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3.2.1.147: thioglucosidase

This is an abbreviated version!
For detailed information about thioglucosidase, go to the full flat file.

Word Map on EC 3.2.1.147

Reaction

A thioglucoside
+
H2O
=
a sugar
+
a thiol

Synonyms

ArMy2, AtBGLU37, AtBGLU38, atypical myrosinase, beta-glucosidase 26, peroxisomal, beta-glucosidase 37, beta-glucosidase 38, beta-thioglucosidase, beta-thioglucosidase glucohydrolase, beta-thioglucoside glucohydrolase, beta-thioglucoside glucohydrolase,, BGLU26, CpTGG1, CpTGG2, EC 3.2.3.1, glucosidase, thio-, glucosinolase, More, MYR, MYR II, MYR1 myrosinase, Myr1.Bn1, Myr2.Bn1, MYRc, MYRI, MYRII, myrosin, myrosinase, myrosinase 1, myrosinase 2, myrosinase A, myrosinase B, PEN2, PYK10 myrosinase, sinigrase, sinigrinase, sinigrinase 1, sinigrinase 2, TGG, TGG1, TGG2, TGG4, TGG5, Thioglucosidase, thioglucosidase 1, thioglucosidase 2, thioglucoside glucohydrolase, thioglucoside glucohydrolase 1, thioglycosidase, WjMYR

ECTree

     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.147 thioglucosidase

Purification

Purification on EC 3.2.1.147 - thioglucosidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes
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at least 2 isoenzymes
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complexes with myrosinase and myrosinase-binding protein
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Con-A-Sepharose column chromatography, SP-Sepharose column chromatography, and Superdex 200 gel filtration
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fast and gentle procedure for hte isolation of enzyme complex from seed
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fast and gentle procedure for the isolation of enzyme complex from seed
immobilized metal affinity chromatography
myrosinase A, B and C
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myrosinase C1, C2 and C3
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myrosinase I and II
-
native enzyme 1318fold from broccoli by ammonium sulfate fractionation followed by concanavalin A affinity chromatography, with an intermediate dialysis step
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native enzyme 69.3fold from leaves to homogeneity by ammonium sulfate fractionation, ultrafiltration, concanavalin A affinity chromatography, and gel filtation
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native enzyme by three chromatographic step involving gel filtration
native enzyme from cell-free seed extract by ammonium sulfate fractionation
-
native enzyme from freeze-dried 3-day-old broccoli sprouts 5.51fold for mobile enzyme fraction, and 14.6fold from counter-current chromatography
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native enzyme from freeze-dried 7-day-old daikon sprouts 3.45fold for mobile enzyme fraction, and 22.9fold from counter-current chromatography
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native enzyme from freeze-dried leaves 2.20fold for mobile enzyme fraction, and 61.5fold from counter-current chromatography
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native enzyme from inflorescences by ammonium sulfate fractionation, dialysis, concanavalin A affinity chromatography, and gel filtration
native enzyme from leaves
-
native enzyme from powder of freeze-dried seedlings of Brussels sprouts, by solubilization, and concanavalin A affinity chromatography with elution by methyl-alpha-D-mannopyranoside
native enzyme from root by ammonium sulfate fractionation and gel filtration
-
native enzyme from roots
native enzyme from seedlings or roots
native enzyme from seedlings or seeds
-
native enzyme from seedlings, partially from seed
native enzyme from seeds
native enzyme from seeds 4.54fold for mobile enzyme fraction, and 41.8fold from counter-current chromatography
-
native enzyme from sprouts
native enzyme partially
-
native isozyme CpTGG1 from leaves
native isozyme CpTGG2 from roots
native isozyme TGG1 from leaves
native isozyme TGG2 from leaves
native isozyme TGG4 from roots
native isozyme TGG5 from roots
partial purification of the seed enzyme
rapid isolation of catalytically active plant myrosinase from 3-day-old sprouts at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation
rapid isolation of catalytically active plant myrosinase from 7-day-old sprouts at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation
rapid isolation of catalytically active plant myrosinase from fresh leaves at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation
-
rapid isolation of catalytically active plant myrosinase from fresh seed powder at high yield and purity, involving three main steps: (i) selective solvation, (ii) counter-current chromatography (CCC) using an aqueous two-phase system (ATPS) mixture of potassium phosphate and polyethylene glycol (PEG), and (iii) in-line desalting. This is followed by ultrafiltration and concanavalin A affinity chromatography. Method optimization and evaluation
-
recombinant
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recombinant His-tagged root isozymes TGG4 and TGG5 and His-tagged leaf isozyme TGG1 from Pichia pastoris by nickel affinity chromatography to homogeneity
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recombinant His6-tagged ArMy2 from Pichia pastoris by nickel affinity chromatography
recombinant TGG2 from Pichia pastoris
TGG1 51.9fold from from T-DNA insertion lines of Arabidopsis using lectin affinity and anion exchange chromatography
TGG2 84.2fold from from T-DNA insertion lines of Arabidopsis using lectin affinity and anion exchange chromatography
three different forms of enzyme: Ca, Cb and Cc
-