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Literature summary for 3.2.1.51 extracted from

  • Tsuji, Y.; Yamamoto, K.; Tochikura, T.; Seno, T.; Ohkubo, Y.; Yamaguchi, H.
    Purification and characterization of alpha-L-fucosidase from Bacillus circulans grown on porcine gastric mucin (1990), J. Biochem., 107, 324-330.
    View publication on PubMed
Search for more literature for 3.2.1.51

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ potent inhibitor at 1 mM Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.63
-
 2'-fucosyllactose
-
 Niallia circulans
0.88
-
 gastric mucin
-
 Niallia circulans
1.25
-
 3'-fucosyllactose
-
 Niallia circulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular maximal enzyme synthesis by growth on porcine gastric mucin containing media Niallia circulans
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
135000
-
 2 * 135000, SDS-PAGE Niallia circulans
285000
-
 gel filtration Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Niallia circulans lacto-N-fucopentaose I alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O Niallia circulans 2'-fucosyllactose alpha-L-fucose + lactose
-
 ?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O Niallia circulans 3-fucosyllactose alpha-D-fucose + lactose
-
 ?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O Niallia circulans lacto-N-fucopentaose III alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
gastric mucin + H2O Niallia circulans porcine alpha-L-fucose + ?
-
 ?
lacto-N-fucopentaose II + H2O Niallia circulans
-
 alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
lactoferrin + H2O Niallia circulans low activity alpha-L-fucose + ?
-
 ?
milk + H2O Niallia circulans human alpha-L-fucose + ?
-
 ?
orosomucoid + H2O Niallia circulans low activity, higher activity if desialyzed alpha-L-fucose + ?
-
 ?
saliva + H2O Niallia circulans human alpha-L-fucose + ?
-
 ?
submaxillary mucin + H2O Niallia circulans A-minus type and desialyzed, porcine alpha-L-fucose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
to homogeneity, chromatography techniques Niallia circulans

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Niallia circulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
20
-
-
 Niallia circulans

Storage Stability

Storage Stability Organism
frozen, 1 year Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-fucosyllactose + H2O
-
 Niallia circulans alpha-L-fucose + ?
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose I Niallia circulans alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O 2'-fucosyllactose Niallia circulans alpha-L-fucose + lactose
-
 ?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Niallia circulans alpha-L-fucose + lactose
-
 ?
Fuc-alpha-(1-3)-Gal-beta-(1-4)-Glc + H2O 3-fucosyllactose Niallia circulans alpha-D-fucose + lactose
-
 ?
Gal-beta-(1-4)-GlcNAc-[Fuc-alpha-(1-4)]-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O lacto-N-fucopentaose III Niallia circulans alpha-L-fucose + Gal-beta-(1-4)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
gastric mucin + H2O porcine Niallia circulans alpha-L-fucose + ?
-
 ?
lacto-N-fucopentaose II + H2O
-
 Niallia circulans alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
-
 ?
lactoferrin + H2O low activity Niallia circulans alpha-L-fucose + ?
-
 ?
milk + H2O human Niallia circulans alpha-L-fucose + ?
-
 ?
orosomucoid + H2O low activity, higher activity if desialyzed Niallia circulans alpha-L-fucose + ?
-
 ?
saliva + H2O human Niallia circulans alpha-L-fucose + ?
-
 ?
submaxillary mucin + H2O A-minus type and desialyzed, porcine Niallia circulans alpha-L-fucose + ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 135000, SDS-PAGE Niallia circulans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Niallia circulans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 stable up to Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 in citrate buffer Niallia circulans
6 6.5 in potassium phosphate buffer Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 9 stable within, kept at 4°C Niallia circulans