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Literature summary for 3.2.1.51 extracted from
- An alpha-L-fucosidase potentially involved in fertilization is present on Drosophila spermatozoa surface (2006), Mol. Reprod. Dev., 73, 1149-1158.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.125 | - |
4-methylumbelliferyl-alpha-L-fucopyranoside | - |
Drosophila ananassae |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | overlying the acrosome, integral membrane protein terminally mannosylated, with the catalytic site oriented towards the extracellular space | Drosophila ananassae | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 mM, complete inhibition | Drosophila ananassae | |
| Cd2+ | 5 mM, about 5% inhibition | Drosophila ananassae | |
| Co2+ | 5 mM, about 5% inhibition | Drosophila ananassae | |
| Cu2+ | 5 mM, about 5% inhibition | Drosophila ananassae | |
| Fe2+ | 5 mM, 76% inhibition | Drosophila ananassae | |
| Zn2+ | 5 mM, 10% inhibition | Drosophila ananassae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
x * 48000 + x * 55000, SDS-PAGE | Drosophila ananassae |
| 55000 | - |
x * 48000 + x * 55000, SDS-PAGE | Drosophila ananassae |
| 256000 | - |
gel filtration | Drosophila ananassae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Drosophila ananassae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Drosophila ananassae |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| spermatozoon | - |
Drosophila ananassae | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.911 | - |
- |
Drosophila ananassae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 3 weeks, enzyme retains 100% of its activity when stored in presence of 20 mM CHAPS | Drosophila ananassae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl-alpha-L-fucopyranoside + H2O | - |
Drosophila ananassae | methylumbelliferone + alpha-L-fucose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 48000 + x * 55000, SDS-PAGE | Drosophila ananassae |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
30 min, stable | Drosophila ananassae |
| 45 | - |
30 min, 40% loss of activity | Drosophila ananassae |
| 50 | - |
30 min, complete loss of activity | Drosophila ananassae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Drosophila ananassae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 6.3 | pH 3.5: about 55% of maximal activity, pH 6.25: 50% of maximal activity | Drosophila ananassae |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Drosophila ananassae | acidic form | - |
5.7 |
| Drosophila ananassae | neutral form | - |
6.2 |
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