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2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
2'-fucosyllactose + H2O
alpha-L-fucose + lactose
-
-
-
?
2-chloro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-chloro-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride + H2O
fluoride + 2-deoxy-2-fluoro-alpha-L-fucose
-
-
-
?
2-fluoro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-fluoro-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-methyl-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-methyl-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-nitrophenyl alpha-L-fucopyranoside + H2O
2-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
2alpha-fucosyl-lactitol + H2O
?
-
-
-
-
?
3'-fucosyllactose + H2O
alpha-L-fucose + ?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
3-fucosyllactose + H2O
L-fucose +
3-fucosyllactose + H2O
L-fucose + ?
-
cleavage of alpha-1,3 bond
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucopyranose
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-beta-fucoside
?
-
-
-
-
?
4-nitrophenyl 2-sulfo-alpha-L-fucopyranoside + H2O
4-nitrophenol + 2-sulfo-alpha-L-fucopyranose
-
hydrolysis at 1.9% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
-
hydrolysis at 1.6% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
sodium acetate buffer 50 mM, pH 5.0, 37°C
-
-
?
4-nitrophenyl alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside
?
-
assay at pH 4.5, 37°C for 3 h, reaction stopped by addition of borate buffer
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-fucose-(1-3)-D-GlcNAc
-
catalytic residues Asp242, Glu58, and Glu292
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
4-nitrophenyl-alpha-L-fucoside + H2O
alpha-L-fucopyranose + 4-nitrophenol
-
-
-
-
?
6-O-alpha-L-Fuc-N,N'-diacetylchitobiose + H2O
alpha-L-fucose + N,N'-diacetylchitobiose
-
-
-
?
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-a-L-Fuc-O-4-NP + H2O
?
-
-
-
-
?
alpha-L-Fuc-(1-3)-alpha-L-Fuc-O-4-nitrophenyl ester + H2O
?
-
-
-
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-3)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
alpha-L-Fuc-(1-4)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-6)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe + H2O
alpha-L-fucose + beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe
-
-
-
?
alpha-L-Fuc-(1->4)-GlcNAc-poly-acrylamide polymer + H2O
alpha-L-fucose + GlcNAc-poly-acrylamide polymer
-
-
-
?
alpha-L-fucopyranosyl fluoride + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
-
-
13% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + N-acetylglucosamine
2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranose + fluoride
-
-
7% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
-
-
8% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + fluoride
-
-
34% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
-
-
?
alpha-L-fucopyranosyl-(1->2)-D-galactopyranose + H2O
alpha-L-fucose + D-galactose
the enzyme does not hydrolyze other fucosyloligosaccharides or 4-nitrophenyl-alpha-L-fucoside
-
-
?
alpha-L-fucosyl fluoride + H2O
alpha-L-fucose + fluoride
alpha-L-fucosyl fluoride + H2O
fluoride + alpha-L-fucose
-
-
-
?
antigen H disaccharide + H2O
?
-
-
-
-
?
antigen H type II trisaccharide + H2O
?
-
-
-
-
?
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GlcNAc
slightly hydrolyzed, since fucose monosaccharide is detected after the reaction
-
-
?
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1->3)-D-GlcNAc
i.e. Le(x) trisaccharide
-
-
?
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-OMe + H2O
?
-
-
-
?
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1->4)-D-GlcNAc
i.e. Le(a) trisaccharide
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
alpha-L-fucose + Fuc-beta-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
alpha-L-fucose + beta-galactosyl-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-Glc + H2O
alpha-L-fucose + D-glucose
-
-
-
?
Fuc-alpha-(1-2)-Xyl + H2O
alpha-L-fucose + D-xylose
-
-
-
?
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
-
-
-
?
Fuc-alpha-(1-3)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Fuc-alpha-(1-3)-Glc + H2O
alpha-L-fucose + D-glucose
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
-
-
-
?
Fuc-alpha-(1-6)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
alpha-L-fucose + GlcNAc-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
-
-
?
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
-
-
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
low activity
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
Bacillus fulminans
-
-
-
?
fucoidan + H2O
alpha-L-fucose + ?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
gastric mucin + H2O
alpha-L-fucose + ?
GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues
-
?
glycopeptide + H2O
alpha-L-fucose + ?
glycopeptide from desialyzed porcine submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
-
?
glycoprotein + H2O
alpha-L-fucose + ?
glycosphingolipid + H2O
alpha-L-fucose + ?
-
blood-group active types B and H
-
?
gp120 oligosaccharide + H2O
alpha-L-fucose + ?
-
-
-
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
IgG Fc peptide + H2O
alpha-L-fucose + ?
IgG L peptide + H2O
alpha-L-fucose + ?
-
-
-
?
lacto-N-difucohexaose I + H2O
L-fucose + ?
-
cleavage of alpha-1,4 bond
-
-
?
lacto-N-difucohexaose II + H2O
L-fucose + ?
-
cleavage of alpha-1,4 bond
-
-
?
lacto-N-fucopentaitol II + H2O
alpha-L-fucose + ?
-
-
-
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
lacto-N-fucopentaose + H2O
L-fucose +
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
lactoferrin + H2O
alpha-L-fucose + ?
-
low activity
-
?
LH-beta peptic glycopeptide + H2O
alpha-L-fucose + ?
-
-
-
?
m-nitrophenyl alpha-L-fucopyranoside + H2O
m-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
-
-
?
meconium H-1 + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
?
meconium H-2 + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
?
meconium X-1 + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
low activity
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
milk + H2O
alpha-L-fucose + ?
-
human
-
?
mono-sialylated-galactosylated biantennary N-glycan-core-substituted with alpha-(1->6)-linked fucose + H2O
L-fucose + ?
-
-
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
oligosaccharides + H2O
alpha-L-fucose + ?
orosomucoid + H2O
alpha-L-fucose + ?
-
low activity, higher activity if desialyzed
-
?
p-cyanophenyl alpha-L-fucopyranoside + H2O
p-cyanophenol + L-fucose
pH 6.8, 37°C
-
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
p-nitrophenyl alpha-L-fucopyranoside + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
29% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
34% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + p-nitrophenol
-
-
25% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
p-nitrophenyl-alpha-L-fucoside + H2O
L-fucose + p-nitrophenol
the enzyme has low transglycosylation properties
-
-
?
phenyl alpha-L-fucopyranoside + H2O
phenol + L-fucose
pH 6.8, 37°C
-
-
?
porcine submaxillary mucine, desialyzed + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
-
?
propyl alpha-L-fucopyranoside + H2O
propanol + alpha-L-fucopyranose
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside + H2O
?
