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(2R)-2-hydroxy-2-phenylethylglucosinolate + H2O
D-glucose + ?
(2S)-2-hydroxy-3-butenyl glucosinolate + H2O
D-glucose +
-
i.e. epi-progoitrin
presence of endogenous epithiospecifier protein directs the reaction toward formation of an epithionitrile
-
?
(R)-4-methylsulfinylbutyl glucosinolate + H2O
?
-
i.e. glucoraphanin or GRP
-
-
?
1-methoxyindol-3-ylmethyl-glucosinolate + H2O
1-methoxyindol-3-ylmethyl isothiocyanate + D-glucose
2-(4-hydroxyphenyl)ethylglucosinolate + H2O
D-glucose + 4-(2-carboxy-1-hydroxyethyl)phenyl sulfate
-
-
-
-
?
2-(4-methoxyphenyl)ethylglucosinolate + H2O
D-glucose + 4-(2-cyano-1-hydroxyethyl)phenyl sulfate + 4-(2-cyanoethyl)phenyl sulfate
-
-
-
-
?
2-hydroxy-3-butenylglucosinolate + H2O
5-vinyl-2-oxazolidine thione + D-glucose
-
-
-
-
?
2-hydroxy-3-butenylglucosinolate + H2O
?
-
-
-
?
2-hydroxybut-3-enylglucosinolate + H2O
?
-
-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
-
-
-
?
2-phenylethylglucosinolate + H2O
2-phenylethyl-isothiocyanate + D-glucose
-
-
-
-
?
2-phenylethylglucosinolate + H2O
?
-
-
-
?
2-propenylglucosinolate + H2O
2-propenyl-isothiocyanate + D-glucose
-
epithiospecifier protein and nitrile-specifier protein can switch myrosinase-catalyzed degradation of 2-propenylglucosinolate from isothiocyanate to nitrile, only epithiospecifier protein generates the corresponding epithionitrile
-
-
?
3-benzyloxypropylglucosinolate + H2O
3-benzyloxypropyl-isothiocyanate + D-glucose
-
-
-
-
?
3-butenylglucosinolate + H2O
3-butenyl-isothiocyanate + D-glucose
-
-
-
-
?
3-butenylglucosinolate + H2O
?
-
-
-
?
3-deoxyglucotropaeolin + H2O
?
-
-
-
-
?
3-methylthiopropylglucosinolate + H2O
3-methylthiopropyl-isothiocyanate + D-glucose
-
-
-
-
?
4-(methylsulfinyl)butyl glucosinolate + H2O
D-glucose + ?
-
i.e. glucoraphanin
presence of endogenous epithiospecifier protein directs the reaction toward formation of sulforaphane nitrile in place of the anticancerogenic sulforaphane
-
?
4-benzyloxybutylglucosinolate + H2O
4-benzyloxybutyl-isothiocyanate + D-glucose
-
-
-
-
?
4-deoxyglucotropaeolin + H2O
?
-
-
-
-
?
4-hydroxybenzyl glucosinolate + H2O
4-hydroxybenzyl isothiocyanate + D-glucose
-
-
-
-
?
4-hydroxybenzylglucosinolate + H2O
D-glucose + 4-hydroxyphenylacetamide sulfate + ?
-
-
-
-
?
4-hydroxybenzylglucosinolate + H2O
D-glucose + 4-hydroxyphenylacetonitrile sulfate + ?
-
-
-
-
?
4-methoxyindol-3-ylmethyl-glucosinolate + H2O
4-methoxyindol-3-ylmethyl isothiocyanate + D-glucose
4-methylsulfinylbutyl glucosinolate + H2O
4-methylsulfinylbutyl isothiocyanate + D-glucose
-
isolated from seeds of Sinapis alba
-
-
?
4-methylsulfinylbutylglucosinolate + H2O
4-methylsulfinylbutyl-isothiocyanate + D-glucose
-
-
-
-
?
4-methylsulfinylbutylglucosinolate + H2O
?
4-methylthiobutylglucosinolate + H2O
4-methylthiobutyl-isothiocyanate + D-glucose
-
-
-
-
?
4-methylthiobutylglucosinolate + H2O
?
4-methylumbelliferyl-beta-D-glucoside + H2O
4-methylumbelliferone + D-glucose
-
-
-
-
?
