team
subspecies Streptococcus equi zooepidemicus
-
-
Manually annotated by BRENDA team
part of the normal flora of the respiratory and urogenital tracts of equines
UniProt
Manually annotated by BRENDA team
strain LJ-22, isolated from human intestinal microflora, two isozymes I and II
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-
Manually annotated by BRENDA team
strain LJ-22, isolated from human intestinal microflora, two isozymes I and II
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Manually annotated by BRENDA team
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-
-
Manually annotated by BRENDA team
strain MSB8, gene TM1062
SwissProt
Manually annotated by BRENDA team
cultivar Sathi
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-
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
-
beta-glucuronidase, an acid hydrolase that deconjugates glucuronides, may increase cancer risk
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1S)-1-(chloromethyl)-3-([5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]carbonyl)-2,3-dihydro-1H-benzo[e]indol-5-yl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + [(1S)-1-(chloromethyl)-5-hydroxy-1,2-dihydro-3H-benzo[e]indol-3-yl][5-[2-(dimethylamino)ethoxy]-1H-indol-2-yl]methanone
show the reaction diagram
-
-
-
-
?
(1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-{[({2-[({[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy}carbonyl)amino]-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl}oxy)carbonyl]amino}-alpha-D-lyxo-hexopyranoside + H2O
D-glucuronate + (1S,3S)-3,12-dihydroxy-3-(hydroxyacetyl)-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 2,3,6-trideoxy-3-[({[2-({[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl}amino)-5-({[(2-{[4-({[(pyridin-3-ylmethoxy)carbonyl]amino}methyl)benzoyl]amino}phenyl)carbamoyl]oxy}methyl)benzyl]oxy}carbonyl)amino]-alpha-D-lyxo-hexopyranoside
show the reaction diagram
-
-
-
-
?
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2 + H2O
D-glucuronate + ?
show the reaction diagram
-
no activity with enzyme acting on chondroitin, 142% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3 + H2O
D-glucuronate + ?
show the reaction diagram
-
no activity with enzyme acting on chondroitin, 63% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2 + H2O
D-glucuronate + ?
show the reaction diagram
-
no activity with enzyme acting on chondroitin, 50% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3 + H2O
D-glucuronate + ?
show the reaction diagram
-
no activity with enzyme acting on chondroitin, 33% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAcbeta1-)2 + H2O
D-glucuronate + ?
show the reaction diagram
-
66% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 90% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GalNAcbeta1-)3 + H2O
D-glucuronate + ?
show the reaction diagram
-
56% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 71% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
(4GlcAbeta1-3GlcNAcbeta1-)3 + H2O
D-glucuronate + ?
show the reaction diagram
-
81% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 73% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranoside + H2O
6-mercaptopurine + D-glucopyranose
show the reaction diagram
-
-
-
-
-
1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosiduronamide + H2O
6-mercaptopurine + 1-deoxy-D-glucopyranosiduronamide
show the reaction diagram
-
-
-
-
-
1-O-([4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamoyl)-beta-D-glucopyranur + H2O
D-glucuronate + [4-[(10R,11S)-10-[[((2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl]carbamic acid
show the reaction diagram
-
-
-
-
?
1-O-[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamic acid
show the reaction diagram
-
-
-
-
?
1-O-[(4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamoyl]-beta-D-glucopyranuronic acid + H2O
D-glucuronate + (4-[[([[(1S,2R)-1-(benzoylamino)-3-[[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]-3-oxo-1-phenylpropan-2-yl]oxy]carbonyl)oxy]methyl]phenyl)carbamic acid
show the reaction diagram
-
-
-
-
?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4,7-dimethyl-3,8,13-trioxo-11,13-diphenyl-2,9-dioxa-4,7,12-triazatridec-1-yl]phenyl beta-D-glucopyranosiduro + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4,7-dimethyl-3,8-dioxo-2,9-dioxa-4,7-diazaundecan-11-oate
show the reaction diagram
-
-
-
-
?
2-amino-4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]phenyl beta-D-glucopyranosiduronic + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-1-(3-amino-4-hydroxyphenyl)-10-[(S)-(benzoylamino)(phenyl)methyl]-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
show the reaction diagram
-
-
-
-
?
