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Enzyme Nomenclature
Information on EC 3.2.1.51 - alpha-L-fucosidase
for references in articles please use BRENDA:EC3.2.1.51
Word Map on EC 3.2.1.51
- 3.2.1.51
- lysosomal
- glycoprotein
- glycosidases
- fucosidosis
- oligosaccharide
- alpha-d-mannosidase
- beta-d-galactosidase
- fucose
-
sialic
- neuraminidase
-
fucosylated
- beta-d-glucuronidase
-
n-acetyl-beta-d-glucosaminidase
-
beta-n-acetylglucosaminidase
- beta-d-glucosidase
-
glycoconjugates
- alpha-d-galactosidase
-
exoglycosidase
-
fucosyltransferase
- hexosaminidase
- mucolipidosis
- alpha-l-fucose
-
fucose-containing
- alpha-d-glucosidase
- i-cell
-
4-methylumbelliferyl
- medicine
-
intralysosomal
- analysis
-
spaniel
- angiokeratomas
- europaeus
- arylsulphatase
- almond
- beta-mannosidase
- fabry
- diagnostics
- alpha-gal
- beta-n-acetylhexosaminidase
-
cyanuric
-
springer
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
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EC NUMBER 
COMMENTARY 
3.2.1.51
-
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RECOMMENDED NAME
GeneOntology No.
alpha-L-fucosidase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an alpha-L-fucoside + H2O = L-fucose + an alcohol
-
-
-
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme catalyses the transfer of L-fucose from donor structures such as alpha-L FucpOpNP and alpha-L-FucpF to various GlcpNac derivates and glCP, forming alpha-(1-3)linkages
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides
-
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
alpha-L-fucoside fucohydrolase
-
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CAS REGISTRY NUMBER
COMMENTARY
9037-65-4
-
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
putative alpha-L-fucosidase, precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
putative uncharacterized protein; CB15, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
probable glycosyl hydrolase; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes; putative uncharacterized protein; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
Microscilla sp.
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
desert and oasis flies studied, male and female, Neot Hakikar (oasis), Jordan Valley spring (wet), Kfar Adumim starved (arid), Jordan Valley autumn (arid)
-
-
alpha-L-fucosidase, putative; W83, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
SH1, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
Q97NA6
SwissProt
putative glycosyl hydrolase; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
putative glycosyl hydrolase; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
; comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
alpha-L-fucosidase family protein; ATCC35405, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
strain pv. Citri str.306, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
pv. campestris str. 8004, comparative and phylogenetic analysis of alpha-L-fucosidase genes
Q4UXD3
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
probable alpha-L-fucosidase; YCH46, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
putative alpha-L-fucosidase protein; NCTC 9343, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
putative alpha-L-fucosidase; NCTC 9343, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
putative exported alpha-L-fucosidase protein; NCTC 9343, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
putative exported hydrolase; NCTC 9343, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
YCH46, comparative and phylogenetic analysis of alpha-L-fucosidase genes
Q64QR8, Q64VK9, Q64XY9, Q650P6
SwissProt
VPI5482, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
probable glycosyl hydrolase; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
glycosyl hydrolase, putative alpha-L-fucosidase; KPA171202, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
KPA171202, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SWissProt
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
-
-
-
208705, 208707, 208715, 208716, 208719, 208721, 208723, 208724, 208727, 208735, 208736, 208737, 208738, 654935, 655418, 655733, 656821, 656888, 664629, 667006, 679052, 682040, 698301, 699333, 699825
-
-
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Microscilla sp.
MS119, putative fucosidase; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
Microscilla sp.
MS120, putative fucosidase; RE1, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
MS119, putative fucosidase; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
MS120, putative fucosidase; RE1, comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
precursor; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
clone ZZZ276 mRNA sequence; comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
comparative and phylogenetic analysis of alpha-L-fucosidase genes
SwissProt
alpha-L-fucosidase, putative; comparative and phylogenetic analysis of alpha-L-fucosidase genes
GenBank
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
-
enzyme is involved in gamete recognition. Recombinant enzyme interacts with alpha-L-fucose residues present on the micropyle of the Drosophila melanogaster eggshell, confirming that it is a good candidate as receptor involved in gamete interactions in fruit fly
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-chloro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-chloro-4-nitrophenol + L-fucose
-
pH 6.8, 37°C
-
-
?