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside + H2O
?
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside + H2O
?
-
-
-
?
saliva + H2O
alpha-L-fucose + ?
stomach + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
?
submaxilary mucin trisaccharide + H2O
alpha-L-fucose + disaccharide
-
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
thyroglobulin + H2O
alpha-L-fucose + ?
Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside
-
-
-
?
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Fucalpha(1-2)Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + H2O
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + alpha-L-fucose
-
-
-
-
?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
additional information
?
-
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
-
-
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
-
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase II highly active
-
?
3'-fucosyllactose + H2O
alpha-L-fucose + ?
-
-
-
-
?
3'-fucosyllactose + H2O
alpha-L-fucose + ?
-
-
-
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
very low activity
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
3-fucosyllactose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond, low efficiency
-
-
?
3-fucosyllactose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond, low efficiency
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucopyranose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucopyranose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
induces acrosome reaction of guinea pig spermatozoa
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
responsible for the intralysosomal hydrolysis of fucose-containing glypeptide and glycolipids
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
involved in sperm-egg interactions
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
-
hydrolysis at 1.7% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
-
hydrolysis at 3.7% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
the enzyme shows a low transglycosylation potential
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
the enzyme shows a low transglycosylation potential
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
the enzyme shows a low transglycosylation potential
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
WP_057784261.1
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
WP_057784261.1
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
substrate incubated with plasma sample of breast cancer patients treated with anti-cancer monoclonal antibody trastuzumbal
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
-
transglycosylation activity
no other isomer
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
-
transglycosylation activity
no other isomer
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
-
?
alpha-L-fucosyl fluoride + H2O
alpha-L-fucose + fluoride
-
-
-
-
?
alpha-L-fucosyl fluoride + H2O
alpha-L-fucose + fluoride
-
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Bacillus fulminans
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
only hydrolysed by alpha-L-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Bacillus fulminans
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
only hydrolysed by alpha-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc, alpha-(1-3)-GlcNAc or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
NaBH4-reduced oligosaccharide, hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
fucoidan + H2O
alpha-L-fucose + ?
-
-
-
-
?
fucoidan + H2O
alpha-L-fucose + ?
-
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
-
fase I, not: fase II
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
-
fase I, not: fase II
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity for alpha-L-fucosidase II
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
NaBH4-reduced oligosaccharide, very slow hydrolysis
-
?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
very slow hydrolysis, NaBH4-reduced oligosaccharide
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
low activity
-
?
gastric mucin + H2O
alpha-L-fucose + ?
-
porcine
-
?
gastric mucin + H2O
alpha-L-fucose + ?
-
porcine
-
?
gastric mucin + H2O
alpha-L-fucose + ?
-
porcine
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
from meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillry mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillary mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
cellular source of substrate
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
not active on native glycoproteins
-
?
glycoprotein + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
-
?
glycoprotein + H2O
alpha-L-fucose + ?
-
salivary human type H
-
?
IgG Fc peptide + H2O
alpha-L-fucose + ?
-
from human, rabbit, horse
-
?
IgG Fc peptide + H2O
alpha-L-fucose + ?
-
human, rabbit, horse
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
-
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
low activity for alpha-L-fucosidase II
-
?
lacto-N-fucopentaose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond
-
-
?
lacto-N-fucopentaose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond, low efficiency
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
preferentially hydrolysed by alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
-
or methanol or ethanol as acceptor
-
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
-
or methanol or ethanol as acceptor
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
-
-
anomeric configuration of the produced fucopyranse
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
-
or methanol or ethanol as acceptor
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
saliva + H2O
alpha-L-fucose + ?
-
Ulex europaeus
-
?
saliva + H2O
alpha-L-fucose + ?
-
human
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed and not, desialyzed mucine best substrate tested
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
one form, optimally active at pH 5, not able to hydrolyse p-nitrophenyl-alpha-L-fucopyranoside
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
bovine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
from bovine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
hydrolysis only after pronase or neuraminidase treatment
-
?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
-
-
?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
subscript OT is used to indicate NaBH4-reduced oligosaccharide
-
?
additional information
?
-
-
not: p-nitrophenyl-alpha-L-fucoside
-
-
?
additional information
?
-
-
series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis
-
-
?
additional information
?
-
-
series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
Afc2 shows enzyme activities exclusively to Le(x) trisaccharide and Le(a) trisaccharide. Although 3-fucosyl-GlcNAc and 4-fucosyl-GlcNAc have the same fucosyl linkage with Le(x) or Le(a) trisaccharides, they are resistant to the hydrolysis by Afc2 because they do not have the branched Gal residues at the non-reducing end. Afc2 is a 1,3-1,4-alphs-L-fucosidase
-
-
?
additional information
?
-
Afc2 shows enzyme activities exclusively to Le(x) trisaccharide and Le(a) trisaccharide. Although 3-fucosyl-GlcNAc and 4-fucosyl-GlcNAc have the same fucosyl linkage with Le(x) or Le(a) trisaccharides, they are resistant to the hydrolysis by Afc2 because they do not have the branched Gal residues at the non-reducing end. Afc2 is a 1,3-1,4-alphs-L-fucosidase
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency
-
-
?
additional information
?
-
-
the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively
-
-
?
additional information
?
-
-
the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides
-
-
?
additional information
?
-
-
the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency
-
-
?
additional information
?
-
-
the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively
-
-
?
additional information
?
-
-
the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides
-
-
?
additional information
?
-
the enzyme preferentially hydrolyzes the alpha-(1->4)-L-fucosidic linkage present in alpha-L-fucobiosides with different types of linkages. The enzyme is capable of catalyzing the transglycosylation reaction with alcohols (methanol, ethanol, and 1-propanol) and 4-nitrophenyl-containing monosaccharides as acceptors
-
-
?
additional information
?