4-([[(2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamoyl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (2-[[4-(acetylamino)benzoyl]amino]phenyl)carbamate
show the reaction diagram
-
-
-
-
?
4-([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-9-yl]oxy]methyl)-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + (4S)-4-ethyl-4-hydroxy-9-[(4-hydroxy-3-nitrobenzyl)oxy]-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinoline-3,14(4H,12H)-dione
show the reaction diagram
-
-
-
-
?
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal + H2O
?
show the reaction diagram
-
rate of hydrolysis is 106% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O
?
show the reaction diagram
-
rate of hydrolysis is 117% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
4-Me-beta-GlcA-(1-6)-Gal + H2O
?
show the reaction diagram
-
rate of hydrolysis is 62% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
4-methyl-beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
4-methyl-D-glucuronate + beta-Gal(1-6)Gal
show the reaction diagram
4-methylumbelliferyl beta-D-glucuronide
4-methylumbelliferone + D-glucuronic acid
show the reaction diagram
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferol + D-glucuronate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-beta-D-glucuronide + H2O
4-methylumbelliferone + D-glucuronic acid
show the reaction diagram
4-nitrophenyl beta-D-galactosiduronide + H2O
4-nitrophenol + beta-D-galactosiduronic acid
show the reaction diagram
32% of the activity with 4-nitrophenyl beta-D-glucuronide
-
-
?
4-nitrophenyl beta-D-glucopyranosiduronic acid + 4-nitrophenyl beta-D-glucopyranosiduronic acid
4-nitrophenol + 4-nitrophenyl 3-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid + 4-nitrophenyl 2-O-beta-D-glucopyranuronosyl-beta-D-glucopyranosiduronic acid
show the reaction diagram
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose
show the reaction diagram
4-nitrophenyl beta-D-glucuronic acid + H2O
4-nitrophenol + D-glucuronic acid
show the reaction diagram
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronic acid
show the reaction diagram
4-nitrophenyl beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronate
show the reaction diagram
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylopyranose
show the reaction diagram
-
preferred substrate
-
-
?
4-nitrophenyl D-glucuronide + H2O
4-nitrophenol + D-glucuronate
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-beta-D-glucuronide + H2O
4-nitrophenol + beta-D-glucuronate
show the reaction diagram
4-nitrophenyl-beta-D-glucuronide + H2O
4-nitrophenol + D-glucuronic acid
show the reaction diagram
4-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronic acid
show the reaction diagram
4-phenolphthaleinyl beta-D-glucuronide + H2O
4-phenolphthalein + beta-D-glucuronic acid
show the reaction diagram
-
-
-
-
?
4-[(10R,11S)-10-([[(2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl]oxy]carbonyl)-4-methyl-3,8,13-trioxo-11,13-diphenyl-2,7,9-trioxa-4,12-diazatridec-1-yl]-2-nitrophenyl beta-D-glucopyranosiduronic + H2O
D-glucuronate + (2alpha,5beta,7beta,13alpha)-4,10-bis(acetyloxy)-2-(benzoyloxy)-7-hydroxy-9-oxo-5,20-epoxytax-11-en-13-yl (10R)-10-[(S)-(benzoylamino)(phenyl)methyl]-1-(4-hydroxy-3-nitrophenyl)-4-methyl-3,8-dioxo-2,7,9-trioxa-4-azaundecan-11-oate
show the reaction diagram
-
-
-
-
?
4-[([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)methyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxy-3-nitrobenzyl (3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-carboxylate
show the reaction diagram
-
-
-
-
?
4-[([[(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamoyl]oxy)methyl]phenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 4-hydroxybenzyl [(4S)-4-ethyl-4-hydroxy-3,14-dioxo-3,4,12,14-tetrahydro-1H-pyrano[3',4':6,7]indolizino[1,2-b]quinolin-10-yl]carbamate
show the reaction diagram
-
-
-
-
?
