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Literature summary for 3.2.1.147 extracted from
- Characterization of a root-specific ?-thioglucoside glucohydrolase gene in Carica papaya and its recombinant protein expressed in Pichia pastoris (2009), Plant Sci., 177, 716-723.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | stimulates 9fold at 0.2 mM, maximally 18fold at 00.8 mM | Carica papaya |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pTGG2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Pichia pastoris | Carica papaya |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | inhibits, completely at 1 M | Carica papaya | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.24 | - |
sinigrin | pH 6.0, 37°C | Carica papaya | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59500 | - |
x * 70000, recombinant TGG2, SDS-PAGE, x * 59500, about, TGG2 sequence calculation | Carica papaya |
| 70000 | - |
x * 70000, recombinant TGG2, SDS-PAGE, x * 59500, about, TGG2 sequence calculation | Carica papaya |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Carica papaya | C9WCQ1 | cv. Solo II, gene pTGG2 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | CpTGG2 may have 5 N-glycosylation sites | Carica papaya |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant TGG2 from Pichia pastoris | Carica papaya |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | CpTGG2 is specifically expressed in the root | Carica papaya | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.3 | - |
purified recombinant enzyme | Carica papaya |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sinigrin + H2O | i.e. 2-propenyl glucosinolate, substrate saturation at 6 mM | Carica papaya | allyl isothiocyanate + D-glucose + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 70000, recombinant TGG2, SDS-PAGE, x * 59500, about, TGG2 sequence calculation | Carica papaya |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-thioglucoside glucohydrolase | - |
Carica papaya |
| More | TGGs belong to the glycosyl hydrolase family 1 | Carica papaya |
| myrosinase | - |
Carica papaya |
| TGG2 | - |
Carica papaya |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Carica papaya |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 90 | CpTGG2 is active in broad temperature range, peaks at 40°C, gradually decreases between 40°C and 90°C, and shows 13% residual activity at 80°C and 1% at 90°C | Carica papaya |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
purified recombinant TGG2, 20 min, 50% activity remaining | Carica papaya |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 28.4 | - |
sinigrin | pH 6.0, 37°C | Carica papaya | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Carica papaya |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 11 | CpTGG2 is active in broad pH range, almost inactive below pH 3.0 and above pH 13.0 | Carica papaya |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Carica papaya | TGG2 sequence calculation | - |
6.35 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 13 | - |
sinigrin | pH 6.0, 37°C | Carica papaya | |
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