2-deoxy-2-fluoro-alpha-L-fucosyl fluoride + H2O
fluoride + 2-deoxy-2-fluoro-alpha-L-fucose
-
-
-
-
2-fluoro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-fluoro-4-nitrophenol + L-fucose
-
pH 6.8, 37°C
-
-
?
2-methyl-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-methyl-4-nitrophenol + L-fucose
-
pH 6.8, 37°C
-
-
?
2-nitrophenyl alpha-L-fucopyranoside + H2O
2-nitrophenol + alpha-L-fucopyranose
B3W8U6;, B3WB08;, B3WBB5;
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
sodium acetate buffer 50 mM, pH 5.0, 37°C
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside
?
-
assay at pH 4.5, 37°C for 3 h, reaction stopped by addition of borate buffer
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-fucose-(1-3)-D-GlcNAc
-
catalytic residues Asp242, Glu58, and Glu292
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
4-nitrophenol + alpha-L-fucose
-
activity measured in whole fly homogenates, substrate concentration of 6 mM in citrate buffer
-
-
?
4-nitrophenyl-alpha-L-fucoside + H2O
alpha-L-fucopyranose + 4-nitrophenol
-
-
-
-
?
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-alpha-L-Fuc-O-4-nitrophenyl ester + H2O
?
-
-
-
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-3)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
B3W8U6;, B3WB08;, B3WBB5;
-
-
-
?
alpha-L-Fuc-(1-4)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
B3W8U6;, B3WB08;, B3WBB5;
-
-
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-6)-D-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
B3W8U6;, B3WB08;, B3WBB5;
-
-
-
?
alpha-L-fucopyranosyl fluoride + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
-
-
13% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + N-acetylglucosamine
2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranose + fluoride
-
-
7% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + fluoride
-
-
8% yield based on the donor substrate
-
?
alpha-L-fucopyranosyl fluoride + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + fluoride
-
-
34% yield based on the donor substrate
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
alpha-L-fucose + Fuc-beta-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
alpha-L-fucose + beta-galactosyl-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
-
-
-
?
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
alpha-L-fucose + GlcNAc-4-nitrophenol
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
-
-
?
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
-
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues
-
?
glycosphingolipid + H2O
alpha-L-fucose + ?
Venus mercenaria
-
blood-group active types B and H
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
m-nitrophenyl alpha-L-fucopyranoside + H2O
m-nitrophenol + L-fucose
-
pH 6.8, 37°C
-
-
?
Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside
-
-
-
?
orosomucoid + H2O
alpha-L-fucose + ?
-
low activity, higher activity if desialyzed
-
?
p-cyanophenyl alpha-L-fucopyranoside + H2O
p-cyanophenol + L-fucose
-
pH 6.8, 37°C
-
-
?
p-nitrophenyl alpha-L-fucopyranoside + methyl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
methyl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
29% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-alpha-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-alpha-D-glucopyranoside + p-nitrophenol
-
-
34% yield based on the donor substrate
-
?
p-nitrophenyl alpha-L-fucopyranoside + prop-2-en-1-yl 2-(acetylamino)-2-deoxy-beta-D-glucopyranoside
prop-2-en-1-yl 2-(acetylamino)-2-deoxy-3-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-glucopyranoside + p-nitrophenol
-
-
25% yield based on the donor substrate
-
?
p-nitrophenyl-alpha-L-fucoside + H2O
L-fucose + p-nitrophenol
the enzyme has low transglycosylation properties
-
-
?
submaxilary mucin trisaccharide + H2O
alpha-L-fucose + disaccharide
-
-
-
?
submaxillary porcine trisaccharide + H2O
alpha-L-fucose + disaccharide
-
-
-
?
Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O
alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside
-
-
-
?
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Fucalpha(1-2)Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + H2O
Xylalpha(1-6)Glcbeta(1-4)[Xylalpha(1-6)]Glcbeta(1-4)[Galbeta(1-2)Xylalpha(1-6)]Glcbeta(1-4)Glc + alpha-L-fucose
-
-
-
-
?
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT-[Fuc-alpha-(1-6)] + H2O
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT + alpha-L-fucose
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
B3W8U6;, B3WB08;, B3WBB5;
-
-
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase II highly active
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
very low activity
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
induces acrosome reaction of guinea pig spermatozoa
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
responsible for the intralysosomal hydrolysis of fucose-containing glypeptide and glycolipids
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
involved in sperm-egg interactions
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
-
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
Venus mercenaria
-
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
-
substrate incubated with plasma sample of breast cancer patients treated with anti-cancer monoclonal antibody trastuzumbal
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
-
transglycosylation activity
no other isomer
?