4-nitrophenyl 2-acetamido-2-deoxy-1-thio-beta-D-glucopyranoside + H2O
4-nitrobenzenethiol + N-acetyl-D-glucosamine
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
4-pentenylglucosinolate + H2O
4-pentenyl-isothiocyanate + D-glucose
-
-
-
-
?
4-pentenylglucosinolate + H2O
?
-
-
-
?
5-methylthiopentylglucosinolate + H2O
5-methylthiopentyl-isothiocyanate + D-glucose
-
-
-
-
?
6-deoxyglucotropaeolin + H2O
?
-
-
-
-
?
6-methylthiohexylglucosinolate + H2O
6-methylthiohexyl-isothiocyanate + D-glucose
-
-
-
-
?
7-methylthioheptylglucosinolate + H2O
7-methylthioheptyl-isothiocyanate + D-glucose
-
-
-
-
?
8-methylthiooctylglucosinolate + H2O
8-methylthiooctyl-isothiocyanate + D-glucose
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
allyl glucosinolate + H2O
?
-
-
-
?
allylglucosinolate + H2O
?
-
-
in presence of epithionitrile from Arabidopsis thaliana, formation of epithionitrile and nitrile. In presence of nitrile specifier protein from Pieris rapa, formation of nitrile
-
?
benzylglucosinolate + H2O
?
benzylglucosinolate + H2O
benzylisothiocyanate + D-glucose + ?
-
nitrile-specifier proteins, especially nitrile-specifier protein 2, NSP2, in conjunction with myrosinase enable the enzyme to generate nitriles, overview
-
-
?
benzylglucosinolate + H2O
D-glucose + hippuric acid + ?
epigoitrin + H2O
?
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
gluconapin + H2O
(1E)-N-(sulfooxy)pent-4-enimidothioic acid + D-glucose
gluconasturtiin + H2O
?
-
-
-
?
gluconasturtiin + H2O
D-glucose + ?
glucoraphanin + H2O
sulforaphane + D-glucose
glucoraphenin + H2O
?
-
3.8% of the activity with epi-progoitrin
-
-
?
glucosinolate + H2O
isothiocyanate + thiocyanate + nitrile + epithionitrile + ?
-
-
-
-
?
glucotropaeolin + H2O
D-glucose + ?
indol-3-ylmethyl glucosinolate + H2O
?
-
-
-
-
?
indol-3-ylmethyl glucosinolate + H2O
indo-3-ylmethyl isothiocyanate + D-glucose
-
high activity
-
-
?
indol-3-ylmethyl glucosinolate + H2O
indol-3-ylmethyl isothiocyanate + D-glucose
indolyl-3-methylglucosinolate + H2O
indolyl-3-methyl-isothiocyanate + D-glucose
-
-
-
-
?
nasturtin + H2O
?
-
best substrate, i.e. 2-phenylethyl glucosinolate
-
-
?
neo-glucobrassicin + H2O
?
-
-
-
-
?
p-hydroxybenzylglucosinolate + H2O
?
-
-
-
-
?
p-hydroxybenzylglucosinolate + H2O
D-glucose + ?
-
-
-
-
?
p-nitrophenyl beta-D-glucopyranoside + H2O
?
p-nitrophenyl beta-D-glucopyranoside + H2O
p-nitrophenol + D-glucose
-
-
-
-
?
phenylethylglucosinolate + H2O
?
-
-
-
-
?
progoitrin + H2O
(1E,3S)-3-hydroxy-n-(sulfooxy)pent-4-enimidothioic acid + D-glucose
-
-
-
-
?
scopolin + H2O
?
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
sinigrin + H2O
allyl isothiocyanate + D-glucose + ?
sinigrin + H2O
D-glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
sinigrin + H2O
D-glucose + isothiocyanate + ?
sinigrin + H2O
glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
2-hydroxybut-3-enylglucosinolate + H2O
additional information
-
(2R)-2-hydroxy-2-phenylethylglucosinolate + H2O
D-glucose + ?
-
i.e. glucosibarin
-
-
?
(2R)-2-hydroxy-2-phenylethylglucosinolate + H2O
D-glucose + ?
-
i.e. glucosibarin
-
-
?