-
-
the enzyme preferentially hydrolyzes the alpha-(1->4)-L-fucosidic linkage present in alpha-L-fucobiosides with different types of linkages. The enzyme is capable of catalyzing the transglycosylation reaction with alcohols (methanol, ethanol, and 1-propanol) and 4-nitrophenyl-containing monosaccharides as acceptors
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
no substrate: unable to hydrolyze 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl N-acetyl-beta-D-glucosaminide, lactose, melibiose, lactulose, maltose, or maltotriose
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
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additional information
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all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
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?
additional information
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the enzyme is capable of transglycosylation on a variety of acceptor molecules, including saccharides, alcohols or chromogenic substrates
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additional information
?
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all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
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?
additional information
?
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the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan
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?
additional information
?
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Two specific mutations, designed on the basis of the programmed -1 frameshifting mechanism, promotes transfucosylation reactions by following a retaining reaction mechanism.
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additional information
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4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-glucoside, 4-nitrophenyl-alpha-D-xyloside, 4-nitrophenyl-alpha-D-galactoside and 4-nitrophenyl-alpha-D-mannoside are not substrates for wild-type and mutant fucA1B
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?
additional information
?
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all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
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?
additional information
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TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
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additional information
?
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TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
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?
additional information
?
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TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
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?
additional information
?
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all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
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?
additional information
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behaviour of the catalytic residues is different from that of Sulfolobus solfataricus alpha-fuc
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additional information
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the wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a 4-nitrophenyl-fucoside donor to 4-nitrophenyl-fucoside (self-condensation) with alpha-(1,3) regioselectivity or 4-nitrophenyl-galactoside (transglycosylation) with alpha-(1,2) regioselectivity at low yields (7%)
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additional information
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the wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a 4-nitrophenyl-fucoside donor to 4-nitrophenyl-fucoside (self-condensation) with alpha-(1,3) regioselectivity or 4-nitrophenyl-galactoside (transglycosylation) with alpha-(1,2) regioselectivity at low yields (7%)
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additional information
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in the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor, the presence of 1.1 M CaCl2 favors the rate of transfucosylation, and improves the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 mM and 146 mM, respectively. CaCl2 does not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in water activity and lactose concentration has a synergistic effect favoring the synthesis of fucosylated oligosaccharides
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additional information
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not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
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additional information
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not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
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additional information
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Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
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additional information
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Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
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additional information
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Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
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additional information
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Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
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additional information
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Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
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additional information
?
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Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
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additional information
?
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Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
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additional information
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the enzyme can hydrolyze the partially degraded fragment of fucoidan. No free fucose can be released from alpha-Fuc-(1->2)-beta-Gal-(1->4)-Glc, beta-Gal-(1->4)-[alpha-Fuc-(1->3)]-Glc or alpha-Fuc-(1->6)-GlcNAc
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additional information
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the enzyme can hydrolyze the partially degraded fragment of fucoidan. No free fucose can be released from alpha-Fuc-(1->2)-beta-Gal-(1->4)-Glc, beta-Gal-(1->4)-[alpha-Fuc-(1->3)]-Glc or alpha-Fuc-(1->6)-GlcNAc
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2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
-
-
-
?
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
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?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
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?
Fuc-alpha-(1-2)-Glc + H2O
alpha-L-fucose + D-glucose
-
-
-
?
Fuc-alpha-(1-2)-Xyl + H2O
alpha-L-fucose + D-xylose
-
-
-
?
Fuc-alpha-(1-3)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
Fuc-alpha-(1-3)-Glc + H2O
alpha-L-fucose + D-glucose
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
-
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
low activity
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
Bacillus fulminans
-
-
-
?
fucoidan + H2O
alpha-L-fucose + ?
-
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
very slow hydrolysis, NaBH4-reduced oligosaccharide
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
gastric mucin + H2O
alpha-L-fucose + ?
glycopeptide + H2O
alpha-L-fucose + ?
glycoprotein + H2O
alpha-L-fucose + ?
glycosphingolipid + H2O
alpha-L-fucose + ?
-
blood-group active types B and H
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?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
IgG Fc peptide + H2O
alpha-L-fucose + ?
-
human, rabbit, horse
-
?
IgG L peptide + H2O
alpha-L-fucose + ?
-
-
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
lactoferrin + H2O
alpha-L-fucose + ?
-
low activity
-
?
LH-beta peptic glycopeptide + H2O
alpha-L-fucose + ?
-
-
-
?
meconium H-1 + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
?
meconium H-2 + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
?
meconium X-1 + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
low activity
-
?
milk + H2O
alpha-L-fucose + ?
-
human
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
orosomucoid + H2O
alpha-L-fucose + ?
-
low activity, higher activity if desialyzed
-
?
saliva + H2O
alpha-L-fucose + ?
submaxillary mucin + H2O
alpha-L-fucose + ?
thyroglobulin + H2O
alpha-L-fucose + ?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
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?
additional information
?
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the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan
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?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
-
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase II highly active
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
very low activity
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Bacillus fulminans
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
only hydrolysed by alpha-L-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Bacillus fulminans
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
only hydrolysed by alpha-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity for alpha-L-fucosidase II
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
low activity
-
?
gastric mucin + H2O
alpha-L-fucose + ?
-
porcine
-
?
gastric mucin + H2O
alpha-L-fucose + ?
-
porcine
-
?
gastric mucin + H2O
alpha-L-fucose + ?
-
porcine
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillary mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
cellular source of substrate
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
not active on native glycoproteins
-
?
glycoprotein + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
-
?
glycoprotein + H2O
alpha-L-fucose + ?
-
salivary human type H
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
-
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
low activity for alpha-L-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
preferentially hydrolysed by alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
saliva + H2O
alpha-L-fucose + ?
-
Ulex europaeus
-
?
saliva + H2O
alpha-L-fucose + ?
-
human
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed and not, desialyzed mucine best substrate tested
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
one form, optimally active at pH 5, not able to hydrolyse p-nitrophenyl-alpha-L-fucopyranoside
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
-
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
bovine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
hydrolysis only after pronase or neuraminidase treatment
-
?