4-nitrophenyl-alpha-L-fucopyranoside + N-acetyl-D-glucosamine + H2O
4-nitrophenol + alpha-L-Fuc-(1-3)-D-GlcNAc
-
transglycosylation activity
no other isomer
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
-
?
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
-
?
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Bacillus fulminans
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Venus mercenaria
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Venus mercenaria
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
only hydrolysed by alpha-L-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Bacillus fulminans
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
only hydrolysed by alpha-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc, alpha-(1-3)-GlcNAc or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
NaBH4-reduced oligosaccharide, hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
-
fase I, not: fase II
-
-
?
fucose residues from fucose-containing oligosaccharide chains on porcine gastric mucin + H2O
?
-
fase I, not: fase II
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity for alpha-L-fucosidase II
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
NaBH4-reduced oligosaccharide, very slow hydrolysis
-
?
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
very slow hydrolysis, NaBH4-reduced oligosaccharide
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
low activity
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
from meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillry mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillary mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
cellular source of substrate
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
not active on native glycoproteins
-
?
glycoprotein + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
low activity for alpha-L-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
preferentially hydrolysed by alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Venus mercenaria
-
-
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
-
or methanol or ethanol as acceptor
-
-
?
methyl alpha-L-fucopyranoside + H2O
methanol + alpha-L-fucose
-
or methanol or ethanol as acceptor
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharides + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
-
-
anomeric configuration of the produced fucopyranse
-
?
p-nitrophenyl alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucopyranose
-
or methanol or ethanol as acceptor
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
-
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
fase II, not: fase I
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
-
-
-
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed and not, desialyzed mucine best substrate tested
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
one form, optimally active at pH 5, not able to hydrolyse p-nitrophenyl-alpha-L-fucopyranoside
-
-
-
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
-
-
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
hydrolysis only after pronase or neuraminidase treatment
-
?
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT-[Fuc-alpha-(1-6)] + H2O
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT + alpha-L-fucose
-
-
-
?
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT-[Fuc-alpha-(1-6)] + H2O
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT + alpha-L-fucose
-
subscript OT is used to indicate NaBH4-reduced oligosaccharide
-
?
additional information
?
-
-
series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis
-
-
-
additional information
?
-
-
series of snapshots of the reaction coordinate. The Michaelis complex is observed in a 1C4 conformation, and a trapped covalent intermediate in the 3S1 skew boat which together provides structural evidence for a latitudinal Southern Hemisphere 1C4-3H4-3S1 pathway for terminal alpha-L-fucoside hydrolysis
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
B3W8U6;
no substrate: unable to hydrolyze 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl N-acetyl-beta-D-glucosaminide, lactose, melibiose, lactulose, maltose, or maltotriose
-
-
-
additional information
?
-
B3WB08;
no substrate: unable to hydrolyze 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl N-acetyl-beta-D-glucosaminide, lactose, melibiose, lactulose, maltose, or maltotriose
-
-
-
additional information
?
-
B3WBB5;
no substrate: unable to hydrolyze 4-nitrophenyl alpha-D-galactopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 2-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-glucopyranoside, 4-nitrophenyl beta-D-glucopyranoside, and 4-nitrophenyl N-acetyl-beta-D-glucosaminide, lactose, melibiose, lactulose, maltose, or maltotriose
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan
-
-
-
additional information
?
-
-
Two specific mutations, designed on the basis of the programmed -1 frameshifting mechanism, promotes transfucosylation reactions by following a retaining reaction mechanism.
-
-
-
additional information
?
-
-
4-nitrophenyl-alpha-L-arabinoside, 4-nitrophenyl-alpha-L-rhamnoside, 4-nitrophenyl-alpha-D-glucoside, 4-nitrophenyl-alpha-D-xyloside, 4-nitrophenyl-alpha-D-galactoside and 4-nitrophenyl-alpha-D-mannoside are not substrates for wild-type and mutant fucA1B
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
all eukaryotic alpha-L-fucosidases contain a catalytic N-terminal domain with a (beta/alpha)8-TIM barrel structure and a C-terminal domain
-
-
-
additional information
?
