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Literature summary for 3.2.1.51 extracted from

  • Opheim, D.J.; Touster, O.
    The purification and characterization of rat liver lysosomal alpha-L-fucosidase (1977), J. Biol. Chem., 252, 739-743.
    View publication on PubMed

General Stability

General Stability Organism
stabilizing effect by addition of 0.05 mM/ml bovine serum albumin, 1 mM 2-mercaptoethanol, 1 mM MgSO4, high protein concentrations required Rattus sp.

Inhibitors

Inhibitors Comment Organism Structure
alpha-L-fucose competitive inhibition, Ki: 1.6 mM Rattus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.19
-
4-nitrophenyl-alpha-L-fucopyranoside
-
Rattus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
lysosome
-
Rattus sp. 5764
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
4 * 55000, SDS-PAGE Rattus sp.
217000
-
sedimentation equilibrium centrifugation Rattus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glycopeptide + H2O Rattus sp. not active on native glycoproteins alpha-L-fucose + ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus sp.
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein residues per tetramer: fucose 3.5, mannose 32, galactose 8: glucose 9, glucosamine 32, sialic acid 8 Rattus sp.

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, affinity chromatography Rattus sp.

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
27 28
-
Rattus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-fucopyranoside + H2O can cleave alpha-(1-2)-, alpha-(1-3)- and alpha-(1-4)- fucosyl linkages Rattus sp. 4-nitrophenol + alpha-L-fucose
-
?
glycopeptide + H2O not active on native glycoproteins Rattus sp. alpha-L-fucose + ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 55000, SDS-PAGE Rattus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.7 5.9
-
Rattus sp.