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Literature summary for 3.2.1.51 extracted from
- Purification and some properties of a beta-xylosidase and an alpha-fucosidase from apple snails (Pomacea canaliculata) (1996), Biosci. Biotechnol. Biochem., 60, 249-254.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-chloromercuribenzoate | 71% inhibition at 1 mM | Pomacea canaliculata |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54000 | - |
4 * 54000, SDS-PAGE | Pomacea canaliculata |
| 230000 | - |
sedimentation equilibrium centrifugation | Pomacea canaliculata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pomacea canaliculata | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Pomacea canaliculata |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| to homogeneity, chromatography steps | Pomacea canaliculata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| viscus | - |
Pomacea canaliculata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Pomacea canaliculata | 4-nitrophenol + alpha-L-fucose | - |
? | |
| GlcNAc-beta-(1-2)-Man-alpha-(1-6)-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O | not completely hydrolysed due to steric hinderance by beta-(1-2)-GlcNAc residues | Pomacea canaliculata | alpha-L-fucose + [GlcNAc-beta-(1-2)-Man-alpha-(1-6)]-[GlcNAc-beta-(1-2)-Man-alpha-(1-3)]-[Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside | - |
? | |
| Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O | - |
Pomacea canaliculata | alpha-L-fucose + Man-beta-(1-6)-[Man-alpha-(1-3)-Xyl-beta-(1-2)]-Man-beta-(1-4)-GlcNAc-beta-(1-4)-GlcNAc-pyridylaminoside | - |
? | |
| Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-(1-4)-[Fuc-alpha-(1-3)]-GlcNAc-pyridylaminoside + H2O | - |
Pomacea canaliculata | alpha-L-fucose + Xyl-beta-(1-2)-Man-beta-(1-4)-GlcNAc-beta-pyridylaminoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 54000, SDS-PAGE | Pomacea canaliculata |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
fairly stable up to | Pomacea canaliculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 3.2 | 5.2 | - |
Pomacea canaliculata |
| 5.7 | - |
- |
Pomacea canaliculata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | fairly stable within | Pomacea canaliculata |
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