-
-
behaviour of the catalytic residues is different from that of Sulfolobus solfataricus alpha-fuc
-
-
-
additional information
?
-
the wild-type enzyme catalyzes oligosaccharide synthesis by transfer of a fucosyl residue from a 4-nitrophenyl-fucoside donor to 4-nitrophenyl-fucoside (self-condensation) with alpha-(1,3) regioselectivity or 4-nitrophenyl-galactoside (transglycosylation) with alpha-(1,2) regioselectivity at low yields (7%)
-
-
-
additional information
?
-
-
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
-
-
-
additional information
?
-
-
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
-
-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
6-O-alpha-L-fucopyranosyl-N-acetylglucosamine + H2O
alpha-L-fucopyranose + N-acetyl-glucosamine
-
Fuc-alpha-1,6-GlcNAc-linkage most susceptible
-
?
alpha-L-Fuc-(1-2)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
alpha-L-Fuc-(1-3)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-3)-D-GlcNAc + D-galactose
-
slower hydrolysis than for the alpha-(1-6)- or alpha-(1-2)-isomers, transglycosylation activity
-
?
alpha-L-Fuc-(1-6)-D-Gal + N-acetyl-D-glucosamine
alpha-L-Fuc-(1-6)-D-GlcNAc + D-galactose
-
same yield of transglycosylation and hydrolysis products
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
Fuc-alpha-(1-2)-Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-[Fuc-alpha-(1-4)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-difucohexaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-sorbitol + H2O
alpha-L-fucose + Gal-beta-(1-4)-sorbitol
-
alpha-fucosidase II, does not cleave alpha-(1-4)-GlcNAc-, alpha-(1-3)-GlcNAc- or alpha-(1-6)-GlcNAc-linkages and does not act on lacto-N-fucopentaoitol I, which has a Fuc-alpha-(1-2)-Gal-group
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
Gal-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + alpha-L-fucose
-
hydrolysis of Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-4)-GlcNAc- and Fuc-alpha-(1-6)-GlcNAc- linkages but hardly the Fuc-alpha-(1-3)-GlcNAc-linkage
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
-
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Gal-beta-(1-4)[Fuc-alpha-(1-3)]-Glc + H2O
Gal-beta-(1-4)-Glc + alpha-L-fucose
-
very slow hydrolysis, NaBH4-reduced oligosaccharide
-
?
glycosphingolipid + H2O
alpha-L-fucose + ?
Venus mercenaria
-
blood-group active types B and H
-
?
gp120 oligosaccharides + H2O
alpha-L-fucose + ?
-
released from recombinant CHO cell-produced gp120, glycoproteins containing fucose in alpha-1,3- and alpha-1,6-linkages are no substrates
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
orosomucoid + H2O
alpha-L-fucose + ?
-
low activity, higher activity if desialyzed
-
?
submaxillary porcine trisaccharide + H2O
alpha-L-fucose + disaccharide
-
-
-
?
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT-[Fuc-alpha-(1-6)] + H2O
[Man-beta-(1-6)-Man-alpha-(1-3)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAcOT + alpha-L-fucose
-
-
-
?
additional information
?
-
-
the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan
-
-
-
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
low activity
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase I, cleavage of alpha-(1-3)- and alpha-(1-4)-linkages in oligosaccharides and glycopeptides, doesn't cleave Fuc-alpha-(1-2)-Gal- and Fuc-alpha-(1-6)-GlcNAc-linkages
-
?
2'-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
alpha-fucosidase II highly active
-
?
3-fucosyllactitol + H2O
alpha-L-fucose + lactitol
-
very low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Bacillus fulminans
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Venus mercenaria
-
low activity
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Venus mercenaria
-
lacto-N-fucopentaose I
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
only hydrolysed by alpha-L-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + H2O
Gal-beta-(1-3)-GlcNAc-beta-(1-4)-Glc + alpha-L-fucose
-
lacto-N-fucopentaitol I, NaBH4-reduced oligosaccharide
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
Bacillus fulminans
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
only hydrolysed by alpha-fucosidase II
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
-
2'-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
low activity
-
?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-D-fucose + lactose
-
3-fucosyllactose
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
slow hydrolysis rate
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity for alpha-L-fucosidase II
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-3)-GlcNAc-beta-(1-3)-[Fuc-alpha-(1-4)]-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaitol II, NaBH4-reduced oligosaccharide
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
lacto-N-fucopentaose III
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
-
low activity
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed porcine submaxillary mucin
-
?
glycopeptide + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
meconium H-1, H-2 and X-1
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
cellular source of substrate
-
?
glycopeptide + H2O
alpha-L-fucose + ?