4-[2-[1-(2-[2-[2-(etab-D-glucopyranuronosyloxy)ethoxy]ethoxy]ethyl)-1H-1,2,3-triazol-4-yl]-1-([[(3b,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)ethyl]-2-nitrophenyl beta-D-glucopyranosiduronic acid + H2O
D-glucuronate + 2-[2-(2-[4-[2-([[(3beta,22S,23R)-3-hydroxy-17,23-epoxyveratraman-28-yl]carbonyl]oxy)-2-(4-hydroxy-3-nitrophenyl)ethyl]-1H-1,2,3-triazol-1-yl]ethoxy)ethoxy]ethyl beta-D-glucopyranosiduronic acid
show the reaction diagram
-
-
-
-
?
5-bromo-4-chloro-3-indoyl-beta-D-glucuronide + H2O
5-bromo-4-chloro-3-indole beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
alpha-L-arabinosidase-treated-arabinogalactan-protein + H2O
?
show the reaction diagram
-
-
-
-
?
ammonium 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosidurate + H2O
6-mercaptopurine + ammonium 1-deoxy-(6-thiopurinyl)-beta-D-glucopyranosiduronate
show the reaction diagram
-
-
-
-
-
androstendione-enol-beta-D-glucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
apigenin 7,4'-diglucuronide + H2O
apigenin 7-O-glucuronide + glucuronic acid
show the reaction diagram
-
-
-
-
-
baicalein 7-O-beta-D-glucuronide + H2O
baicalein + beta-D-glucuronic acid
show the reaction diagram
baicalin + H2O
baicalein + beta-D-glucuronate
show the reaction diagram
baicalin + H2O
baicalein + beta-D-glucuronic acid
show the reaction diagram
baicalin + H2O
baicalein + D-glucuronate
show the reaction diagram
beta-GlcA(1-6)beta-Gal(1-6)Gal + H2O
D-glucuronate + beta-Gal(1-6)Gal
show the reaction diagram
-
67.4% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
beta-GlcA(1-6)betaGal(1-6)Gal + H2O
D-glucuronate + beta-Gal(1-6)Gal
show the reaction diagram
-
-
94.6% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
?
beta-GlcA-(1-3)-Gal + H2O
?
show the reaction diagram
-
rate of hydrolysis is 2% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal + H2O
beta-Gal-(1-6)-Gal + beta-D-glucuronic acid
show the reaction diagram
-
rate of hydrolysis is 67% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
beta-GlcA-(1-6)-Gal + H2O
D-galactose + beta-D-glucuronic acid
show the reaction diagram
-
rate of hydrolysis is 76% of the with p-nitrophenyl-beta-D-glucuronide
-
-
?
bilirubin diglucuronide + H2O
bilirubin + D-glucuronate
show the reaction diagram
carboxyumbelliferyl-beta-D-glucuronide + H2O
carboxyumbelliferone + beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
chondroitin + H2O
D-glucuronate + ?
show the reaction diagram
-
MW 3500 Da: 23% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 41% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 8000 Da: 11% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 28% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 15000 Da: 6% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 9% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
daidzein 7-O-beta-D-glucuronide + H2O
daidzein + D-glucuronate
show the reaction diagram
-
-
-
-
?
dermatan sulfate + H2O
?
show the reaction diagram
-
-
-
-
-
equol 7-O-beta-D-glucuronide + H2O
equol + D-glucuronate
show the reaction diagram
-
-
-
-
?
GlcA-beta(1-6)Gal + H2O
D-glucuronate + D-galactopyranose
show the reaction diagram
49.9% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
-
?
GlcAbeta(1-6)Gal + H2O
D-glucuronate + D-galactopyranose
show the reaction diagram
-
85.7% of the activity with 4-nitrophenyl beta-D-glucuronic acid
-
-
?
