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Information on EC 3.2.1.51 - alpha-L-fucosidase
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3.2.1.51 alpha-L-fucosidaseSpecify your search results
Word Map
- 3.2.1.51
- lysosomal
- hydrolases
- glycosidases
- oligosaccharide
- fucosidosis
- alpha-d-mannosidase
- beta-d-galactosidase
-
fucosylated
- fucose
- neuraminidase
- beta-d-glucuronidase
-
n-acetyl-beta-d-glucosaminidase
- lectin
-
sialic
- beta-d-glucosidase
- glycoconjugates
- beta-n-acetylglucosaminidase
- alpha-d-galactosidase
-
transglycosylation
-
4-methylumbelliferyl
-
fucosyltransferase
- mucolipidosis
-
exoglycosidase
-
fucose-containing
- alpha-d-glucosidase
- alpha-l-fucose
- angiokeratomas
- i-cell
- hexosaminidase
- beta-n-acetylhexosaminidase
- alpha-gal
- almond
- europaeus
- food industry
-
intralysosomal
- synthesis
-
spaniel
- beta-mannosidase
- diagnostics
-
corporis
- diffusum
- medicine
- analysis
-
springer
- arylsulphatase
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
acid hydrolase, Afc2, Afc3, AFU, Alf1_Wf, alfA, alfB, alfC, alpha-fuc, alpha-fucosidase, 
more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
alfA
alfB
alfC
alpha-fuc
alpha-fucosidase
-
-
-
-
alpha-L-fucosidase
alpha-L-fucosidase 2
alpha-L-fucosidase I
alpha-L-fucosidase iso2
Alpha-L-fucoside fucohydrolase
alpha-L-fucoside fucohydrolase 2
bfo_2737
Eo0918
Eo3066
Eo3812
FucA
Fuca1
Fuca2
FucFA
TfFuc1
Alpha-L-fucoside fucohydrolase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an alpha-L-fucoside + H2O = L-fucose + an alcohol
-
-
-
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme catalyses the transfer of L-fucose from donor structures such as alpha-L FucpOpNP and alpha-L-FucpF to various GlcpNac derivates and glCP, forming alpha-(1-3)linkages
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
BRENDA
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SYSTEMATIC NAME
IUBMB Comments
alpha-L-fucoside fucohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
9037-65-4
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2'-fucosyllactose + H2O
alpha-L-fucose + lactose
-
-
-
?
2-chloro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-chloro-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride + H2O
fluoride + 2-deoxy-2-fluoro-alpha-L-fucose
-
-
-
?
2-fluoro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-fluoro-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
2-methyl-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-methyl-4-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
3-fucosyllactose + H2O
L-fucose + ?
-
cleavage of alpha-1,3 bond
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucopyranose
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl 2-sulfo-alpha-L-fucopyranoside + H2O
4-nitrophenol + 2-sulfo-alpha-L-fucopyranose
-
hydrolysis at 1.9% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
-
hydrolysis at 1.6% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
sodium acetate buffer 50 mM, pH 5.0, 37°C
-
-
?
4-nitrophenyl alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside
?
-
assay at pH 4.5, 37°C for 3 h, reaction stopped by addition of borate buffer
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-fucose-(1-3)-D-GlcNAc
-
catalytic residues Asp242, Glu58, and Glu292
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
alpha-L-fucopyranose + 4-nitrophenol
-
-
-
-
?
6-O-alpha-L-Fuc-N,N'-diacetylchitobiose + H2O
alpha-L-fucose + N,N'-diacetylchitobiose
-
-
-
?
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-alpha-L-Fuc-O-4-nitrophenyl ester + H2O
?
-
-
-
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-6)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe + H2O
alpha-L-fucose + beta-D-Gal-(1->4)-beta-D-GlcNAc-OMe
-
-
-
?
alpha-L-Fuc-(1->4)-GlcNAc-poly-acrylamide polymer + H2O
alpha-L-fucose + GlcNAc-poly-acrylamide polymer
-
-
-
?
alpha-L-fucopyranosyl fluoride + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
-
-
13% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + N-acetylglucosamine
2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranose + fluoride
-
-
7% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
-
-
8% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + fluoride
-
-
34% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
-
-
?
alpha-L-fucopyranosyl-(1->2)-D-galactopyranose + H2O
alpha-L-fucose + D-galactose
the enzyme does not hydrolyze other fucosyloligosaccharides or 4-nitrophenyl-alpha-L-fucoside
-
-
?
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GlcNAc
slightly hydrolyzed, since fucose monosaccharide is detected after the reaction
-
-
?