-
not active on native glycoproteins
-
?
glycoprotein + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
specifically hydrolyses terminal alpha-(1-2)-fucosidic bonds of glycoproteins, glycopeptides and oligosaccharides
-
?
lacto-N-fucopentaitol III + H2O
alpha-L-fucose + ?
-
low activity for alpha-L-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
preferentially hydrolysed by alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
alpha-fucosidase II
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
-
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
Venus mercenaria
-
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
-
?
oligosaccharide + H2O
alpha-L-fucose + ?
-
di-, tri- and pentasaacharides having alpha-1-2, alpha-1-3, and alpha-1-4 bonds
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
Bacillus fulminans
-
desialyzed and not, desialyzed mucine best substrate tested
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
submaxillary mucin + H2O
alpha-L-fucose + ?
-
one form, optimally active at pH 5, not able to hydrolyse p-nitrophenyl-alpha-L-fucopyranoside
-
-
-
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
-
-
submaxillary mucin + H2O
alpha-L-fucose + ?
-
A-minus type and desialyzed, porcine
-
?
thyroglobulin + H2O
alpha-L-fucose + ?
-
hydrolysis only after pronase or neuraminidase treatment
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
Fe2+
Zn2+
additional information
-
the addition of calcium, magnesium, cobalt, manganese, nickel, and zinc at 10 mM concentrations does not increase enzymatic rates
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(2S,3R,4S,5R)-2-(propan-2-yl)piperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(2S,3R,4S,5S)-2-methyl-5-(phenylamino)pyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-(1H-benzimidazol-2-yl)-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(2S,3S,4R,5S)-2-[(biphenyl-4-ylamino)methyl]-5-methylpyrrolidine-3,4-diol
-
inhibitor shares the same absolute configuration as C(2,3,4,5) of alpha-L-fucopyranosides
(5R,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 50% inhibition
(5R,6S,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 41% inhibition
(5S,6R,7R)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 49% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-hydroxymethyl-4-azaspiro[2.4]heptane
-
1 mM, 55% inhibition
(5S,6R,7S)-6,7-dihydroxy-5-[(1S)-1,2-dihydroxyethyl]-4-azaspiro[2.4]heptane
-
1 mM,97 inhibition, competitive
1,5-dideoxy-1,5-([(2R,3R)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2R,3S,4R,5R)-2,4,5,6-tetrahydroxy-3-(sulfooxy)hexyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-[5-methoxycarbonyl-1-pentyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1,5-dideoxy-1,5-[methyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1-cyclohexyl-3-(2-morpholinoethyl)-carbodiimide metho-p-toluenesulfonate
-
complete inactivation at 22 mM
2-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-(hydroxymethyl)-5-methylpyrrolidine-3,4-diol
-
competitive or uncompetitive inhibition, Ki: 0.004-26 mM, depending on conformation
2-cyclopentyl-2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)acetamide
-
-
2-cyclopentyl-2-phenyl-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)quinoline-4-carboxamide
-
-
2-phenyl-tetrahydro-2H-pyran-3,4-diol
-
uncompetitive inhibition, Ki: 22-83 mM, depending on conformation
3-(1H-indol-3-yl)-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)propanamide
-
-
3-(1H-indol-3-yl)-N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]propanamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
4-hydroxy-2-methyltetrahydro-2H-pyran-3-yl acetate
-
competitive inhibition, Ki: 0.09 mM
5-fluoro-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)-1H-indole-2-carboxamide
-
-
L-fucal
-
competitive inhibition, best inhibitor of glycal compounds tested, Ki: 0.05 mM
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1-benzofuran-2-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]-1H-indole-3-carboxamide
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
N-[[(2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl]methyl]acetamide
-
-
p-nitrophenyl alpha-L-fucoside
-
; substrate inhibition at high concentration
additional information
-
time-associated loss in the activity of alpha-fucosidase post infection. Sialic acid release is correlated with a gradual loss in activity of the enzyme
-
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
-
fuconojirimycin derivative, time dependent, slow-binding inhibition
4-chloromercuribenzoate
-