GlcAbeta1-3GalNAc(4-OSO3-) + H2O
D-glucuronate + N-acetylamino-beta-D-galactopyranose 4-sulfate
show the reaction diagram
-
no activity with enzyme acting on chondroitin, 4% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
glucuronic acid 1-phosphate + H2O
glucuronic acid + phosphate
show the reaction diagram
-
-
-
-
-
glycyrrhizin + H2O
18beta-glycyrrhetinic acid-3-O-beta-D-glucuronide + beta-D-glucuronic acid
show the reaction diagram
heparan sulfate + H2O
?
show the reaction diagram
-
-
-
-
-
kaempferol 3-O-beta-D-glucuronide + H2O
kaempferol + D-glucuronate
show the reaction diagram
-
-
-
-
?
luteolin 3'-O-beta-glucuronide + H2O
luteolin + D-glucuronate
show the reaction diagram
luteolin 7-O-glucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] + H2O
?
show the reaction diagram
-
-
-
-
-
luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide]-4'-O-glucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
luteolin triglucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
methylumbelliferyl-beta-D-glucuronide + H2O
methylumbelliferone + beta-D-glucuronate
show the reaction diagram
-
-
-
-
?
N-([[4-(beta-D-glucopyranuronosyloxy)-3-nitrobenzyl]oxy]carbonyl)-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-met + H2O
D-glucuronate + N-[[(4-hydroxy-3-nitrobenzyl)oxy]carbonyl]-N-methyl-L-valyl-N-[(3R,4S,5S)-1-[(2S)-2-[(1R,2R)-3-[[(1S,2R)-1-hydroxy-1-phenylpropan-2-yl]amino]-1-methoxy-2-methyl-3-oxopropyl]pyrrolidin-1-yl]-3-methoxy-5-methyl-1-oxoheptan-4-yl]-N-methyl-L-valinamide
show the reaction diagram
-
-
-
-
?
naphthol AS-BI-D-glucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
o-nitrophenyl-beta-D-glucuronide + H2O
o-nitrophenol + D-glucuronic acid
show the reaction diagram
oestrone 3-glucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
oroxylin 7-O-beta-D-glucuronide + H2O
oroxylin + D-glucuronate
show the reaction diagram
p-nitrophenyl-beta-D-glucuronide + H2O
D-glucuronate + ?
show the reaction diagram
-
1% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 192% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide
-
-
?
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + beta-D-glucuronic acid
show the reaction diagram
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronate
show the reaction diagram
p-nitrophenyl-beta-D-glucuronide + H2O
p-nitrophenol + D-glucuronic acid
show the reaction diagram
phenolphthalein beta-D-glucuronide + H2O
phenolphthalein + beta-D-glucuronate
show the reaction diagram
-
determination of specific activity in betaG-expressing tumour CT26 cells using radio-labeled substrate
-
-
?
phenolphthalein-beta-D-glucuronic acid + H2O
phenolphthalein + D-glucuronic acid
show the reaction diagram
-
-
-
-
ir
phenolphthalein-beta-D-glucuronide + H2O
phenolphthalein + D-glucuronic acid
show the reaction diagram
quercetin 3-O-beta-D-glucuronide + H2O
quercetin + D-glucuronate
show the reaction diagram
-
-
-
-
?
quercetin 7-O-beta-D-glucuronide + H2O
quercetin + D-glucuronate
show the reaction diagram
-
-
-
-
?
quercetin-4'-O-glucuronide + H2O
quercetin + D-glucuronate
show the reaction diagram
-
-
-
-
?
retinyl-beta-glucuronide + H2O
retinol + D-glucuronide
show the reaction diagram
-
-
-
-
?
sodium (p-nitrophenyl beta-D-glucopyranoside)uronate + p-nitrophenyl alpha-D-galactopyranoside
p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside + p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside
show the reaction diagram
-
the enzyme shows transglucuronidation activity: when (p-nitrophenyl beta-D-glucopyranoside)uronate is used as a donor and p-nitrophenyl alpha-D-galactopyranoside is used as an acceptor, p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-4)-alpha-D-galactopyranoside and p-nitrophenyl (sodium beta-D-glucopyranosyluronate)-(1-2)-alpha-D-galactopyranoside are synthesized in yields of 16% and 21% respectively
-
-
-
wogonin + H2O
wogonin aglycon + beta-D-glucuronate
show the reaction diagram
wogonin 7-O-beta-D-glucuronide + H2O
wogonin + D-glucuronate
show the reaction diagram
-
-
-
-
?
wogonin glucuronide + H2O
wogonin + D-glucuronate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
baicalin + H2O
baicalein + beta-D-glucuronic acid
show the reaction diagram
-
-
-
-
?
