3.2.1.147: thioglucosidase

This is an abbreviated version!
For detailed information about thioglucosidase, go to the full flat file.

Reaction

Synonyms

ArMy2, AtBGLU37, AtBGLU38, atypical myrosinase, beta-glucosidase 26, peroxisomal, beta-glucosidase 37, beta-glucosidase 38, beta-thioglucosidase, beta-thioglucosidase glucohydrolase, beta-thioglucoside glucohydrolase, beta-thioglucoside glucohydrolase,, BGLU26, CpTGG1, CpTGG2, EC 3.2.3.1, glucosidase, thio-, glucosinolase, More, MYR, MYR II, MYR1 myrosinase, Myr1.Bn1, Myr2.Bn1, MYRc, MYRI, MYRII, myrosin, myrosinase, myrosinase 1, myrosinase 2, myrosinase A, myrosinase B, PEN2, PYK10 myrosinase, sinigrase, sinigrinase, sinigrinase 1, sinigrinase 2, TGG, TGG1, TGG2, TGG4, TGG5, Thioglucosidase, thioglucosidase 1, thioglucosidase 2, thioglucoside glucohydrolase, thioglucoside glucohydrolase 1, thioglycosidase, WjMYR

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.147 thioglucosidase

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Results	in table
89	Activating Compound
979	AA Sequence
8	Application
1	CAS Registry Number
17	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
73	Disease/ Diagnostics
27	Expression
123	General Information
15	General Stability
4	IC50 Value
177	Inhibitors
11	kcat/KM [mM/s]
1	Ki Value [mM]
78	KM Value [mM]
17	Localization
41	Metals/Ions
74	Molecular Weight [Da]
81	Natural Substrates/ Products (Substrates)
279	Organism
1	Oxidation Stability
6	Pathway
11	PDB ID
74	pH Optimum
24	pH Range
17	pH Stability
11	pI Value
31	Posttranslational Modification
14	Protein Variants
67	Purification (Commentary)
3	Reaction
3	Reaction Type
256	Reference
264	Source Tissue
54	Specific Activity [micromol/min/mg]
3	Storage Stability
393	Substrates and Products (Substrate)
60	Subunits
225	Synonyms
1	Systematic Name
50	Temperature Optimum [°C]
16	Temperature Range [°C]
39	Temperature Stability [°C]
26	Turnover Number [1/s]

pH Stability

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pH Stability on EC 3.2.1.147 - thioglucosidase

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pH STABILITY

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

10

Eutrema japonicum

-

5°C, 41 h, about 25% loss of activity

208558

11

Eutrema japonicum

-

5°C, 41 h, about 30% loss of activity

208558

4

Eutrema japonicum

-

5°C, 41 h, about 60% loss of activity

208558

4.8 - 10.3

Arabidopsis thaliana

P37702, Q9C5C2

the purified recombinant enzyme TGG1 retains more than 50% of their maximal activity in buffers with pH values ranging from 4.8 to 10.3

732658

4.8 - 11

Arabidopsis thaliana

P37702, Q9C5C2

the purified recombinant enzyme TGG1 retains more than 50% of their maximal activity in buffers with pH values ranging from 4.8 to 11.0

732658

5 - 7

Enterobacter cloacae

-

below 40°C, 24 h, stable

208561

5.5 - 8.5

2 entries

6

3 entries

6 - 11

Crambe hispanica subsp. abyssinica

-

37°C, 30 min, stable

665258

6 - 9

Brassica oleracea

-

30°C, 1 h, stable

208587

7

2 entries

7.6 - 8

Aspergillus niger

-

5°C, 24 h, stable

208564

9

Aspergillus niger

-

5°C, 24 h, about 55% loss of activity

208564

5.5 - 8.5

Aspergillus sydowii

-

below 45°C, stable

208573

5.5 - 8.5

Sinapis alba

-

maximal activity is maintained in a large pH interval ranging from 5.5 to 8.5. At lower pH values, free and immobilized myrosinase forms show a strong activity loss, at pH 5.0 it is about 50% of the maximal activity, whereas it is almost absent at pH 4.5

716638

6

Brassica napus

-

maximal stability

208565

6

Eutrema japonicum

-

5°C, 41 h, about 10% loss of activity

208558

6

Sinapis alba

-

maximal stability

208565

7

Aspergillus niger

-

5°C, 24 h, about 20% loss of activity

208564

7

Eutrema japonicum

-

5°C, 41 h, stable

208558