3.2.1.147: thioglucosidase

This is an abbreviated version!
For detailed information about thioglucosidase, go to the full flat file.

Reaction

Synonyms

ArMy2, AtBGLU37, AtBGLU38, atypical myrosinase, beta-glucosidase 26, peroxisomal, beta-glucosidase 37, beta-glucosidase 38, beta-thioglucosidase, beta-thioglucosidase glucohydrolase, beta-thioglucoside glucohydrolase, beta-thioglucoside glucohydrolase,, BGLU26, CpTGG1, CpTGG2, EC 3.2.3.1, glucosidase, thio-, glucosinolase, More, MYR, MYR II, MYR1 myrosinase, Myr1.Bn1, Myr2.Bn1, MYRc, MYRI, MYRII, myrosin, myrosinase, myrosinase 1, myrosinase 2, myrosinase A, myrosinase B, PEN2, PYK10 myrosinase, sinigrase, sinigrinase, sinigrinase 1, sinigrinase 2, TGG, TGG1, TGG2, TGG4, TGG5, Thioglucosidase, thioglucosidase 1, thioglucosidase 2, thioglucoside glucohydrolase, thioglucoside glucohydrolase 1, thioglycosidase, WjMYR

ECTree

     3 Hydrolases

         3.2 Glycosylases

             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds

                3.2.1.147 thioglucosidase

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Results	in table
89	Activating Compound
979	AA Sequence
8	Application
1	CAS Registry Number
17	Cloned(Commentary)
2	Cofactor
3	Crystallization (Commentary)
73	Disease/ Diagnostics
27	Expression
123	General Information
15	General Stability
4	IC50 Value
177	Inhibitors
11	kcat/KM [mM/s]
1	Ki Value [mM]
78	KM Value [mM]
17	Localization
41	Metals/Ions
74	Molecular Weight [Da]
81	Natural Substrates/ Products (Substrates)
279	Organism
1	Oxidation Stability
6	Pathway
11	PDB ID
74	pH Optimum
24	pH Range
17	pH Stability
11	pI Value
31	Posttranslational Modification
14	Protein Variants
67	Purification (Commentary)
3	Reaction
3	Reaction Type
256	Reference
264	Source Tissue
54	Specific Activity [micromol/min/mg]
3	Storage Stability
393	Substrates and Products (Substrate)
60	Subunits
225	Synonyms
1	Systematic Name
50	Temperature Optimum [°C]
16	Temperature Range [°C]
39	Temperature Stability [°C]
26	Turnover Number [1/s]

Application

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Application on EC 3.2.1.147 - thioglucosidase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

agriculture

2 entries

analysis

2 entries

biotechnology

Aspergillus sp.

-

Aspergillus sp. NR463U4 maintains constant myrosinase production for 8 months. High production and prolonged stability of myrosinase demonstrates that this mutant could be a candidate for industrial application

663633

nutrition

2 entries

pharmacology

Brassica oleracea var. italica

A0A343IQS8

sulforaphane, the reactive isothiocyanate that potently inhibits neoplastic cellular processes and prevents a number of disease states in humans, is difficult to deliver in an enriched and stable form for purposes of direct human consumption. Evaluation of the bioavailability of sulforaphane, either by direct administration of glucoraphanin (a glucosinolate, or beta-thioglucoside-N-hydroxysulfate), or by co-administering glucoraphanin and the enzyme myrosinase to catalyze its conversion to sulforaphane at economic, reproducible and sustainable yields, overview. Following administration of glucoraphanin in a commercially prepared dietary supplement to a small number of human volunteers, the volunteers have equivalent output of sulforaphane metabolites in their urine to that which they produce when given an equimolar dose of glucoraphanin in a simple boiled and lyophilized extract of broccoli sprouts. Furthermore, when either broccoli sprouts or seeds are administered directly to subjects without prior extraction and consequent inactivation of endogenous myrosinase, regardless of the delivery matrix or dose, the sulforaphane in those preparations is 3 to 4fold more bioavailable than sulforaphane from glucoraphanin delivered without active plant myrosinase

755104

agriculture

Brassica juncea

-

larvae of the sawfly Athalia rosae are highly tolerant to variations in the glucosinolate-myrosinase system of its host Brassica juncea. In plants showing high levels of glucosinolate or of enzyme activity, a significant decrease is observed upon treatment with the larvae, whereas levels of insoluble myrosinases coverge in plant lines with high and low level of activity after feeding of larvae

679235

agriculture

Raphanus sativus

-

spraying exogenous plant hormone methyl jasmonate upon radish sprout decreases the activity of myrosinase and the amount of 4-methylthio-3-butenylisothiocyanate but increases the total phenolic content which results in increased 2,2-diphenyl-1-picrylhydrazyl free radical scavenging capacity

680285

analysis

Brassica carinata

-

micellar electrokinetic capillary chromatography method for monitoring the myrosinase catalyzed hydrolysis of 2-hydroxy substituted glucosinolates and formation of the corresponding oxazolidine-2-thiones and nitriles. Method has a detection limit of 0.04 mM and a limit of quantification of 0.2 mM and 0.3 mM for the glucosibarin-derived oxazolidine-2-thione and nitrile, respectivityl

681049

analysis

Brassica carinata BRK-147-A

-

micellar electrokinetic capillary chromatography method for monitoring the myrosinase catalyzed hydrolysis of 2-hydroxy substituted glucosinolates and formation of the corresponding oxazolidine-2-thiones and nitriles. Method has a detection limit of 0.04 mM and a limit of quantification of 0.2 mM and 0.3 mM for the glucosibarin-derived oxazolidine-2-thione and nitrile, respectivityl

-

nutrition

Brassica oleracea

-

in intact vegetable tissues, the enzyme is present in compartments separated from its substrate, the glucosinolates. The enzymatic hydrolysis can merely occur after cellular disruption. In this respect, processes such as cutting, cooking, freezing, or pressurizing of the vegetables will have large effect on the glucosinolate hydrolysis by myrosinase

665880

nutrition

Brassica oleracea

-

study on kinetics of thermal enzyme inactivation in broccoli juice at elevated pressure for optimization of health effects. Pressure has an antagonistic effect on thermal inactivation at 50°C and above. Isothiocyanates formed by enzyme are relatively thermolabile and pressure stable

680299