dermatan sulfate + H2O
?
show the reaction diagram
-
-
-
-
-
heparan sulfate + H2O
?
show the reaction diagram
-
-
-
-
-
luteolin triglucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
oestrone 3-glucuronide + H2O
?
show the reaction diagram
-
-
-
-
-
retinyl-beta-glucuronide + H2O
retinol + D-glucuronide
show the reaction diagram
-
-
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
activity enhanced
K+
activity enhanced
Mn2+
activity enhanced
Na+
activity enhanced
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,1'-(3-methyl-4-phenylthieno[2,3-b]thiophene-2,5-diyl)bis(4,4,4-triethoxybut-2-en-1-one)
-
-
-
1,4-D-glucarolactone
2-deoxy-2-fluoro-beta-D-glucosyluronic acid fluoride
inactivation the recombinant His6-tagged enzyme
4-chloromercuriphenylsulfonic acid
-
inhibition of isozymes I and II
ascorbic acid
Ca2+
-
0.3 mM, 15% inhibition
CaCl2
-
2 mM, 14.8% inhibition
Citric acid
-
-
D-glucaric acid 1,4-lactone
-
5 mM, 30% inhibition
D-glucaro-1,4-lactone
-
-
D-glucose
-
10 mM, about 15% inhibition
D-glucuronate
-
IC50: 7.5 mM, competitive
D-glucuronic acid
-
10 mM, about 15% inhibition
D-saccharic acid 1,4-lactone
D-saccharic acid 1,4-lactone monohydrate
-
a betaG-specific inhibitor
D-saccharic acid-1,4-lactone
-
-
delta-D-glucuronolactone
-
-
estradiol 3-glucuronide
-
-
Fe2+
-
inhibits 29% at 1 mM, 71% at 100 mM
FeCl2
-
2 mM,33.1% inhibition
fucoxanthin
glucuronic acid
glycyrrhizin
heparin
Inhibitor
methanol
MgCl2
-
2 mM, 24.8% inhibition
MnCl2
-
2 mM, 31.9% inhibition
NaCl
-
between 0.075 and 0.25 M, the activity is decreased with increasing NaCl concentration. Above 0.25 M, the enzyme shows no activity. The activity of beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide is decreased with increasing NaCl concentrations, and 0.4 M NaCl produces 50% inhibition
NEM
-
IC50: 0.09 mM
NiCl2
-
2 mM, 52% inhibition
p-chloromercuribenzoate
-
-
p-Chloromercuriphenylsulfonic acid
-
IC50: 0.04 mM
p-nitrophenol
-
IC50: 0.3 mM, competitive
paracetamol glucuronide
-
-
Phenolic and alcoholic glucuronides
-
-
-
Potassium saccharate
-
-
S-saccharic acid 1,4-lactone
-
inhibits enzyme in several patients with colon cancer in addition to the healthy controls
saccharic acid 1,4-lactone
saccharo-1,4-lactone
silybin
-
IC50: 1 mM, non-competitive
Sodium desoxycholate
-
-
TLCK
-
IC50: 0.3 mM
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
abscisic acid
-
1.49fold increased enzyme activity after application of 100 micromo/l abscisic acid for 6 h
azide
-
50 mM-0.5 M, stimulation of mutant enzyme E451A
bovine serum albumin
chlorpyrifos-methyl
-
-
diazinon
-
-
dichlorvos
-
-
ethyleneglycol
-
activation
fenitrothion
-
-
fenthion
-
-
lactose
-
uncompetitive activator
pectin-protein complex
-
adsorption of beta-glucuronidase on biopolymers is studied by the retention of the enzyme on the membrane of a concentrator with a pore diameter of 300 kDa and by native PAGE. Pectin-protein complexes (fractions PPC/CP and PPC/C) are established to increase the activity of beta-glucuronidase by 50 and 100%, respectively. There is a positive correlation between the increase of beta-glucuronidase activity in the presence of carbohydrates and enzyme adsorption on the polymers. The activity of the enzyme in the gel after electrophoresis of the PPC/+ beta-glucuronidase mixture is inversely proportional to the concentration of PPC/C in the mixture