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1->3)-D-GlcNAc
i.e. Le(x) trisaccharide
-
-
?
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-beta-D-GlcNAc-OMe + H2O
?
-
-
-
?
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc + H2O
alpha-L-fucose + beta-D-Gal-(1->4)-D-GlcNAc
i.e. Le(a) trisaccharide
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
alpha-L-fucose + Fuc-beta-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
alpha-L-fucose + beta-galactosyl-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
alpha-L-fucose + GlcNAc-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
-
-
?
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
-
-
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
low activity
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
Bacillus fulminans
-
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues
-
?
glycopeptide from desialyzed porcine submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
-
?
glycosphingolipid + H2O
alpha-L-fucose + ?
-
blood-group active types B and H
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
lacto-N-difucohexaose I + H2O
L-fucose + ?
-
cleavage of alpha-1,4 bond
-
-
?
lacto-N-difucohexaose II + H2O
L-fucose + ?
-
cleavage of alpha-1,4 bond
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
m-nitrophenyl alpha-L-fucopyranoside + H2O
m-nitrophenol + L-fucose
pH 6.8, 37°C
-
-
?
Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
-
-
?
mono-sialylated-galactosylated biantennary N-glycan-core-substituted with alpha-(1->6)-linked fucose + H2O
L-fucose + ?
-
-
-
-
?
orosomucoid + H2O
alpha-L-fucose + ?
-
low activity, higher activity if desialyzed
-
?
p-cyanophenyl alpha-L-fucopyranoside + H2O
p-cyanophenol + L-fucose
pH 6.8, 37°C
-
-
?
p-nitrophenyl alpha-L-fucopyranoside + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
29% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
34% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + p-nitrophenol
-
-
25% yield based on the donor substrate
-
?
p-nitrophenyl-alpha-L-fucoside + H2O
L-fucose + p-nitrophenol
the enzyme has low transglycosylation properties
-
-
?
phenyl alpha-L-fucopyranoside + H2O
phenol + L-fucose
pH 6.8, 37°C
-
-
?
porcine submaxillary mucine, desialyzed + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
-
-
-
?
propyl alpha-L-fucopyranoside + H2O
propanol + alpha-L-fucopyranose
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->2)-alpha-L-fucopyranoside + H2O
?
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->3)-alpha-L-fucopyranoside + H2O
?
-
-
-
?
propyl alpha-L-fucopyranosyl-(1->4)-alpha-L-fucopyranoside + H2O
?
-
-
-
?
submaxilary mucin trisaccharide + H2O
alpha-L-fucose + disaccharide
-
-
-
?
Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside
-
-
-
?
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Fucalpha(1-2)Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + H2O
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + alpha-L-fucose
-
-
-
-
?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase II highly active
-
?
2-nitrophenyl alpha-L-fucopyranoside + H2O
2-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
2-nitrophenyl alpha-L-fucopyranoside + H2O
2-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
very low activity
-
?
3-fucosyllactose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond, low efficiency
-
-
?
3-fucosyllactose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond, low efficiency
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucopyranose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucopyranose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
induces acrosome reaction of guinea pig spermatozoa
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
responsible for the intralysosomal hydrolysis of fucose-containing glypeptide and glycolipids
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
involved in sperm-egg interactions
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
-
hydrolysis at 1.7% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-Fuc-(1->3)-beta-D-Gal-(1->4)-beta-D-Glc + H2O
alpha-L-fucose + 4-nitrophenyl beta-D-Gal-(1->4)-beta-D-Glc
-
hydrolysis at 3.7% compared to 4-nitrophenyl-alpha-L-fucopyranoside
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
the enzyme shows a low transglycosylation potential
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
Paenibacillus thiaminolyticus CCM 3599
the enzyme shows a low transglycosylation potential
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
the enzyme shows a low transglycosylation potential
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
WP_057784261.1
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucopyranose
Formosa algae KMM 3553T
WP_057784261.1
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
substrate incubated with plasma sample of breast cancer patients treated with anti-cancer monoclonal antibody trastuzumbal
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
-
transglycosylation activity
no other isomer
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
-
transglycosylation activity
no other isomer
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
-
?
alpha-L-Fuc-(1-3)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
alpha-L-Fuc-(1-3)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
alpha-L-Fuc-(1-4)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
alpha-L-Fuc-(1-4)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Bacillus fulminans
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
only hydrolysed by alpha-L-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Bacillus fulminans
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
only hydrolysed by alpha-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc, alpha-(1-3)-GlcNAc or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
NaBH4-reduced oligosaccharide, hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
-
fase I, not: fase II
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
-
fase I, not: fase II
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity for alpha-L-fucosidase II
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
NaBH4-reduced oligosaccharide, very slow hydrolysis
-
?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
very slow hydrolysis, NaBH4-reduced oligosaccharide
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
low activity
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
from meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillry mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillary mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
cellular source of substrate
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
not active on native glycoproteins
-
?
glycoprotein + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
-
?
glycoprotein + H2O
alpha-L-fucose + ?