50% inhibition at 0.0015 mM, totally reversal by addition of 4 mM DTT
Ag+
-
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
alpha-L-fucose
-
competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis
fuconojirimycin
-
majority of C1-substituted fuconojirimycins act as reversible inhibitors of the human fucosidase
fuconojirimycin
-
majority of C1-substituted fuconojirimycins are slow tight-binding inhibitors of the Thermotoga enzyme, Tyr64 and Tyr267 are critically involved in closely interacting with the aglycon of inhibitors
Hg2+
-
complete inactivation at 0.1 mM, complete reversal by addition of 1.1 mM DTT
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
azide
-
increases the rate of substrate cleavage in depending of azide concentration
formate
-
increases the rate of substrate cleavage in depending of azide concentration
methanol
-
linear increase in enzyme-catalyzed cleavage of p-nitrophenyl alpha-L-fucoside and fucosyl fluoride
additional information
-
cell-bound enzyme activity increases sharply following permeabilization of intact sperm
-
additional information
-
the enzyme activity is increases with the ionic strength from 10 mM to 200 mM
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.27
2-nitrophenyl alpha-L-fucopyranoside
B3W8U6;, B3WB08;, B3WBB5;
pH 7.0, 37°C
2.9
2-nitrophenyl alpha-L-fucopyranoside
B3W8U6;, B3WB08;, B3WBB5;
pH 7.0, 37°C
5.2
2-nitrophenyl alpha-L-fucopyranoside
B3W8U6;, B3WB08;, B3WBB5;
pH 7.0, 37°C
0.06
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 5.0, 37°C, undifferentiated HT-29 cells
0.07
4-methylumbelliferyl alpha-L-fucopyranoside
-
pH 5.0, 37°C, differentiated HT-29 cells
0.14
4-methylumbelliferyl-alpha-L-fucopyranoside
-
4-5fold increase in fucosidosis patients
0.028
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, wild-type enzyme
0.028
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type, at pH 6.3, in the presence of sodium phosphate; wild-type enzyme
0.033
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme D146G
0.033
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D146G; mutant enzyme D146G
0.056
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme E292G
0.06
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A/Y267F/L322P; 70°C, mutant enzyme M210V/Y237H/T264A
0.06
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E292G, at pH 6.3, in the presence of sodium phosphate; mutant enzyme E292G
0.065
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A
0.07
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A
0.079
4-nitrophenyl-alpha-L-fucopyranoside
70°C, wild-type enzyme
0.08
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme G226S/Y237H/T264A/L322P
0.088
4-nitrophenyl-alpha-L-fucopyranoside
-
pH 6.3, 65°C, mutant enzyme D124G
0.09
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme G226S/T264A/Y267F/L322P; 40°C, mutant enzyme M210V/Y237H/T264A/Y267F; 40°C, mutant enzyme P196L/Y237H/Y267F
0.09
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant D124G; mutant E292G, at pH 4.6, in the presence of sodium acetate; mutant enzyme D124G
0.1
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A; 70°C, mutant enzyme G226S/Y237H/T264A/L322P4; 70°C, mutant enzyme P196L/Y237H/Y267F
0.13
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme R147G/L322P
0.14
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme M210V/Y237H/T264A/Y267F/L322P; 70°C, mutant enzyme M210V/Y237H/T264A/Y267F
0.19
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme T264A/L322P
0.23
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme Y237H/Y267F/L322P
0.25
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme M210V/Y237H/T264A/Y267F/L322P; 70°C, mutant enzyme T264A/L322P
0.26
4-nitrophenyl-alpha-L-fucopyranoside
-
pH5.0, 65°C, wild-type enzyme
0.26
4-nitrophenyl-alpha-L-fucopyranoside
-
wild-type, at pH 5.0, in the presence of sodium citrate
0.28
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme R147G/L322P
0.29
4-nitrophenyl-alpha-L-fucopyranoside
40°C, mutant enzyme Y237H/Y267F/L322P
0.3
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme G226S/T264A/Y267F/L322P
0.31
4-nitrophenyl-alpha-L-fucopyranoside
70°C, mutant enzyme T264A/Y267F/L322P
0.6
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium acetate
1.1
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium formate
1.6
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 4.6, in the presence of sodium citrate
2.9
4-nitrophenyl-alpha-L-fucopyranoside
-
mutant E58G, at pH 6.3, in the presence of sodium phosphate and NaN3
4.37
4-nitrophenyl-alpha-L-fucopyranoside
-
pH5.0, 65°C, mutant enzyme H46G