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(1R,2R,3R,4R,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
(1R,2R,3R,4S,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
(2S,3R,4R,5R)-5-(3-aminopropoxy)-2-methylpiperidine-3,4-diol
-
(2S,3R,4R,5R,6S)-2-(hydroxymethyl)-6-methylpiperidine-3,4,5-triol
-
(2S,3R,4S,5R)-1-(3-aminopropyl)-2-methylpiperidine-3,4,5-triol
-
(2S,3R,4S,5R)-2-(propan-2-yl)piperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
(2S,3R,4S,5R)-2-isopropylpiperidine-3,4,5-triol
-
(2S,3R,4S,5S)-2-methyl-5-(phenylamino)pyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-(1H-benzimidazol-2-yl)-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-[(biphenyl-4-ylamino)methyl]-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(5R,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 50% inhibition
(5R,6S,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 41% inhibition
(5S,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 49% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 55% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]-4-azaspiro[2.4]heptane
-
1 mM,97 inhibition, competitive
1,5-dideoxy-1,5-([(2R,3R)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2R,3S,4R,5R)-2,4,5,6-tetrahydroxy-3-(sulfooxy)hexyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-[5-methoxycarbonyl-1-pentyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1,5-dideoxy-1,5-[methyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
-
complete inactivation at 22 mM
1-deoxygalactonojirimycin
-
-
1-deoxymannojirimycin
-
-
2-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-(hydroxymethyl)-5-methylpyrrolidine-3,4-diol
-
competitive or uncompetitive inhibition, Ki: 0.004-26 mM, depending on conformation
2-cyclopentyl-2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)acetamide
-
2-cyclopentyl-2-phenyl-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-methyltetrahydro-2H-pyran-3,4-diol
-
uncompetitive inhibition, Ki: 23 mM
2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)quinoline-4-carboxamide
-
2-phenyl-tetrahydro-2H-pyran-3,4-diol
-
uncompetitive inhibition, Ki: 22-83 mM, depending on conformation
3-(1H-indol-3-yl)-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)propanamide
-
3-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]propanamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
4-aminophenyl 1-thio alpha-L-fucoside
-
weak competitive inhibition, Ki: 1.25 mM
4-chloromercuriphenylsulfonic acid
4-hydroxy-2-methyltetrahydro-2H-pyran-3-yl acetate
-
competitive inhibition, Ki: 0.09 mM
4-mercuribenzoate
-
complete inhibition at 0.25 mM
4-nitrophenyl alpha-L-fucopyranoside
substrate inhibition at high concentrations
5-(hydroxymethyl)pyrrolidine-2,3,4-triol
-
competitive inhibition, Ki: 0.91 mM
5-amino-5-deoxy-L-fucose
-
-
5-fluoro-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)-1H-indole-2-carboxamide
-
6-N-acetylamino-deoxymannojirimycin
-
-
Ag2+
-
complete inactivation at 1 mM
alpha-L-fuco-N-benzyl aminocyclopentitol
Co2+
1 mM, 61% loss of activity
D-1-deoxymannojirimycin
-
competitive inhibition, Ki: 0.03 mM
D-1-deoxyrhamnojirimycin
-
competitive inhibition, Ki: 0.011 mM
D-1-deoxytalonojirimycin
-
competitive inhibition, Ki: 0.053 mM
D-fucal
-
uncompetitive inhibition, Ki: 35 mM
D-fucose
-
15% inhibition at 50 mM
D-galactono-1,4-lactone
-
15% inhibition at 50 mM
D-glucono-1,5-lactone
-
30% inhibition at 50 mM
ethanolamine
-
strong inhibition
ethylenediaminetetraacetic acid
Bacillus fulminans
-
68% inhibition at 0.1 mM
Fe3+
-
40% inhibition at 2 mM
formaldehyde
-
57% inhibition at 160 mM
fucosylamine
-
competitive inhibition, Ki: 0.014 mM
iodoacetamide
-
30% inhibition at 1 mM
L-1-deoxyfuconojirimycin
-
competitive inhibition, Ki: 0.00003 mM
L-1-deoxymannojirimycin
-
competitive inhibition, Ki: 90 mM
L-1-deoxyrhamnojirimycin
-
competitive inhibition, Ki: 1 mM
L-fucal
-
competitive inhibition, best inhibitor of glycal compounds tested, Ki: 0.05 mM
L-fuco-nojirimycin
-
very potent, competitive inhibitor
L-galactono-1,4-lactone
-
30% inhibition at 50 mM
L-rhamnose
-
competitive inhibition, Ki: 140 mM
Mg2+
-
slight inhibition at 10 mM
N-ethylmaleimide
-
30% inhibition at 1 mM
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1-benzofuran-2-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1H-indole-3-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
-
p-nitrophenyl alpha-L-fucoside
substrate inhibition at high concentration
Pb2+
-
effective inhibition at 5 mM
phenol
-
noncompetitive inhibition, Ki: 112 mM
SDS
Bacillus fulminans
-
42% inhibition at 0.02%, w/v
Sn2+
-
24% inhibition at 2 mM
Tris(hydroxymethyl)aminomethane
-
Zn-acetate
-
more than 90% inhibition at 5 mM
(1R,2R,3R,4R,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(1R,2R,3R,4R,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(1R,2R,3R,4R,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(1R,2R,3R,4S,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(1R,2R,3R,4S,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(1R,2R,3R,4S,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
-
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
-
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(R)-2-aminobutanol
-
-
(S)-2-aminobutanol
-
-
1-deoxyfuconojirimycin
-
-
1-deoxyfuconojirimycin
-
-
4-chloromercuribenzoate
-
92% inhibition at 0.1 mM
4-chloromercuribenzoate
-
50% inhibition at 0.0015 mM, totally reversal by addition of 4 mM DTT
4-chloromercuribenzoate
-
71% inhibition at 1 mM
4-chloromercuriphenylsulfonic acid
-
slight inhibition at 1 mM
4-chloromercuriphenylsulfonic acid
-
complete inhibition at 0.002 mM
Ag+
-
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
Ag+
-
59% inhibition at 3 mM
Ag+
-
effective inhibition at 5 mM
alpha-L-fuco-N-benzyl aminocyclopentitol
-
-
alpha-L-fuco-N-benzyl aminocyclopentitol
-
-
alpha-L-fuco-N-benzyl aminocyclopentitol
-
-
alpha-L-fucose
-
-
alpha-L-fucose
-
competitive inhibition, Ki: 0.3 mM
alpha-L-fucose
-
competitive inhibition at 50 mM
alpha-L-fucose
-
competitive inhibition, Ki: 0.065 mM
alpha-L-fucose
-
90% competitive inhibition at 5 mM, Ki: 0.12 mM
alpha-L-fucose
-
85% inhibition at 2.5 mM
alpha-L-fucose
competitive
alpha-L-fucose
-
competitive inhibition at 50 mM
alpha-L-fucose