-
salivary human type H
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
low activity for alpha-L-fucosidase II
-
?
lacto-N-fucopentaose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond
-
-
?
lacto-N-fucopentaose + H2O
L-fucose +
-
cleavage of alpha-1,3 bond, low efficiency
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
preferentially hydrolysed by alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
-
or methanol or ethanol as acceptor
-
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
-
or methanol or ethanol as acceptor
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
-
-
anomeric configuration of the produced fucopyranse
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
-
or methanol or ethanol as acceptor
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed and not, desialyzed mucine best substrate tested
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
one form, optimally active at pH 5, not able to hydrolyse p-nitrophenyl-alpha-L-fucopyranoside
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
hydrolysis only after pronase or neuraminidase treatment
-
?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
-
-
?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
subscript OT is used to indicate NaBH4-reduced oligosaccharide
-
?
additional information
?
-
-
series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis
-
-
?
additional information
?
-
-
series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
Afc2 shows enzyme activities exclusively to Le(x) trisaccharide and Le(a) trisaccharide. Although 3-fucosyl-GlcNAc and 4-fucosyl-GlcNAc have the same fucosyl linkage with Le(x) or Le(a) trisaccharides, they are resistant to the hydrolysis by Afc2 because they do not have the branched Gal residues at the non-reducing end. Afc2 is a 1,3-1,4-alphs-L-fucosidase
-
-
?
additional information
?
-
Afc2 shows enzyme activities exclusively to Le(x) trisaccharide and Le(a) trisaccharide. Although 3-fucosyl-GlcNAc and 4-fucosyl-GlcNAc have the same fucosyl linkage with Le(x) or Le(a) trisaccharides, they are resistant to the hydrolysis by Afc2 because they do not have the branched Gal residues at the non-reducing end. Afc2 is a 1,3-1,4-alphs-L-fucosidase
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency
-
-
?
additional information
?
-
-
the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively
-
-
?
additional information
?
-
-
the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides
-
-
?
additional information
?
-
-
the enzyme can only cleave alpha1,3-linked fucose residues from 3-fucosyllactose and lacto-N-fucopentaose III with low efficiency
-
-
?
additional information
?
-
-
the enzyme hydrolyzes alpha-(1->6)-linked fucose exclusively
-
-
?
additional information
?
-
-
the enzyme showed good hydrolytic activities towards various alpha1,3- and alpha1,4-bearing fucosyloligosaccharide glycan structures, whereas no defucosylation is observed with the tested alpha1,2- and alpha1,6-fucose containing oligosaccharides
-
-
?
additional information
?
-
the enzyme preferentially hydrolyzes the alpha-(1->4)-L-fucosidic linkage present in alpha-L-fucobiosides with different types of linkages. The enzyme is capable of catalyzing the transglycosylation reaction with alcohols (methanol, ethanol, and 1-propanol) and 4-nitrophenyl-containing monosaccharides as acceptors
-
-
?
additional information
?
-
-
the enzyme preferentially hydrolyzes the alpha-(1->4)-L-fucosidic linkage present in alpha-L-fucobiosides with different types of linkages. The enzyme is capable of catalyzing the transglycosylation reaction with alcohols (methanol, ethanol, and 1-propanol) and 4-nitrophenyl-containing monosaccharides as acceptors
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
no substrate: unable to hydrolyze 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl N-acetyl-beta-D-glucosaminide, lactose, melibiose, lactulose, maltose, or maltotriose
-
-
?
additional information
?
-
-
no substrate: unable to hydrolyze 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl N-acetyl-beta-D-glucosaminide, lactose, melibiose, lactulose, maltose, or maltotriose
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
the enzyme is capable of transglycosylation on a variety of acceptor molecules, including saccharides, alcohols or chromogenic substrates
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan
-
-
?
additional information
?
-
-
Two specific mutations, designed on the basis of the programmed -1 frameshifting mechanism, promotes transfucosylation reactions by following a retaining reaction mechanism.
-
-
?
additional information
?
