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Information on EC 3.2.1.51 - alpha-L-fucosidase
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3.2.1.51 alpha-L-fucosidaseSpecify your search results
Word Map
- 3.2.1.51
- lysosomal
- hydrolases
- oligosaccharide
- glycosidases
- fucosidosis
- alpha-d-mannosidase
- beta-d-galactosidase
- fucose
-
fucosylated
- lectin
-
sialic
- beta-d-glucuronidase
- neuraminidase
-
n-acetyl-beta-d-glucosaminidase
- beta-n-acetylglucosaminidase
- beta-d-glucosidase
- glycoconjugates
- alpha-d-galactosidase
-
fucose-containing
-
4-methylumbelliferyl
- alpha-d-glucosidase
- alpha-l-fucose
- angiokeratomas
-
fucosyltransferase
- i-cell
-
transglycosylation
-
exoglycosidase
- mucolipidosis
- hexosaminidase
- diagnostics
- diffusum
-
spaniel
- beta-mannosidase
- analysis
- almond
-
corporis
- medicine
- synthesis
-
springer
- food industry
-
intralysosomal
- arylsulphatase
- alpha-gal
- beta-n-acetylhexosaminidase
- europaeus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
alpha-l-fucosidase, acid hydrolase, fuca1, serum alpha-l-fucosidase, serum afu, alpha-fuc, fuca2, alf1_wf, alpha-l-fucosidase i, alphafuc, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alpha-fuc
alpha-fucosidase
-
-
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alpha-L-fucosidase
alpha-L-fucosidase 2
alpha-L-fucosidase I
alpha-L-fucosidase iso2
Alpha-L-fucoside fucohydrolase
alpha-L-fucoside fucohydrolase 2
bfo_2737
Eo0918
Eo3066
Eo3812
FucA
Fuca1
Fuca2
FucFA
TfFuc1
Alpha-L-fucoside fucohydrolase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an alpha-L-fucoside + H2O = L-fucose + an alcohol
-
-
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an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme catalyses the transfer of L-fucose from donor structures such as alpha-L FucpOpNP and alpha-L-FucpF to various GlcpNac derivates and glCP, forming alpha-(1-3)linkages
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
alpha-L-fucoside fucohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9037-65-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-fucosyllactose + H2O
alpha-L-fucose + lactose
-
-
-
?
2-chloro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-chloro-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride + H2O
fluoride + 2-deoxy-2-fluoro-alpha-L-fucose
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-
-
?
2-fluoro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-fluoro-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-methyl-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-methyl-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-nitrophenyl alpha-L-fucopyranoside + H2O
2-nitrophenol + alpha-L-fucopyranose
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-
-
-
?
3-fucosyllactose + H2O
L-fucose + ?
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cleavage of alpha-1,3 bond
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-
?
4-methylumbelliferyl alpha-L-fucopyranoside
4-methylumbelliferone + alpha-L-fucose
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-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucopyranose
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl 2-sulfo-alpha-L-fucopyranoside + H2O
4-nitrophenol + 2-sulfo-alpha-L-fucopyranose
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hydrolysis at 1.9% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
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hydrolysis at 1.6% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
sodium acetate buffer 50 mM, pH 5.0, 37°C
-
-
?
4-nitrophenyl alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
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-
-
?
4-nitrophenyl-alpha-L-fucopyranoside
?
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assay at pH 4.5, 37°C for 3 h, reaction stopped by addition of borate buffer
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-
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-fucose-(1-3)-D-GlcNAc
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catalytic residues Asp242, Glu58, and Glu292
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-
?
4-nitrophenyl-alpha-L-fucoside + H2O
alpha-L-fucopyranose + 4-nitrophenol
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-
-
-
?
6-O-alpha-L-Fuc-N,N'-diacetylchitobiose + H2O
alpha-L-fucose + N,N'-diacetylchitobiose
-
-
-
?
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
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Fuc-alpha-1,6-GlcNAc-linkage most susceptible
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?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
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same yield of transglycosylation and hydrolysis products
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?
alpha-L-Fuc-(1-3)-alpha-L-Fuc-O-4-nitrophenyl ester + H2O
?
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-
-
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
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slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-3)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
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-
-
-
?
alpha-L-Fuc-(1-4)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
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-
-
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
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same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-6)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
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-
-
-
?
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe + H2O
alpha-L-fucose + beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe
-
-
-
?
alpha-L-Fuc-(1->4)-GlcNAc-poly-acrylamide polymer + H2O
alpha-L-fucose + GlcNAc-poly-acrylamide polymer
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-
-
?
alpha-L-fucopyranosyl fluoride + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
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13% yield based on the donor substrate
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?
alpha-L-fucopyranosyl fluoride + N-acetylglucosamine
2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranose + fluoride
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7% yield based on the donor substrate
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?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
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8% yield based on the donor substrate
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?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + fluoride
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34% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
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-
-
?
alpha-L-fucopyranosyl-(1->2)-D-galactopyranose + H2O
alpha-L-fucose + D-galactose
the enzyme does not hydrolyze other fucosyloligosaccharides or 4-nitrophenyl-alpha-L-fucoside
-
-
?
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GlcNAc
slightly hydrolyzed, since fucose monosaccharide is detected after the reaction
-
-
?
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1->3)-D-GlcNAc
i.e. Le(x) trisaccharide
-
-
?
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-OMe + H2O
?
-
-
-
?
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1->4)-D-GlcNAc
i.e. Le(a) trisaccharide
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
alpha-L-fucose + Fuc-beta-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
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lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
alpha-L-fucose + beta-galactosyl-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
alpha-L-fucose + GlcNAc-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
-
-
?
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
-
-
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
low activity
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
Bacillus fulminans
-
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues
-
?
glycopeptide from desialyzed porcine submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
-
?
glycosphingolipid + H2O
alpha-L-fucose + ?
-
blood-group active types B and H
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
lacto-N-difucohexaose I + H2O
L-fucose + ?
-
cleavage of alpha-1,4 bond
-
-
?
lacto-N-difucohexaose II + H2O
L-fucose + ?
-
cleavage of alpha-1,4 bond
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
m-nitrophenyl alpha-L-fucopyranoside + H2O
m-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
-
-
?
mono-sialylated-galactosylated biantennary N-glycan-core-substituted with alpha-(1->6)-linked fucose + H2O
L-fucose + ?
-
-
-
-
?
orosomucoid + H2O
alpha-L-fucose + ?
-
low activity, higher activity if desialyzed
-
?
p-cyanophenyl alpha-L-fucopyranoside + H2O
p-cyanophenol + L-fucose
pH 6.8, 37°C
-
-
?
p-nitrophenyl alpha-L-fucopyranoside + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
29% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
34% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + p-nitrophenol
-
-
25% yield based on the donor substrate
-
?
p-nitrophenyl-alpha-L-fucoside + H2O
L-fucose + p-nitrophenol
the enzyme has low transglycosylation properties
-
-
?
porcine submaxillary mucine, desialyzed + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
-
?
propyl alpha-L-fucopyranoside + H2O
propanol + alpha-L-fucopyranose
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside + H2O
?
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside + H2O
?