-
competitive inhibition, Ki: 0.275 mM
alpha-L-fucose
-
competitive inhibition at 50 mM
alpha-L-fucose
-
competitive inhibition, Ki: 1.6 mM
alpha-L-fucose
-
competitive inhibition, Ki: 0.285 mM
alpha-L-fucose
-
competitive inhibition, Ki: 1.6 mM
alpha-L-fucose
-
competitive inhibition, Ki: 0.13 mM
alpha-L-fucose
competitive
alpha-L-fucose
-
competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis
Ca2+
-
1 mM, 47% loss of activity of Eo3066
Ca2+
-
alpha-fucosidase I, slight inhibition at 10 mM
Cd2+
-
effective inhibition at 5 mM
Cd2+
1 mM, complete inactivation
Cu2+
-
88% inhibition at 2 mM
Cu2+
-
complete inactivation at 1 mM
Cu2+
-
effective inhibition at 5 mM
D-arabinose
-
95% inhibition at 2.5 mM
D-arabinose
-
competitive inhibition, Ki: 50 mM
D-galactose
-
15% inhibition at 50 mM
D-galactose
-
competitive inhibition, Ki: 1.7 mM
D-glucose
-
15% inhibition at 50 mM
D-glucose
-
21% inhibition at 6 mM
deoxycholate
Bacillus fulminans
-
34% inhibition at 0.2%, w/v
deoxycholate
-
36% inhibition at 0.012%, w/v
deoxyfuconojirimycin
-
competitive inhibition, Ki: 0.000024 mM
deoxyfuconojirimycin
-
inhibits by 94-100% at 1 mM
deoxyfuconojirimycin
competitive
deoxyfuconojirimycin
competitively
fuconojirimycin
majority of C1-substituted fuconojirimycins act as reversible inhibitors of the human fucosidase
fuconojirimycin
-
majority of C1-substituted fuconojirimycins are slow tight-binding inhibitors of the Thermotoga enzyme, Tyr64 and Tyr267 are critically involved in closely interacting with the aglycon of inhibitors
Hg2+
Bacillus fulminans
-
marked inhibition at 0.1 mM
Hg2+
-
effective non-competitive inhibition at 2 mM
Hg2+
-
complete inhibition at 1 mM
Hg2+
-
marked inhibition at 20 mM
Hg2+
-
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
Hg2+
-
92% inhibition at 0.1 mM
Hg2+
-
effective non-competitive inhibition at 2 mM
Hg2+
-
complete inhibition at 1 mM
Hg2+
-
potent inhibitor at 1 mM
Hg2+
-
effective non-competitive inhibition at 2 mM
Hg2+
-
effective inhibition at 5 mM
Hg2+
1 mM, complete inactivation
L-cysteine
-
20% inhibition at 30 mM
L-cysteine
-
alpha-fucosidase I, slight inhibition at 10 mM
L-fucose
-
-
Mn2+
Bacillus fulminans
-
slight inhibition at 0.1 mM
Mn2+
-
1 mM, 39% loss of activity of Eo3066
Mn2+
-
26% inhibition at 2 mM
Ni2+
-
-
Ni2+
-
effective inhibition at 5 mM
Zn2+
-
1 mM, 42% loss of activity of Eo3066
Zn2+
1 mM, complete inactivation
additional information
WP_057784261.1
the enzyme is inhibited by metabolites in the aqueous ethanol extract of Fucus evanescens
-
additional information
-
time-associated loss in the activity of alpha-fucosidase post infection. Sialic acid release is correlated with a gradual loss in activity of the enzyme
-
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0.67 - 1.18
2'-fucosyllactitol
0.63 - 20
2'-fucosyllactose
0.063
2-chloro-4-nitrophenyl alpha-L-fucopyranoside
mutant E289G
2.1
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride
pH 5.0, 60°C
0.27 - 5.2
2-nitrophenyl alpha-L-fucopyranoside
1.25 - 1.9
3'-fucosyllactose
0.0484 - 0.85
4-methylumbelliferyl alpha-L-fucopyranoside
0.022 - 0.4
4-methylumbelliferyl-alpha-L-fucopyranoside
0.00325 - 0.01825
4-methylumbelliferyl-beta-fucoside
0.071 - 3.3
4-nitrophenyl alpha-L-fucopyranoside
0.02 - 0.31
4-nitrophenyl alpha-L-fucoside
0.02 - 17
4-nitrophenyl-alpha-L-fucopyranoside
0.0287 - 5.8
4-nitrophenyl-alpha-L-fucoside
3
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc
-
pH 6.5, 37°C
7
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
pH 6.5, 37°C
6.01
alpha-L-fucopyranosyl-(1->2)-D-galactopyranose
pH 7, 37°C
0.075 - 2.5
alpha-L-fucosyl fluoride
3.1
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc
pH 7, 37°C
2.46
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc
pH 7, 37°C
9
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc
-
pH 6.5, 37°C
2.9
Fuc-alpha-(1-2)-Gal
-
-
1.56
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc
-
-
0.52
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol
-
-
1.63
Fuc-alpha-(1-3)-GlcNAc-phenol
-
-
2.01
Fuc-alpha-(1-4)-GlcNAc-beta-phenol
-
-
1.75
Fuc-alpha-(1-6)-GlcNAc
-
at pH 5.0
1.6
glycopeptide from desialyzed porcine submaxillary mucin
Bacillus fulminans
-
-
-
1.02
gp120 oligosaccharide
-
at pH 5.0
-
0.1
lacto-N-fucopentaitol II
-
alpha-fucosidase I
0.095
lacto-N-fucopentaitol III
-
alpha-fucosidase I
16
methyl alpha-L-fucopyranoside
-
pH 6.5, 37°C
0.11 - 1.06
p-nitrophenyl alpha-L-fucopyranoside
0.028 - 2.1
p-nitrophenyl alpha-L-fucoside
0.33
porcine submaxillary mucine, desialyzed
Bacillus fulminans
-
-
-
1.67
propyl alpha-L-fucopyranoside
pH 5.5, 37°C
1.23
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside
pH 5.5, 37°C
3
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside
pH 5.5, 37°C
0.53
propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside
pH 5.5, 37°C
0.67
2'-fucosyllactitol
-
alpha-fucosidase II
1.18
2'-fucosyllactitol
-
-
0.63
2'-fucosyllactose
-
-
1.79
2'-fucosyllactose
-
at pH 5.0
2.5
2'-fucosyllactose
Bacillus fulminans
-
-
10.6
2'-fucosyllactose
-
at pH 7.0
19
2'-fucosyllactose
mutant enzyme W392A, pH 8, 45°C
20
2'-fucosyllactose
wild-type enzyme, pH 8, 45°C
0.27
2-nitrophenyl alpha-L-fucopyranoside
-
pH 7.0, 37°C
2.9
2-nitrophenyl alpha-L-fucopyranoside
-
pH 7.0, 37°C
5.2
2-nitrophenyl alpha-L-fucopyranoside
-
pH 7.0, 37°C
1.25
3'-fucosyllactose
-
-
1.9
3'-fucosyllactose
-
at pH 5.0
0.0484
4-methylumbelliferyl alpha-L-fucopyranoside
-
30°C, pH not specified in the publication
0.06
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 7.0, 37°C
0.06
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 5.0, 37°C, undifferentiated HT-29 cells
0.07
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 5.0, 37°C, differentiated HT-29 cells
0.08
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 7.0, 37°C
0.85
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 5.0, 37°C
0.022
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.031
4-methylumbelliferyl-alpha-L-fucopyranoside
-
at pH 7.0
0.05
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.05 - 0.06
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.05 - 0.06
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.07
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.07 - 0.08