1-methoxyindol-3-ylmethyl-glucosinolate + H2O
1-methoxyindol-3-ylmethyl isothiocyanate + D-glucose
-
-
-
?
1-methoxyindol-3-ylmethyl-glucosinolate + H2O
1-methoxyindol-3-ylmethyl isothiocyanate + D-glucose
-
-
-
?
4-methoxyindol-3-ylmethyl-glucosinolate + H2O
4-methoxyindol-3-ylmethyl isothiocyanate + D-glucose
-
-
-
?
4-methoxyindol-3-ylmethyl-glucosinolate + H2O
4-methoxyindol-3-ylmethyl isothiocyanate + D-glucose
-
-
-
?
4-methylsulfinylbutylglucosinolate + H2O
?
-
-
-
?
4-methylsulfinylbutylglucosinolate + H2O
?
-
-
-
?
4-methylsulfinylbutylglucosinolate + H2O
?
-
-
in presence of epithionitrile from Arabidopsis thaliana, formation of epithionitrile and nitrile. In presence of nitrile specifier protein from Pieris rapa, formation of nitrile
-
?
4-methylthiobutylglucosinolate + H2O
?
-
-
-
?
4-methylthiobutylglucosinolate + H2O
?
-
-
in presence of epithionitrile from Arabidopsis thaliana, formation of epithionitrile and nitrile. In presence of nitrile specifier protein from Pieris rapa, formation of nitrile
-
?
4-nitrophenyl 2-acetamido-2-deoxy-1-thio-beta-D-glucopyranoside + H2O
4-nitrobenzenethiol + N-acetyl-D-glucosamine
-
-
-
-
?
4-nitrophenyl 2-acetamido-2-deoxy-1-thio-beta-D-glucopyranoside + H2O
4-nitrobenzenethiol + N-acetyl-D-glucosamine
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
a poor substrate for ArMy2
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
a thioglucoside + H2O
a sugar + a thiol
-
-
-
-
?
benzylglucosinolate + H2O
?
-
-
-
?
benzylglucosinolate + H2O
?
-
-
in presence of epithionitrile from Arabidopsis thaliana, formation of epithionitrile and nitrile. In presence of nitrile specifier protein from Pieris rapa, formation of nitrile
-
?
benzylglucosinolate + H2O
D-glucose + hippuric acid + ?
-
-
-
-
?
benzylglucosinolate + H2O
D-glucose + hippuric acid + ?
-
-
-
-
?
epi-progoitrin + H2O
?
-
-
-
-
?
epi-progoitrin + H2O
?
-
i.e. 2(S)-2-hydroxy-3-butenyl glucosinolate
-
-
?
glucoapparin + H2O
?
-
-
-
-
?
glucoapparin + H2O
?
-
-
-
-
?
glucobrassicin + H2O
?
-
-
-
?
glucobrassicin + H2O
?
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucobrassicin + H2O
indol-3-ylmethylisothiocyanate + D-glucose
-
-
-
-
?
glucocheirolin + H2O
?
-
-
-
-
?
glucocheirolin + H2O
?
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucocochlearin + H2O
(1E)-2-methyl-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
glucoiberin + H2O
(1E)-4-(methylsulfinyl)-N-(sulfooxy)butanimidothioic acid + D-glucose
-
-
-
-
?
gluconapin + H2O
(1E)-N-(sulfooxy)pent-4-enimidothioic acid + D-glucose
-
-
-
-
?
gluconapin + H2O
(1E)-N-(sulfooxy)pent-4-enimidothioic acid + D-glucose
-
-
-
-
?
gluconapin + H2O
(1E)-N-(sulfooxy)pent-4-enimidothioic acid + D-glucose
-
-
-
-
?
gluconapin + H2O
(1E)-N-(sulfooxy)pent-4-enimidothioic acid + D-glucose
-
-
-
-
?
gluconasturtiin + H2O
D-glucose + ?
-
-
-
-
?
gluconasturtiin + H2O
D-glucose + ?
-
-
-
-
?
glucoraphanin + H2O
sulforaphane + D-glucose
-
-
-
?
glucoraphanin + H2O
sulforaphane + D-glucose
-
-
-
-
?
glucosinalbin + H2O
?
-
-
-
-
?
glucosinalbin + H2O
?
