KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.083 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | - |
Homo sapiens | |
0.125 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | alpha-fucosidase I | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
alpha-fucosidase II, gel filtration | Homo sapiens |
100000 | - |
gel filtration, alpha-fucosidase I | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | Homo sapiens | lacto-N-fucopentaose I | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | Homo sapiens | 2'-fucosyllactose | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-6)-GlcNAc + H2O | Homo sapiens | - |
alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
lacto-N-fucopentaose II + H2O | Homo sapiens | preferentially hydrolysed by alpha-fucosidase II | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | alpha-fucosidase I: sialoglycoprotein, mannose-rich: alpha-fucosidase II | Homo sapiens |
Purification (Comment) | Organism |
---|---|
to homogeneity, two forms: alpha-fucosidase I and II, affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
kidney | - |
Homo sapiens | - |
liver | - |
Homo sapiens | - |
spleen | - |
Homo sapiens | - |
urine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Homo sapiens | 4-methylumbelliferone + alpha-L-fucose | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O | lacto-N-fucopentaose I | Homo sapiens | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? | |
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O | 2'-fucosyllactose | Homo sapiens | alpha-L-fucose + lactose | - |
? | |
Fuc-alpha-(1-6)-GlcNAc + H2O | - |
Homo sapiens | alpha-L-fucose + N-acetyl-D-glucosamine | - |
? | |
lacto-N-fucopentaose II + H2O | preferentially hydrolysed by alpha-fucosidase II | Homo sapiens | alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha-fucosidase I, 2 * 50000, SDS-PAGE, can aggregate to tetra- and hexamers | Homo sapiens |
monomer | alpha-fucosidase II, 1 * 50000, SDS-PAGE, less-biologically active form, can aggregate to tetra- and hexamers | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
alpha-fucosidase I: 50% loss of activity in 40 min, alpha-fucosidase II: 20% loss of activity in 40 min | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
alpha-fucosidase II | Homo sapiens |
5.3 | - |
alpha-fucosidase I | Homo sapiens |