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.072
4-methylumbelliferyl-alpha-L-fucopyranoside
-
at pH 5.0
0.08
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.083
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.12
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.125
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.125
4-methylumbelliferyl-alpha-L-fucopyranoside
-
alpha-fucosidase I
0.14
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.14
4-methylumbelliferyl-alpha-L-fucopyranoside
-
4-5fold increase in fucosidosis patients
0.22
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.3
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.4
4-methylumbelliferyl-alpha-L-fucopyranoside
-
-
0.00325
4-methylumbelliferyl-beta-fucoside
-
control semen
0.01825
4-methylumbelliferyl-beta-fucoside
-
infected semen
0.071
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH 7.0
0.11
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH 7.0
0.13
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH 7.0
0.14
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH 7.0
0.28
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH 6.0
0.31
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH 6.0
0.4
4-nitrophenyl alpha-L-fucopyranoside
30°C, pH 9.0
0.52
4-nitrophenyl alpha-L-fucopyranoside
pH 7.0, 37°C
0.55
4-nitrophenyl alpha-L-fucopyranoside
-
30°C, pH not specified in the publication
0.67
4-nitrophenyl alpha-L-fucopyranoside
22°C, pH 9.0
1.1
4-nitrophenyl alpha-L-fucopyranoside
pH 5.5, 37°C
3.3
4-nitrophenyl alpha-L-fucopyranoside
pH 7.0, 30°C
0.02
4-nitrophenyl alpha-L-fucoside
wild-type, at 40°C
0.06
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A, at 70°C. Mutant T264A/Y267F/L322P, at 40°C
0.065
4-nitrophenyl alpha-L-fucoside
mutant T264A, at 40°C
0.07
4-nitrophenyl alpha-L-fucoside
mutant T264A, at 70°C
0.079
4-nitrophenyl alpha-L-fucoside
wild-type, at 70°C
0.08
4-nitrophenyl alpha-L-fucoside
mutant G226S/Y237H/T264A/L322P, at 40°C
0.09
4-nitrophenyl alpha-L-fucoside
mutant G226S/T264A/Y267F/L322P, at 40°C
0.09
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A/Y267F, at 40°C
0.09
4-nitrophenyl alpha-L-fucoside
mutant P196L/Y237H/Y267F, at 40°C
0.1
4-nitrophenyl alpha-L-fucoside
mutant P196L/Y237H/Y267F and mutant G226S/Y237H/T264A/L322P, at 70°C. Mutant M210V/Y237H/T264A, at 40°C
0.13
4-nitrophenyl alpha-L-fucoside
mutant R147G/L322P, at 40°C
0.14
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A/Y267F, at 70°C
0.14
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A/Y267F/L322P, at 40°C
0.19
4-nitrophenyl alpha-L-fucoside
mutant T264A/L322P, at 40°C
0.23
4-nitrophenyl alpha-L-fucoside
mutant Y237H/Y267F/L322P, at 70°C
0.25
4-nitrophenyl alpha-L-fucoside
mutant T264A/L322P7 and mutant M210V/Y237H/T264A/Y267F/L322P, at 70°C
0.28
4-nitrophenyl alpha-L-fucoside
mutant R147G/L322P, at 70°C
0.29
4-nitrophenyl alpha-L-fucoside
mutant Y237H/Y267F/L322P, at 40°C
0.3
4-nitrophenyl alpha-L-fucoside
mutant G226S/T264A/Y267F/L322P, at 70°C
0.31
4-nitrophenyl alpha-L-fucoside
mutant T264A/Y267F/L322P, at 70°C
0.02
4-nitrophenyl-alpha-L-fucopyranoside
40°C, wild-type enzyme
0.028
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type enzyme
0.028
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, wild-type enzyme
0.028
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type, at pH 6.3, in the presence of sodium phosphate
0.033
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme D146G
0.033
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D146G
0.033
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme D146G
0.056
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme E292G
0.06
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A/Y267F/L322P
0.06
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme M210V/Y237H/T264A
0.06
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E292G, at pH 6.3, in the presence of sodium phosphate
0.06
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme E292G
0.065
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A
0.07
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.07
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A
0.079
4-nitrophenyl-alpha-L-fucopyranoside
70°C, wild-type enzyme
0.08
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme G226S/Y237H/T264A/L322P
0.088
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme D124G
0.09
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme G226S/T264A/Y267F/L322P
0.09
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A/Y267F
0.09
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme P196L/Y237H/Y267F
0.09
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D124G
0.09
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E292G, at pH 4.6, in the presence of sodium acetate
0.09
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme D124G
0.1
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A
0.1
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme G226S/Y237H/T264A/L322P4
0.1
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme P196L/Y237H/Y267F
0.13
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme R147G/L322P
0.14
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.14
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A/Y267F/L322P
0.14
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme M210V/Y237H/T264A/Y267F
0.168
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.18
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.19
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.19
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A/L322P
0.21
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.22
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.22
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.23
4-nitrophenyl-alpha-L-fucopyranoside
-
isoform F2
0.23
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme Y237H/Y267F/L322P
0.242
4-nitrophenyl-alpha-L-fucopyranoside
-
isoform F1
0.25
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme M210V/Y237H/T264A/Y267F/L322P
0.25
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A/L322P
0.26
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.26
4-nitrophenyl-alpha-L-fucopyranoside
-
pH5.0, 65°C, wild-type enzyme
0.26