-
Plasma serum
-
acivation
-
propetamphos
-
-
salicylic acid
-
44.6% increase of enzyme activity after treatment with 1 mmol/l salicylic acid for 5 h
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.49
1-Deoxy-1-(6-thiopurinyl)-beta-D-glucopyranoside
-
-
9.25
1-Deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosiduronamide
-
-
1.4
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-beta-Gal-(1-3)-Gal
-
pH 4.6, 37C
1.9
4-Me-beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal
-
pH 4.6, 37C
3.6
4-Me-beta-GlcA-(1-6)-Gal
-
pH 4.6, 37C
0.54 - 0.58
4-methylumbelliferyl beta-D-glucuronide
0.041 - 2.76
4-methylumbelliferyl-beta-D-glucuronide
3.06
4-nitrophenyl beta-D-galactosiduronide
pH 6.5, 75C, recombinant His6-tagged enzyme
0.0304 - 145
4-nitrophenyl beta-D-glucoside
0.152 - 167.5
4-nitrophenyl beta-D-glucuronide
0.2
4-nitrophenyl beta-D-xylopyranoside
-
wild-type enzyme
0.208 - 1.4
4-nitrophenyl-beta-D-glucuronide
0.132
Ammonium 1-deoxy-1-(6-thiopurinyl)-beta-D-glucopyranosidurate
-
-
0.0097 - 0.027
baicalein 7-O-beta-D-glucuronide
0.01 - 0.038
Baicalin
0.53
beta-GlcA-(1-6)-beta-Gal-(1-6)-Gal
-
pH 4.6, 37C
0.71
beta-GlcA-(1-6)-Gal
-
pH 4.6, 37C
0.91
carboxyumbelliferyl-beta-D-glucuronide
-
pH 6.5, 38C, Vmax: 0.041 mM/min
6.3
Chondroitin
-
pH 5.0, 37C
0.272
daidzein 7-O-beta-D-glucuronide
-
pH 7.2, 37C
0.266
equol 7-O-beta-D-glucuronide
-
pH 7.2, 37C
0.2
estriol-3-glucuronide
-
estriol-16-alpha-glucuronide
0.378 - 0.422
GlcAbeta(1-6)Gal
1.72 - 7.72
glycyrrhizin
0.025
kaempferol 3-O-beta-D-glucuronide
-
pH 7.2, 37C
0.009
luteolin 3'-O-beta-glucuronide
-
28C, pH 6.5
0.007
luteolin 7-O-diglucuronide 4'-O-glucuronide
-
-
0.065
methylumbelliferyl-beta-D-glucuronide
-
pH 6.5, 38C, Vmax: 0.026 mM/min
0.02
oestrone-3-glucuronide
-
-
0.04
oroxylin-7-O-beta-D-glucuronide
-
28C, pH 6.5
0.13 - 2.9
p-nitrophenyl-beta-D-glucuronide
0.018 - 3.08
phenolphthalein glucuronide
2.907
phenolphthalein-beta-D-glucuronic acid
-
pH 5.0, 52C
0.048
quercetin 4'-O-beta-D-glucuronide
-
pH 7.2, 37C
0.237
quercetin 7-O-beta-D-glucuronide
-
pH 7.2, 37C
0.167
quercetin-3-O-beta-D-glucuronide
-
pH 7.2, 37C
0.03
wogonin 7-O-beta-D-glucuronide
-
28C, pH 6.5
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 276
4-methylumbelliferyl-beta-D-glucuronide
443
4-nitrophenyl beta-D-galactosiduronide
Thermotoga maritima
Q9X0F2
pH 6.5, 75C, recombinant His6-tagged enzyme
9
4-nitrophenyl beta-D-glucoside
Thermotoga maritima
Q9X0F2
pH 6.5, 75C, recombinant His6-tagged enzyme
0.001 - 68
4-nitrophenyl beta-D-glucuronide
68
4-nitrophenyl beta-D-xylopyranoside
Escherichia coli
-
wild-type enzyme
0.67 - 1500
4-nitrophenyl-beta-D-glucuronide
200
4-nitrophenyl-beta-glucuronide
Mus musculus
-
-
0.011 - 639
baicalein 7-O-beta-D-glucuronide
1.07
daidzein 7-O-beta-D-glucuronide
Homo sapiens
-
pH 7.2, 37C
0.35
equol 7-O-beta-D-glucuronide
Homo sapiens
-
pH 7.2, 37C
23.3 - 27.84
glycyrrhizin
0.65
kaempferol 3-O-beta-D-glucuronide
Homo sapiens
-
pH 7.2, 37C
0.26
quercetin 4'-O-beta-D-glucuronide
Homo sapiens
-
pH 7.2, 37C
1.65
quercetin 7-O-beta-D-glucuronide
Homo sapiens
-
pH 7.2, 37C
1.9
quercetin-3-O-beta-D-glucuronide
Homo sapiens
-
pH 7.2, 37C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.61 - 13.55
glycyrrhizin
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.024