-
9.5% of the activity with epi-progoitrin
-
-
?
glucosinalbin + H2O
?
-
-
-
-
?
glucosinalbin + H2O
?
-
-
-
-
?
glucotropaeolin + H2O
?
-
-
-
-
?
glucotropaeolin + H2O
?
-
-
-
-
?
glucotropaeolin + H2O
?
-
-
-
?
glucotropaeolin + H2O
?
-
7.8% of the activity with epi-progoitrin
-
-
?
glucotropaeolin + H2O
?
-
-
-
-
?
glucotropaeolin + H2O
?
-
-
-
-
?
glucotropaeolin + H2O
D-glucose + ?
-
-
-
-
?
glucotropaeolin + H2O
D-glucose + ?
-
-
-
-
?
indol-3-ylmethyl glucosinolate + H2O
indol-3-ylmethyl isothiocyanate + D-glucose
-
-
-
?
indol-3-ylmethyl glucosinolate + H2O
indol-3-ylmethyl isothiocyanate + D-glucose
-
-
-
?
p-nitrophenyl beta-D-glucopyranoside + H2O
?
-
-
-
-
?
p-nitrophenyl beta-D-glucopyranoside + H2O
?
-
-
-
-
?
p-nitrophenyl beta-D-glucopyranoside + H2O
?
-
-
-
-
?
p-nitrophenyl beta-D-glucopyranoside + H2O
?
-
-
-
-
?
progoitrin + H2O
?
-
-
-
-
?
progoitrin + H2O
?
-
9.8% of the activity with epi-progoitrin
-
-
?
sinalbin + H2O
?
-
-
-
-
?
sinalbin + H2O
?
-
i.e. 4-hydroxybenzyl glucosinolate
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
best substrate
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
(1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
-
-
-
?
sinigrin + H2O
?
-
-
-
?
sinigrin + H2O
?
-
-
-
-
?
sinigrin + H2O
?
-
-
-
-
?
sinigrin + H2O
?
-
-
-
-
?
sinigrin + H2O
allyl isothiocyanate + D-glucose + ?
-
i.e. 2-propenyl glucosinolate
-
-
?
sinigrin + H2O
allyl isothiocyanate + D-glucose + ?
i.e. 2-propenyl glucosinolate
-
-
?
sinigrin + H2O
allyl isothiocyanate + D-glucose + ?
i.e. 2-propenyl glucosinolate
-
-
?
sinigrin + H2O
allyl isothiocyanate + D-glucose + ?
i.e. 2-propenyl glucosinolate, substrate saturation at 6 mM
-
-
?
sinigrin + H2O
allyl isothiocyanate + D-glucose + ?
-
i.e. 2-propenyl glucosinolate
-
-
?
sinigrin + H2O
D-glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
-
-
-
?
sinigrin + H2O
D-glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
15.3% of the activity with epi-progoitrin
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
Brassica caulorapa
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
208545, 208548, 208550, 208557, 208563, 208565, 208566, 208571, 208578, 208580, 208583, 208584, 208585, 208586, 208589 -
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
2-propenylglucosinolate
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
Paracolobactrum aerogenoides
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
208544, 208548, 208549, 208551, 208552, 208553, 208554, 208557, 208563, 208565, 208571, 208572, 208579, 208580, 208590, 208591, 208593 -
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
Sinapis sp.
-
-
-
-
?
sinigrin + H2O
D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
-
-
-
?
sinigrin + H2O
D-glucose + isothiocyanate + ?
-
-
-
-
?
sinigrin + H2O
D-glucose + isothiocyanate + ?
-
-
-
-
?
sinigrin + H2O
D-glucose + isothiocyanate + ?
-
i.e. allylglucosinolate
-
-
?
sinigrin + H2O
D-glucose + isothiocyanate + ?
-
-
-
?
sinigrin + H2O
D-glucose + isothiocyanate + ?
-
-
-
-
?
sinigrin + H2O
D-glucose + isothiocyanate + ?
-
-
-
-
?
sinigrin + H2O
D-glucose + isothiocyanate + ?
