Activating Compound | Comment | Organism | Structure |
---|---|---|---|
bovine serum albumin | increases acitivity up to 100% | Macaca radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-chloromercuribenzoate | 50% inhibition at 0.0015 mM, totally reversal by addition of 4 mM DTT | Macaca radiata | |
Ag2+ | complete inactivation at 1 mM | Macaca radiata | |
alpha-L-fucose | competitive inhibition, Ki: 0.275 mM | Macaca radiata | |
Cu2+ | complete inactivation at 1 mM | Macaca radiata | |
ethanolamine | strong inhibition | Macaca radiata | |
Hg2+ | complete inhibition at 1 mM | Macaca radiata | |
iodoacetamide | 30% inhibition at 1 mM | Macaca radiata | |
N-ethylmaleimide | 30% inhibition at 1 mM | Macaca radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
4-nitrophenyl-alpha-L-fucopyranoside | - |
Macaca radiata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73500 | - |
4 * 73500, SDS-PAGE, monomers and dimers also active | Macaca radiata |
285000 | - |
gel filtration | Macaca radiata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Macaca radiata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
to homogeneity, chromatography steps | Macaca radiata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Macaca radiata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl-alpha-L-fucopyranoside + H2O | - |
Macaca radiata | 4-nitrophenol + alpha-L-fucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 73500, SDS-PAGE, monomers and dimers also active | Macaca radiata |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 55 | triphasic heat inactivation due to 3 different heat sensitive forms, forms I and II: less heat sensitive, form III: most heat sensitive | Macaca radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
slight different pH-optima for monomeric, dimeric and tetrameric forms | Macaca radiata |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
instability below | Macaca radiata |