D-saccharic acid 1,4-lactone
pH 6.5, 75C, recombinant His6-tagged enzyme
0.9 - 1.2
estradiol 3-glucuronide
0.47 - 0.57
glycyrrhizin
1.6 - 2
paracetamol glucuronide
additional information
additional information
-
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0009
1,1'-(3-methyl-4-phenylthieno[2,3-b]thiophene-2,5-diyl)bis(4,4,4-triethoxybut-2-en-1-one)
Homo sapiens
-
pH not specified in the publication, 37C
-
7.5
D-glucuronate
Escherichia coli
-
IC50: 7.5 mM, competitive
0.0458
D-saccharic acid 1,4-lactone
Homo sapiens
-
pH not specified in the publication, 37C
2.32
fucoxanthin
Escherichia coli
-
pH not specified in the publication, at 37C
-
0.08
glycyrrhizin
Escherichia coli
-
IC50: 0.08 mM, competitive
0.09
NEM
Escherichia coli
-
IC50: 0.09 mM
0.04
p-Chloromercuriphenylsulfonic acid
Escherichia coli
-
IC50: 0.04 mM
0.3
p-nitrophenol
Escherichia coli
-
IC50: 0.3 mM, competitive
0.09
saccharic acid 1,4-lactone
Escherichia coli
-
IC50: 0.09 mM, competitive
1
silybin
Escherichia coli
-
IC50: 1 mM, non-competitive
0.3
TLCK
Escherichia coli
-
IC50: 0.3 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000144
-
at pH 7.0 and 37C
0.08
activity in crude cell extract from cell after growth for 9 h, activity-growth curve
0.127
-
beta-glucuronidase acting on chondroitin
0.137
-
purified isozyme I
0.19
-
purified isozyme II
9.37
-
purified enzyme, substrate 4-nitrophenyl beta-D-glucuronide
22.6
-
reaction with p-nitrophenyl-beta-D-glucuronide
23
-
pH 5.0, 45C
30.6
-
pH 4.0, 37C
31.33
-
-
100 - 533
-
-
117
-
purified recombinant enzyme, at pH 7.0 and 37C
160
-
purified recombinant enzyme, at pH 5.6 and 37C
173.4
-
activity with wogonin 7-O-beta-D-glucuronide
270
-
purified recombinant enzyme, at pH 7.0 and 52C
1284
-
purified recombinant enzyme
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.6
-
form III
3.2
-
form II
4 - 4.5
-
-
4 - 5
-
mutant enzyme E451A
4 - 4.5
-
-
4 - 4.4
-
acetate buffer
4.1
-
form II
4.5 - 6.5
highly active in the pH-range 4.5-6.5
5.4
-
citrate buffer
7.6
-
assay at
8
-
assay at, recombinant RFP-tagged enzyme
additional information
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.1 - 5.8
-
half maximal activity
3 - 6.5
-
pH profile, liver enzyme
3 - 7
-
pH profile, kidney enzyme
3 - 5
-
-
4 - 9
-
pH profile
4 - 7
pH 4.0: about 50% of maximal activity, pH 7.0: 25% of maximal activity
4 - 5.5
4
-
70% activity
5 - 8.5
-
pH 5.0: about 45% of maximal activity, pH 8.5: about 50% of maximal activity
6 - 7
activity is limited in neutral pH ranges of 6-7
6
-
90% activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56
-
-
additional information
-
assay carried out at room temperature
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 70
-
temperature profile
37 - 75
37C: about 60% of maximal activity, 75C: about 90% of maximal activity
37 - 100
37C: about 40% of maximal activity, 100C: about 50% of maximal activity
38 - 70
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
isoelectric focusing, pH-range: 3.5-10
5.1
-
calculated
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
Pectinex Ultra SP-L, Lot No. G 770
Manually annotated by BRENDA team
-
the enzyme is present during embryogenesis
Manually annotated by BRENDA team
-
the enzyme is present during embryogenesis
Manually annotated by BRENDA team
-
of patients with colon cancer and healthy controls