-
i.e. 2-propenyl glucosinolate
-
-
?
sinigrin + H2O
glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
-
-
-
?
sinigrin + H2O
glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
-
-
-
?
sinigrin + H2O
glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
-
-
-
?
sinigrin + H2O
glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
-
-
-
?
sinigrin + H2O
glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
-
-
-
?
sinigrin + H2O
glucose + (1Z)-N-(sulfooxy)but-3-enimidothioic acid
-
-
-
-
?
2-hydroxybut-3-enylglucosinolate + H2O
additional information
-
-
-
in absence of ascorbate 5-vinyloxazolidine-2-thione is the only product, whereas in the presence of added ascorbate 1-cyano-2-hydroxybut-3-ene is also formed
?
2-hydroxybut-3-enylglucosinolate + H2O
additional information
-
-
-
5-vinyloxazolidine-2-thione is the sole product above pH 5.4, reaching a maximum at pH 8.0, whilst 1-cyano-2-hydroxybut-3-ene is the main product at low pH reaching a maximum at pH 3.4
?
2-hydroxybut-3-enylglucosinolate + H2O
additional information
-
-
(S)-2-hydroxy-3-butenylglucosinolate, i.e. epi-progoitrin
a mixture of products which includes 1-cyano-2-hydroxy-3-butene, (R)-5-vinyloxazolidine-2-thione, D-glucose, HSO4- and elemental sulfur is formed without epithiospecifier protein at pH 5.9. These products as well as erythro-1-cyano-2-hydroxy-3,4-epithiobutanes and threo-1-cyano-2-hydroxy-3,4-epithiobutanes are formed by combination of the enzyme and epithiospecifier protein from various sources
?
2-phenethylglucosinolate + H2O
additional information
-
-
-
in presence of ascorbic acid, isothiocyanate and nitrile are obtained but no thiocyanate
?
2-phenethylglucosinolate + H2O
additional information
-
Sinapis sp.
-
-
nitrile formation is favoured at lower pH levels, the ratio of nitrile to isothiocyanate is directly related to the hydrogen ion concentration of the medium
?
allylglucosinolate + H2O
additional information
-
-
-
conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein
?
allylglucosinolate + H2O
additional information
-
-
-
in presence of epithiospecifier protein 1-cyano-2,3-epithiopropane and allyl isothiocyanate are formed, in absence of epithiospecifier protein only allyl isothiocyanate is formed
?
allylglucosinolate + H2O
additional information
-
-
-
-
-
?
allylglucosinolate + H2O
additional information
-
-
-
conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein
?
allylglucosinolate + H2O
additional information
-
-
-
conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein
?
allylglucosinolate + H2O
additional information
-
-
-
conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein
?
allylglucosinolate + H2O
additional information
-
-
-
in presence of ascorbic acid, isothiocyanate and nitrile are obtained but no thiocyanate
?
allylglucosinolate + H2O
additional information
-
-
-
conversion to 1-cyano-2,3-epithiopropane in presence of epithiospecifier protein, conversion to allyl cyanide in absence of epithiospecifier protein
?
allylglucosinolate + H2O
additional information
-
-
-
in presence of epithiospecifier protein 1-cyano-2,3-epithiopropane and allyl isothiocyanate are formed, in absence of epithiospecifier protein only allyl isothiocyanate is formed
?
allylglucosinolate + H2O
additional information
-
Sinapis sp.
-
-
nitrile formation is favoured at lower pH levels, the ratio of nitrile to isothiocyanate is directly related to the hydrogen ion concentration of the medium
?
benzylglucosinolate + H2O
additional information
-
-
-
-
-
?
benzylglucosinolate + H2O
additional information
-
-
-
-
?
additional information
?
-
substrate specificity, overview
-
-
?
additional information
?
-
substrate specificity, overview
-
-
?
additional information
?
-
-
substrate specificity, overview
-
-
?
additional information
?
-
-
the degradation of glucosinolates is catalyzed by thioglucosidases called myrosinases and leads by default to the formation of isothiocyanates
-
-
?
additional information
?
-
-
differences in basal activity of myrosinase isozymes, no activity with desulfosinigrin
-
-
?
additional information
?
-
-
myrosinase acts on glucosinolates to form an unstable aglycone intermediate that can rearrange spontaneously to form an isothiocyanate. Interaction of a protein called epithiospecifier protein with myrosinase diverts the reaction toward the production of epithionitriles or nitriles depending on the glucosinolate structure, while nitrile-specifier proteins, especially nitrile-specifier protein 2, NSP2, enable to generate nitriles in conjunction with myrosinase, tissue distributions of the specifier proteins, overview
-
-
?
additional information
?