-
-
4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-glucoside, 4-nitrophenyl-alpha-D-xyloside, 4-nitrophenyl-alpha-D-galactoside and 4-nitrophenyl-alpha-D-mannoside are not substrates for wild-type and mutant fucA1B
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
-
-
?
additional information
?
-
-
TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
-
-
?
additional information
?
-
TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
-
-
?
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
?
additional information
?
-
-
behaviour of the catalytic residues is different from that of Sulfolobus solfataricus alpha-fuc
-
-
?
additional information
?
-
-
the wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a 4-nitrophenyl-fucoside donor to 4-nitrophenyl-fucoside (self-condensation) with alpha-(1,3) regioselectivity or 4-nitrophenyl-galactoside (transglycosylation) with alpha-(1,2) regioselectivity at low yields (7%)
-
-
?
additional information
?
-
the wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a 4-nitrophenyl-fucoside donor to 4-nitrophenyl-fucoside (self-condensation) with alpha-(1,3) regioselectivity or 4-nitrophenyl-galactoside (transglycosylation) with alpha-(1,2) regioselectivity at low yields (7%)
-
-
?
additional information
?
-
-
in the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor, the presence of 1.1 M CaCl2 favors the rate of transfucosylation, and improves the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 mM and 146 mM, respectively. CaCl2 does not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in water activity and lactose concentration has a synergistic effect favoring the synthesis of fucosylated oligosaccharides
-
-
?
additional information
?
-
-
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
-
-
?
additional information
?
-
-
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
-
-
?
additional information
?
-
Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
-
-
?
additional information
?
-
Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
-
-
?
additional information
?
-
Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
-
-
?
additional information
?
-
Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
-
-
?
additional information
?
-
Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
-
-
?
additional information
?
-
Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
-
-
?
additional information
?
-
Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose
-
-
?
additional information
?
-
the enzyme can hydrolyze the partially degraded fragment of fucoidan. No free fucose can be released from alpha-Fuc-(1->2)-beta-Gal-(1->4)-Glc, beta-Gal-(1->4)-[alpha-Fuc-(1->3)]-Glc or alpha-Fuc-(1->6)-GlcNAc
-
-
?
additional information
?
-
-
the enzyme can hydrolyze the partially degraded fragment of fucoidan. No free fucose can be released from alpha-Fuc-(1->2)-beta-Gal-(1->4)-Glc, beta-Gal-(1->4)-[alpha-Fuc-(1->3)]-Glc or alpha-Fuc-(1->6)-GlcNAc
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-fucopyranosyl-(1->2)-beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol + H2O
alpha-L-fucose + beta-D-galactopyranosyl-(1->4)-2-acetamido-2-deoxy-D-galactitol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
-
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
alpha-L-fucose + Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc
-
low activity
-
?
Fuc-alpha-(1->2)-Gal-beta-(1->3)-[Fuc-alpha-(1->6)]GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O + H2O
alpha-L-fucose + Fuc-alpha-(1->2)-Gal-beta-(1->3)-GlcNAc-beta-(1->3)-Gal-beta-(1->4)GlcNAc + H2O
Bacillus fulminans
-
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
very slow hydrolysis, NaBH4-reduced oligosaccharide
-
?
glycosphingolipid + H2O
alpha-L-fucose + ?
-
blood-group active types B and H
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
orosomucoid + H2O
alpha-L-fucose + ?
-
low activity, higher activity if desialyzed
-
?
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-[Fuc-alpha-(1->6)]GlcNAc + H2O
[Man-alpha-(1->6)]-[Man-alpha-(1->3)]-Man-beta-(1->4)-GlcNAc-beta-(1->4)-GlcNAc + alpha-L-fucose
-
-
-
?
additional information
?
-
-
the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan
-
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase II highly active
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
very low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Bacillus fulminans
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
only hydrolysed by alpha-L-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Bacillus fulminans
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
only hydrolysed by alpha-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity for alpha-L-fucosidase II
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
low activity
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillary mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
cellular source of substrate
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
not active on native glycoproteins
-
?
glycoprotein + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
-
?
glycoprotein + H2O
alpha-L-fucose + ?