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type, at pH 5.0, in the presence of sodium citrate
0.277
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.28
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme R147G/L322P
0.29
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme Y237H/Y267F/L322P
0.3
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.3
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.3
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme G226S/T264A/Y267F/L322P
0.31
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A/Y267F/L322P
0.358
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.38
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.43
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.45
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.48
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.6
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.6
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium acetate
0.71
4-nitrophenyl-alpha-L-fucopyranoside
-
sperm-bound enzyme
0.75
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.5, 37°C
0.87
4-nitrophenyl-alpha-L-fucopyranoside
-
-
0.91
4-nitrophenyl-alpha-L-fucopyranoside
-
gonad fluid enzyme
1.1
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium formate
1.2
4-nitrophenyl-alpha-L-fucopyranoside
-
-
1.6
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium citrate
2.9
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 6.3, in the presence of sodium phosphate and NaN3
4.37
4-nitrophenyl-alpha-L-fucopyranoside
-
pH5.0, 65°C, mutant enzyme H46G
8.41
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.5, 37°C
8.63
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.5, 37°C
17
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme H46G
17
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme H46G
17
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant H46G
0.0287
4-nitrophenyl-alpha-L-fucoside
-
wild-type and mutant fucA1B
0.6
4-nitrophenyl-alpha-L-fucoside
wild-type enzyme, pH 8, 45°C
5.8
4-nitrophenyl-alpha-L-fucoside
mutant enzyme W392A, pH 8, 45°C
0.075
alpha-L-fucosyl fluoride
-
pH 5.0, 25°C, mutant E266A
0.15
alpha-L-fucosyl fluoride
-
pH 5.0, 25°C, wild-type enzyme
0.23
alpha-L-fucosyl fluoride
pH 5.0, 25°C
2.5
alpha-L-fucosyl fluoride
-
pH 5.0, 25°C, mutant E66A
0.11
p-nitrophenyl alpha-L-fucopyranoside
mutant E289Q, 50 mM sodium phosphate buffer, pH 6.8, 37°C, loss of catalytic activity
0.12
p-nitrophenyl alpha-L-fucopyranoside
mutant E289G, 50 mM sodium phosphate buffer, pH 6.8, 37°C, loss of catalytic activity
0.2
p-nitrophenyl alpha-L-fucopyranoside
-
pH 6.5, 37°C
0.27
p-nitrophenyl alpha-L-fucopyranoside
mutant D258G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.28
p-nitrophenyl alpha-L-fucopyranoside
50 mM sodium phosphate buffer, pH 6.8, 37°C
0.28
p-nitrophenyl alpha-L-fucopyranoside
mutant D158G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.28
p-nitrophenyl alpha-L-fucopyranoside
mutant D276G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.28
p-nitrophenyl alpha-L-fucopyranoside
mutant E135G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.28
p-nitrophenyl alpha-L-fucopyranoside
mutant E275G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.28
p-nitrophenyl alpha-L-fucopyranoside
mutant E70G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.3
p-nitrophenyl alpha-L-fucopyranoside
mutant E79G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.65
p-nitrophenyl alpha-L-fucopyranoside
-
pH 4, 60°C
1.04
p-nitrophenyl alpha-L-fucopyranoside
mutant D225G, 50 mM sodium phosphate buffer, pH 6.8, 37°C, loss of catalytic activity
1.06
p-nitrophenyl alpha-L-fucopyranoside
mutant D225N, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.028
p-nitrophenyl alpha-L-fucoside
-
pH 6.3, 65°C
0.032
p-nitrophenyl alpha-L-fucoside
pH 5.0, 60°C
0.034
p-nitrophenyl alpha-L-fucoside
pH 5.0, 37°C
0.05
p-nitrophenyl alpha-L-fucoside
-
pH 5.0, 37°C, wild-type enzyme
0.25
p-nitrophenyl alpha-L-fucoside
-
pH 6.8, 25°C
0.33
p-nitrophenyl alpha-L-fucoside
-
pH 6.8, 25°C, epididymis
0.75
p-nitrophenyl alpha-L-fucoside
-
pH 6.8, 25°C
0.75
p-nitrophenyl alpha-L-fucoside
-
pH 6.8, 25°C, kidney
2.1
p-nitrophenyl alpha-L-fucoside
-
pH 5.0, 37°C, mutant E66A
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.047
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride
pH 5.0, 60°C
1.01
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 5.0, 37°C
39.8 - 111
4-nitrophenyl alpha-L-fucopyranoside
0.48 - 198
4-nitrophenyl alpha-L-fucoside
0.04 - 846
4-nitrophenyl-alpha-L-fucopyranoside
137 - 287
4-nitrophenyl-alpha-L-fucoside
15.4
alpha-L-fucopyranosyl-(1->2)-D-galactopyranose
pH 7, 37°C
0.13 - 7.6
alpha-L-fucosyl fluoride
26.9
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc
pH 7, 37°C
23.6
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc
pH 7, 37°C
0.003 - 17.1
p-nitrophenyl alpha-L-fucopyranoside
0.24 - 287
p-nitrophenyl alpha-L-fucoside
39.8
4-nitrophenyl alpha-L-fucopyranoside
pH 5.5, 37°C
111
4-nitrophenyl alpha-L-fucopyranoside
pH 7.0, 37°C
0.48
4-nitrophenyl alpha-L-fucoside
mutant G226S/T264A/Y267F/L322P, at 40°C
0.5
4-nitrophenyl alpha-L-fucoside
mutant T264A/Y267F/L322P, at 40°C
0.73
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A/Y267F/L322P, at 40°C
2.7
4-nitrophenyl alpha-L-fucoside
mutant G226S/Y237H/T264A/L322P, at 40°C
3.6
4-nitrophenyl alpha-L-fucoside
mutant T264A/L322P, at 40°C
4.3
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A/Y267F, at 40°C
5.1
4-nitrophenyl alpha-L-fucoside
wild-type, at 40°C
6.4
4-nitrophenyl alpha-L-fucoside
mutant G226S/T264A/Y267F/L322P, at 70°C
8.2
4-nitrophenyl alpha-L-fucoside
mutant T264A/Y267F/L322P, at 70°C
14.8
4-nitrophenyl alpha-L-fucoside
mutant Y237H/Y267F/L322P, at 40°C
15
4-nitrophenyl alpha-L-fucoside
mutant R147G/L322P, mutant P196L/Y237H/Y267F and mutant T264A, at 40°C
20
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A, at 40°C
20
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A/Y267F/L322P, at 70°C
35
4-nitrophenyl alpha-L-fucoside
wild-type, at 70°C
39
4-nitrophenyl alpha-L-fucoside
mutant G226S/Y237H/T264A/L322P, at 70°C
50
4-nitrophenyl alpha-L-fucoside
mutant T264A/L322P7, at 70°C
56
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A/Y267F, at 70°C