-
myrosinases catalyze the hydrolysis of glucosinolates, a structurally distinct group of nitrogen- and sulfur-containing secondary metabolites
-
-
?
additional information
?
-
myrosinases catalyze the hydrolysis of glucosinolates, a structurally distinct group of nitrogen- and sulfur-containing secondary metabolites
-
-
?
additional information
?
-
-
myrosinases catalyze the hydrolysis of glucosinolates, a structurally distinct group of nitrogen- and sulfur-containing secondary metabolites
-
-
?
additional information
?
-
-
indole glucosinolates are in planta substrates for PYK10
-
-
?
additional information
?
-
-
enzyme PYK10 has in vitro myrosinase activity toward indole glucosinolates. PYK10 exhibits both O-glucosidase and S-glucosidase activity. No activity against sinigrin. PYK10 hydrolyzes both coumarin glucosides and glucosinolates, and accounts for the bulk of total myrosinase activity against indol-3-ylmethyl glucosinolate in Arabidopsis thaliana roots
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
the enzyme is active with indole and aliphatic glucosinolates
-
-
?
additional information
?
-
the enzyme is active with indole and aliphatic glucosinolates
-
-
?
additional information
?
-
the enzyme is active with indole and aliphatic glucosinolates
-
-
?
additional information
?
-
the enzyme is active with indole and aliphatic glucosinolates
-
-
?
additional information
?
-
myrosinase-catalyzed deglycosylation of a glucosinolate releases beta-D-glucose and an unstable intermediate, which undergoes rearrangements to finally yield an isothiocyanate
-
-
?
additional information
?
-
-
myrosinase-catalyzed deglycosylation of a glucosinolate releases beta-D-glucose and an unstable intermediate, which undergoes rearrangements to finally yield an isothiocyanate
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
hydrolysis of rapeseed meal yields 1-cyano-2-hydroxy-3-butene, 5-vinyloxazolidine-2-thione, 3-butenylisothiocyanate and 4-pentenylisothiocyanate
-
-
?
additional information
?
-
MYR is a beta-thioglucosidase that hydrolyses glucosinolates to a variety of products such as isothiocyanates, thiocyanates, nitriles, epithionitriles, and oxazolidine-thiones depending on the nature of the glucosinolates. Glucosinolates are themselves biologically inactive, but glucosinolates hydrolytic products, such as thiocyanates, isothiocyanates, nitriles, and oxazolidine-2-thione, produced by MYR during processing of oilseed rape meal are biologically active. Glucosinolate profiles of wild-type and mutant plants, overview
-
-
?
additional information
?
-
-
MYR is a beta-thioglucosidase that hydrolyses glucosinolates to a variety of products such as isothiocyanates, thiocyanates, nitriles, epithionitriles, and oxazolidine-thiones depending on the nature of the glucosinolates. Glucosinolates are themselves biologically inactive, but glucosinolates hydrolytic products, such as thiocyanates, isothiocyanates, nitriles, and oxazolidine-2-thione, produced by MYR during processing of oilseed rape meal are biologically active. Glucosinolate profiles of wild-type and mutant plants, overview
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates. Ioszyme MYRI is maximally active against aliphatic glucosinolate followed by aromatic glucosinolates, and indole glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates. Ioszyme MYRI is maximally active against aliphatic glucosinolate followed by aromatic glucosinolates, and indole glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates. Ioszyme MYRII is maximally active against aliphatic glucosinolate followed by aromatic glucosinolates, and indole glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates. Ioszyme MYRII is maximally active against aliphatic glucosinolate followed by aromatic glucosinolates, and indole glucosinolates
-
-
?
additional information
?
-
-
in intact plant tissues, the enzyme is physically separated from its GSL substrates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
substrate docking analysis. modeling of glucoraphanin and sinigrin into the regulatory and the active site, three-dimensional structure model of broccoli myrosinase, modeling and validation, overview
-
-
?
additional information
?