-
salivary human type H
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
low activity for alpha-L-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
preferentially hydrolysed by alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed and not, desialyzed mucine best substrate tested
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
one form, optimally active at pH 5, not able to hydrolyse p-nitrophenyl-alpha-L-fucopyranoside
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
hydrolysis only after pronase or neuraminidase treatment
-
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
CaCl2
-
the hydrolytic activity displays a maximum increase of 138% in the presence of 1.1 M NaCl. The hydrolytic activity is higher when using CaCl2 compared to NaCl. In the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor, the presence of 1.1 M CaCl2 favors the rate of transfucosylation, and improves the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 mM and 146 mM, respectively. CaCl2 does not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in water activity and lactose concentration has a synergistic effect favoring the synthesis of fucosylated oligosaccharides
Cu2+
Fe2+
Mg2+
Mn2+
NaCl
-
the hydrolytic activity displays a maximum increase of 67% in the presence of 0.8 M NaCl
Zn2+
additional information
additional information
the addition of calcium, magnesium, cobalt, manganese, nickel, and zinc at 10 mM concentrations does not increase enzymatic rates
additional information
-
the addition of calcium, magnesium, cobalt, manganese, nickel, and zinc at 10 mM concentrations does not increase enzymatic rates
additional information
the enzyme is not significantly influenced by NaCl
additional information
-
the enzyme is not significantly influenced by NaCl
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2S,3R,4S,5R)-2-(propan-2-yl)piperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(2S,3R,4S,5S)-2-methyl-5-(phenylamino)pyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-(1H-benzimidazol-2-yl)-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-[(biphenyl-4-ylamino)methyl]-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(5R,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 50% inhibition
(5R,6S,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 41% inhibition
(5S,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 49% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 55% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]-4-azaspiro[2.4]heptane
-
1 mM,97 inhibition, competitive
1,5-dideoxy-1,5-([(2R,3R)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2R,3S,4R,5R)-2,4,5,6-tetrahydroxy-3-(sulfooxy)hexyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-[5-methoxycarbonyl-1-pentyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1,5-dideoxy-1,5-[methyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
-
complete inactivation at 22 mM
2-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-(hydroxymethyl)-5-methylpyrrolidine-3,4-diol
-
competitive or uncompetitive inhibition, Ki: 0.004-26 mM, depending on conformation
2-cyclopentyl-2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)acetamide
-
2-cyclopentyl-2-phenyl-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)quinoline-4-carboxamide
-
2-phenyl-tetrahydro-2H-pyran-3,4-diol
-
uncompetitive inhibition, Ki: 22-83 mM, depending on conformation
3-(1H-indol-3-yl)-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)propanamide
-
3-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]propanamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
4-hydroxy-2-methyltetrahydro-2H-pyran-3-yl acetate
-
competitive inhibition, Ki: 0.09 mM
4-nitrophenyl alpha-L-fucopyranoside
substrate inhibition at high concentrations
5-fluoro-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)-1H-indole-2-carboxamide
-
L-fucal
-
competitive inhibition, best inhibitor of glycal compounds tested, Ki: 0.05 mM
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1-benzofuran-2-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1H-indole-3-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
-
p-nitrophenyl alpha-L-fucoside
substrate inhibition at high concentration
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
4-chloromercuribenzoate
-
50% inhibition at 0.0015 mM, totally reversal by addition of 4 mM DTT
Ag+
-
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
alpha-L-fucose
-
competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis
fuconojirimycin
majority of C1-substituted fuconojirimycins act as reversible inhibitors of the human fucosidase
fuconojirimycin
-
majority of C1-substituted fuconojirimycins are slow tight-binding inhibitors of the Thermotoga enzyme, Tyr64 and Tyr267 are critically involved in closely interacting with the aglycon of inhibitors
Hg2+
-
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
additional information
WP_057784261.1
the enzyme is inhibited by metabolites in the aqueous ethanol extract of Fucus evanescens
-
additional information
-
time-associated loss in the activity of alpha-fucosidase post infection. Sialic acid release is correlated with a gradual loss in activity of the enzyme
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
azide
increases the rate of substrate cleavage in depending of azide concentration
formate
increases the rate of substrate cleavage in depending of azide concentration
methanol
linear increase in enzyme-catalyzed cleavage of p-nitrophenyl alpha-L-fucoside and fucosyl fluoride
additional information
-
cell-bound enzyme activity increases sharply following permeabilization of intact sperm
-
additional information
-
the enzyme activity is increases with the ionic strength from 10 mM to 200 mM
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
6.01
alpha-L-fucopyranosyl-(1->2)-D-galactopyranose
pH 7, 37°C
3.1
beta-D-Gal-(1->3)-[alpha-L-Fuc-(1->4)]-D-GlcNAc
pH 7, 37°C
2.46
beta-D-Gal-(1->4)-[alpha-L-Fuc-(1->3)]-D-GlcNAc
pH 7, 37°C
0.33
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