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Literature summary for 3.2.1.51 extracted from

  • Guzman-Rodriguez, F.; Alatorre-Santamaria, S.; Gomez-Ruiz, L.; Rodriguez-Serrano, G.; Garcia-Garibay, M.; Cruz-Guerrero, A.
    Improvement of the transfucosylation activity of alpha-L-fucosidase from Thermotoga maritima for the synthesis of fucosylated oligosaccharides in the presence of calcium and sodium (2018), Extremophiles, 22, 889-894 .
    View publication on PubMed
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Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 the hydrolytic activity displays a maximum increase of 138% in the presence of 1.1 M NaCl. The hydrolytic activity is higher when using CaCl2 compared to NaCl. In the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor, the presence of 1.1 M CaCl2 favors the rate of transfucosylation, and improves the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 mM and 146 mM, respectively. CaCl2 does not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in water activity and lactose concentration has a synergistic effect favoring the synthesis of fucosylated oligosaccharides Thermotoga maritima
NaCl the hydrolytic activity displays a maximum increase of 67% in the presence of 0.8 M NaCl Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl-alpha-L-fucoside + H2O
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 Thermotoga maritima 4-nitrophenol + alpha-L-fucose
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additional information in the synthesis of fucosylated oligosaccharides using 4-nitrophenyl-fucose as donor substrate and lactose as acceptor, the presence of 1.1 M CaCl2 favors the rate of transfucosylation, and improves the yield of synthesis duplicating and triplicating it with lactose concentrations of 58 mM and 146 mM, respectively. CaCl2 does not significatively affect hydrolysis rate in these reactions. The combination of the activating effect of CaCl2, the decrement in water activity and lactose concentration has a synergistic effect favoring the synthesis of fucosylated oligosaccharides Thermotoga maritima ?
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 ?