Manually annotated by BRENDA team
-
expresses only the lysosomal form of the enzyme
Manually annotated by BRENDA team
-
activity in round spermatids is detected in multivesicular bodies and acrosomal vesicles, activity in elongated spermatids is detected in sickle-shaped acrosome and in the residual body
Manually annotated by BRENDA team
-
activity in lysosome-like bodies
Manually annotated by BRENDA team
-
activity in acrosome and in cytoplasmic droplets
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
activity in acrosome and in cytoplasmic droplets. Activity in round spermatids is detected in acrosomal vesicles, activity in elongated spermatids is detected in sickle-shaped acrosome
-
Manually annotated by BRENDA team
-
the beta-glucuronidase forms a complex with esterase 22 in the liver. Gus colocalizes with Es22 at the endoplasmic reticulum but does not affect its RE hydrolase activity
Manually annotated by BRENDA team
-
recombinant enzyme in BHK cells
Manually annotated by BRENDA team
-
form I and II
Manually annotated by BRENDA team
additional information
-
subcellular distribution
-
Manually annotated by BRENDA team
PDB
SCOP
CATH
ORGANISM
UNIPROT
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Acidobacterium capsulatum (strain ATCC 51196 / DSM 11244 / JCM 7670 / NBRC 15755 / NCIMB 13165 / 161)
Clostridium perfringens (strain 13 / Type A)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Escherichia coli (strain K12)
Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
Streptococcus agalactiae serotype V (strain ATCC BAA-611 / 2603 V/R)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
gel filtration
70000
-
gel filtration, SDS-PAGE
71000
-
gel filtration
113500
-
sedimentation equilibrium centrifugation of form I
130000
-
gel filtration, beta-glucuronidase acting on chondroitin
140000
-
isozymes I and II, gel filtration
160000
-
SDS-PAGE
180000
-
-
200000 - 220000
-
gel filtration
230000
-
gel filtration
250000 - 310000
280000 - 300000
-
gel filtration, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide
290000
-
gel filtration
300000
-
SDS-PAGE
390000
-
wild-type enzyme, gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homotetramer
-
4 * 75000, SDS-PAGE
monomer
tetramer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
phosphoprotein
-
both the placental 80000 Da and 64000 Da peptides, though not the 18000 Da one, are apparently phosphorylated, whereas the enzyme of leukemic cells is poorly phosphorylated
proteolytic modification
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
3D model shows catalytic resiudes E396, E508, and Y471in loop regions
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1 - 7
-
stable
697118
2 - 9
-
4C, 24 h, stable
655172
3 - 10.5
-
-
26871
4 - 11
-
-
26867
4.2 - 7
-
form III
26847
4.5 - 9
-
37C, 3 h, stable
655172
5 - 7
-
form I
26847
5 - 8
-
80 min, purified recombinant enzyme, 37C, completely stable
709499
5
-
inactivation
26863
5.4 - 7
-
form II
26847
6 - 9
stable
697118
6.5
-
inactivation
26863
7
-
or 7.8, stable at, depending on buffer
26868
8
-
stable at
26863
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
-20
-
inactivation
71
-
30 min, pH 5.0, 80% activity, 30 min, pH 8.0, 20% activity
75
-
pH 4.6, 20 min, complete inactivation
85
recombinant His6-tagged enzyme, half-life is 3 h
GENERAL STABILITY