-
-
substrate docking analysis. modeling of glucoraphanin and sinigrin into the regulatory and the active site, three-dimensional structure model of broccoli myrosinase, modeling and validation, overview
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
recombinant TGG1 has weak ascorbic acid-independent O-beta-glucosidase activity with substrate specificity
-
-
?
additional information
?
-
-
recombinant TGG1 has weak ascorbic acid-independent O-beta-glucosidase activity with substrate specificity
-
-
?
additional information
?
-
the enzyme is inactive towards glucovanillin and n-octyl-beta-D-glucopyranoside
-
-
?
additional information
?
-
-
the enzyme is inactive towards glucovanillin and n-octyl-beta-D-glucopyranoside
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
myrosinase in general catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates. Isozyme TGG1 is an ascorbate independent O-beta-glucosidase activity
-
-
?
additional information
?
-
myrosinase in general catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates. Isozyme TGG1 is an ascorbate independent O-beta-glucosidase activity
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates. The enzyme from Crambe abyssinica is highly specific for epi-progoitrin
-
-
?
additional information
?
-
-
induced by addition of 0.01% sinigrin and 6% mustard extract
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
induced by addition of 0.01% sinigrin and 6% mustard extract
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
the enzyme produces nitriles from desulfoglucosinolates
-
-
?
additional information
?
-
-
the enzyme produces nitriles from desulfoglucosinolates
-
-
?
additional information
?
-
-
compositions and contents of glucosinolates in salt cress, Thellungiella halophila, at different developmental stages, HPLC-MS analysis, profiles, overview
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview. Paraphoma radicina strain E5 shows a low overall activity on glucosinolates compared to other endophytic fungi
-
-
?
additional information
?
-
-
no activity with sinigrin
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview. Paraphoma radicina strain E5 shows a low overall activity on glucosinolates compared to other endophytic fungi
-
-
?
additional information
?
-
-
no activity with sinigrin
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
-
-
?
additional information
?
-
-
decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview. Paraphoma radicina strain E5 shows a low overall activity on glucosinolates compared to other endophytic fungi
-
-
?
additional information
?
-
-
no activity with sinigrin
-
-
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additional information
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highest myrosinase activity is detected with allylglucosinolate. Activity with 3-butenylglucosinolate, 4-pentenylglucosinolate, and 4-methylsulfinylbutyl glucosinolate is 50-70% of that with allylglucosinolate, overview
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additional information
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highest myrosinase activity is detected with allylglucosinolate. Activity with 3-butenylglucosinolate, 4-pentenylglucosinolate, and 4-methylsulfinylbutyl glucosinolate is 50-70% of that with allylglucosinolate, overview
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leaves of four-week old Brassica rapa plants that have been damaged by Psylliodes chrysocephala adults for one week are analyzed for their glucosinolates profiles, overview
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the enzyme is normally physically segregated from the glucosinolates, but when the cells are damaged, e.g. during food preparation, mastication or injury by predators such as insects, the enzyme is released and catalyzes their hydrolysis of glucosinolates
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myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
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additional information
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decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
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decomposition of glucosinolates in horseradish extract inoculated by endophytic fungi from horseradish, overview
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hydrolysis of rapeseed meal yields 1-cyano-2-hydroxy-3-butene, 5-vinyloxazolidine-2-thione, 3-butenylisothiocyanate and 4-pentenylisothiocyanate
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additional information
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enzyme retains the anomeric configuration at the cleavage point, the catalytic residue is a nucleophilic glutamate
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additional information
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the enzyme does not catalyze a transglycosylation reaction either with alcohols or with other suitable glycosyl acceptors
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additional information
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hydrolysis of mustard oil glucosides to isothiocyanate, glucose and sulfate
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additional information
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myrosinase is a unique enzyme which catalyzes the hydrolysis of sulfur-containing secondary metabolites called glucosinolates
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additional information
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myrosinase is a unique enzyme which catalyzes the hydrolysis of sulfur-containing secondary metabolites called glucosinolates
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additional information
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myrosinase catalyzes hydrolysis of the S-glycosyl bond, O-beta glycosyl bond, and O-glycosyl bonds of glucosinolates
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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mainly oxazolidine-2-thione in the absence of epithiospecifier protein and mainly epithionitrile in the presence of epithiospecifier protein
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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progoitrin + H2O
additional information
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