65
4-nitrophenyl alpha-L-fucoside
mutant M210V/Y237H/T264A, at 70°C
96
4-nitrophenyl alpha-L-fucoside
mutant Y237H/Y267F/L322P, at 70°C
164
4-nitrophenyl alpha-L-fucoside
mutant P196L/Y237H/Y267F, at 70°C
169
4-nitrophenyl alpha-L-fucoside
mutant T264A, at 70°C
198
4-nitrophenyl alpha-L-fucoside
mutant R147G/L322P, at 70°C
0.04
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.5, 37°C
0.24
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D242G
0.24
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme D242G
0.48
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme G226S/T264A/Y267F/L322P
0.5
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A/Y267F/L322P
0.73
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A/Y267F/L322P
1.86
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E292G at pH 6.3 in the presence of sodium phosphate or at pH 4.6 in the presence of sodium acetate
1.86
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme E292G
1.96
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme E292G
2.7
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme G226S/Y237H/T264A/L322P
3.6
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A/L322P
3.61
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.5, 37°C
3.73
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.5, 37°C
4.3
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A/Y267F
5.1
4-nitrophenyl-alpha-L-fucopyranoside
40°C, wild-type enzyme
6.4
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme G226S/T264A/Y267F/L322P
8.2
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A/Y267F/L322P
9.66
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D242G, in the presence of 2 M sodium azide
14.8
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme Y237H/Y267F/L322P
15
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme P196L/Y237H/Y267F
15
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme R147G/L322P
15
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A
20
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A
20
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme M210V/Y237H/T264A/Y267F/L322P
35
4-nitrophenyl-alpha-L-fucopyranoside
70°C, wild-type enzyme
39
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme G226S/Y237H/T264A/L322P4
50
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A/L322P
56
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme M210V/Y237H/T264A/Y267F
65
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme M210V/Y237H/T264A
96
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme Y237H/Y267F/L322P
143
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium citrate
164
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme P196L/Y237H/Y267F
169
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A
198
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme R147G/L322P
224
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme D146G
224
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D146G
224
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme D146G
240
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme D124G
240
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D124G
240
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme D124G
287
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type enzyme
287
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, wild-type enzyme
287
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type, at pH 6.3, in the presence of sodium phosphate
419
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme E292G
419
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme H46G
419
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant enzyme H46G
419
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant H46G
430
4-nitrophenyl-alpha-L-fucopyranoside
-
pH5.0, 65°C, wild-type enzyme
430
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type, at pH 5.0
495
4-nitrophenyl-alpha-L-fucopyranoside
-
pH5.0, 65°C, mutant enzyme H46G
586
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium acetate
679
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 6.3, in the presence of sodium phosphate and NaN3
846
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium formate
137
4-nitrophenyl-alpha-L-fucoside
-
mutant fucA1B
287
4-nitrophenyl-alpha-L-fucoside
-
wild-type
0.13
alpha-L-fucosyl fluoride
-
pH 5.0, 25°C, mutant E266A
1.4
alpha-L-fucosyl fluoride
-
pH 5.0, 25°C, mutant E66A
2.9
alpha-L-fucosyl fluoride
pH 5.0, 25°C
7.6
alpha-L-fucosyl fluoride
-
pH 5.0, 25°C, wild-type enzyme
0.003
p-nitrophenyl alpha-L-fucopyranoside
mutant D225G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.003
p-nitrophenyl alpha-L-fucopyranoside
mutant D225N, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.039
p-nitrophenyl alpha-L-fucopyranoside
mutant E289Q, 50 mM sodium phosphate buffer, pH 6.8, 37°C
0.048
p-nitrophenyl alpha-L-fucopyranoside
mutant E289G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
12.5
p-nitrophenyl alpha-L-fucopyranoside
mutant D258G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
16
p-nitrophenyl alpha-L-fucopyranoside
mutant E135G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
16.3
p-nitrophenyl alpha-L-fucopyranoside
mutant E79G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
16.7
p-nitrophenyl alpha-L-fucopyranoside
mutant D276G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
16.9
p-nitrophenyl alpha-L-fucopyranoside
mutant D158G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
16.9
p-nitrophenyl alpha-L-fucopyranoside
mutant E275G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
16.9
p-nitrophenyl alpha-L-fucopyranoside
mutant E70G, 50 mM sodium phosphate buffer, pH 6.8, 37°C
17.1
p-nitrophenyl alpha-L-fucopyranoside
50 mM sodium phosphate buffer, pH 6.8, 37°C
0.24
p-nitrophenyl alpha-L-fucoside
-
pH 6.5, 65°C, D242G mutant
1.3
p-nitrophenyl alpha-L-fucoside
-
pH 5.0, 37°C, mutant E66A
5.4
p-nitrophenyl alpha-L-fucoside
pH 5.0, 37°C
9.1
p-nitrophenyl alpha-L-fucoside
pH 5.0, 60°C
14.3
p-nitrophenyl alpha-L-fucoside
-
pH 5.0, 37°C, wild-type enzyme
245
p-nitrophenyl alpha-L-fucoside
-
pH 6.5, 65°C, wild-type enzyme
287
p-nitrophenyl alpha-L-fucoside
-
pH 6.3, 65°C
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