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3.2.1.147: thioglucosidase

This is an abbreviated version!
For detailed information about thioglucosidase, go to the full flat file.

Word Map on EC 3.2.1.147

Reaction

A thioglucoside
+
H2O
=
a sugar
 +
a thiol

Synonyms

ArMy2, AtBGLU37, AtBGLU38, atypical myrosinase, beta-glucosidase 26, peroxisomal, beta-glucosidase 37, beta-glucosidase 38, beta-thioglucosidase, beta-thioglucosidase glucohydrolase, beta-thioglucoside glucohydrolase, beta-thioglucoside glucohydrolase,, BGLU26, CpTGG1, CpTGG2, EC 3.2.3.1, glucosidase, thio-, glucosinolase, More, MYR, MYR II, MYR1 myrosinase, Myr1.Bn1, Myr2.Bn1, MYRc, MYRI, MYRII, myrosin, myrosinase, myrosinase 1, myrosinase 2, myrosinase A, myrosinase B, PEN2, PYK10 myrosinase, sinigrase, sinigrinase, sinigrinase 1, sinigrinase 2, TGG, TGG1, TGG2, TGG4, TGG5, Thioglucosidase, thioglucosidase 1, thioglucosidase 2, thioglucoside glucohydrolase, thioglucoside glucohydrolase 1, thioglycosidase, WjMYR

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.147 thioglucosidase

Cloned

Cloned on EC 3.2.1.147 - thioglucosidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis
DNA and amino acid sequence determination and analysis, combining proteomics and transcriptomics analysis, phylogenetic analysis
expressed in Pichia pastoris
expression and secretion by Pichia pastoris
-
expression in Sf9 cell
expression of myrosinase MYR1 in Saccharomyces cerevisiae. The recombinant enzyme is enzymatically active which shows that the enzyme, which in plant is complex bound with myrosinase-binding proteins and myrosinase-associated proteins, is functional in its free form. Expression of the enzyme is transient and the growth of the yeast cells is significantly reduced during the period of expression of myrosinase
-
gene pTGG2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Pichia pastoris
gene tgg1, exxpression of recombinant TGG1, sequence comparisons
gene tgg2, recombinant expression of TGG2, sequence comparisons
myrosinase is encoded by two different families of genes: MA and MB. The MA type myrosinases are encoded by approximately 4 genes, the MB type myrosinases are encoded by more than 10 genes
-
myrosinases are encoded by a multigene family consisting of two subgroups. Cloning and sequencing of the two nuclear genes, Myr1.Mn1 and Myr2.Mn1, representing each of these two subgroups
-
overexpression of His-tagged root isozymes TGG4 and TGG5 and His-tagged leaf isozyme TGG1 in Pichia pastoris
-
overexpression of nitrile-specifier protein 2, NSP2, in transgenic Arabidopsis thaliana plants leading to increased nitrile production
-
phylogenetic analysis and tree, recombinant expression of wild-type and mutant enzymes
-
phylogenetic tree, recombinant overexpression of His6-tagged ArMy2 in Pichia pastoris
recombinant gene Myr1.Bn1, expressionin transgenic Brassica napus plants